Determination of Antimicrobial, Antioxidant and Antidiabetic Effects of Viscum Album (Mistletoe) Extracts

Year 2020, , 380 - 394, 15.12.2020

Dorukhan Köse Filiz Gökçenaz Yiğit Gülüşan Tuncay Kadriye Özcan

https://doi.org/10.31466/kfbd.808898

Cited By: 1

Abstract

Many plants in nature are rich in phenolic compounds and have natural antioxidant and antimicrobial properties. Therefore, plants have started to gain importance in the biotechnological field and are preferred as alternative sources for the treatment of chronic diseases. Today, it is thought that herbs with antidiabetic properties can be used in the treatment of diabetes, which is an important health problem. Because of this feature, plants have become the focus of researchers in recent years. It is thought that the mistletoe (Viscum album), which is one of these plants and used in the treatment of many diseases, can also be effective for the treatment of diabetes. For this reason, in this study, phenolic and flavonoid substance, antioxidant capacity, antimicrobial activity and enzyme inhibition activity of extracts prepared with three different solvents (chloroform, acetone, ethanol) of mistletoe grown on pear tree collected from Artvin Arhavi region were investigated. When the results obtained from the study are taken into consideration, it can be said that the mistletoe plant, which has a high biological activity today, has a potential of use in the pharmaceutical industry.

Keywords

Viscum album, antimicrobial, antioxidant, enzyme inhibition, diabetes

Viscum Album (Ökse Otu) Ekstrelerinin Antimikrobiyal, Antioksidan ve Antidiyabetik Etkilerinin Belirlenmesi

Abstract

Doğada bulunan birçok bitki, fenolik bileşik yönünden zengin olup doğal antioksidan ve antimikrobiyal özellikler taşımaktadır. Bu nedenle, bitkiler biyoteknolojik alanda önem kazanmaya ve kronik hastalıkların tedavisi için alternatif kaynak olarak tercih edilmeye başlanmıştır. Günümüzde önemli bir sağlık sorunu olan diyabet hastalığının tedavisinde anti-diyabetik özellik taşıyan bitkilerin kullanılabileceği düşünülmektedir. Bu özelliğinden dolayı bitkiler, son yıllarda araştırmacıların odak konusu haline gelmiştir. Bu bitkilerden birisi olan ve birçok hastalığın tedavisinde kullanılan ökse otunun (Viscum album), diyabet hastalığının tedavisi için de etkili olabileceği düşünülmüştür. Bu sebeple, bu çalışmada Artvin Arhavi bölgesinden toplanan armut ağacı üzerinde yetişen ökse otu bitkisinin üç farklı çözgen (kloroform, aseton, etanol) ile hazırlanmış ekstrelerinin fenolik ve flavonoid madde, antioksidan kapasite, antimikrobiyal aktivite ve enzim inhibisyon aktivite tayinleri yapılmıştır. Çalışmadan elde edilen sonuçlar göz önüne alındığında günümüzde yüksek bir biyolojik aktiviteye sahip olan ökse otu bitkisinin ilaç endüstrisinde kullanım potansiyelinin olduğu söylenebilir.

Keywords

Viscum album, antimikrobiyal, antioksidan, enzim inhibisyon, diyabet

References

• Acet, T., ve Özcan, K. (2018). Aslanpençesi (Alchemilla Ellenbergiana) ekstrelerinin antioksidan ve antimikrobiyal özelliklerinin belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 8 (1), 113-121.
• Alberti, K.G., and Zimmet, P.F. (1998). Definition, Diagnosis And Classification Of Diabetes Mellitus And İts Complications. Part 1: Diagnosis And Classification Of Diabetes Mellitus. Provisional Report Of A WHO Consultation. Diabetic Medicine, 15(7), 539-553.
• Barney, C.W., Hawksworth F.G. and Geils B.W. (1998). Hosts of Viscum album. European Journal of Forest Pathology, 28, 187-208.
• Bock, P.R., Friedel, W.E., Hanisch, J., Karasmann, M., and Schneider, B. (2004). Efficacy and safety of long-term complementary treatment with standardized european mistletoe extract (Viscum album L.) in addition to the conventional adjuvant oncologic therapy in patients with primary nonmetastasized mammary carcinoma/results of a multicenter, comparative, epidemiological cohort study in Germany and Switzerland. Arzneimittel Forschung. 54, 456-466.
• Calder, M., and Bernhardt, P. (1983). The biology of mistletoes. Fifth Avenue New York, US Acedemic Press Inc. 3, 23-25.
• Clinical and Laboratory Standards Institute (CLSI), 2017. Performance Standards for Antimicrobial Susceptibility Testing; 27th Informational Supplement. CLSI/ NCCLS, 27th ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA.
• Çubuk, G., and İnce, S. (2015). Oral Antidiabetic Drugs, Kocatepe Veterinary Journal, 8 (1), 95-102.
• Dabbas, M., Kitahara, K., Suganuma, T., Hashimoto, F., and Tadera, K. (2006). Antioxidant and α-amylase inhibitory compounds from Aerial parts of Varthemia iphionoides Boiss, Bioscience Biotechnology and Biochemistry, 70, 2178-2184.
• Duman, E., ve Keser A. (2018). Saponinler ve Diabetes Mellitus Üzerine Potansiyel Etkileri. Ankara Sağlık Bilimleri Dergisi, 7(1), 50-60.
• Ergun, F., Deliorman, D., ve Şener, B. (1994). Viscum album L. (Ökseotu) (Loranthaceae) Bitkisinin Morfolojik Özellikleri ve Türkiye’deki Yayılışı Hakkında Bazı Araştırmalar. Ot Sistematik Botanik Dergisi, 1(2),47-62.
• Ertürk, Ö. (2003). Antimicrobial Activity of Viscum album L. Subsp. Abietis (wiesb). Turkish Journal Of Biology, 27 (4), 255-25.
• Franz, H. (1985). lnhaltsstoffe der Mistel (Viscum album L.) als Potentielle Arzneimittel, Pharmazie. 40(2), 97-103.
• Fukunaga, T., Kajikawa, I., Nishiya, K., Watanabe, Y., Sılzskı, N., Takeya, K., and Itokowa, H. (1983). Studies on the constittıenis of the Europan mistletoe. Viscum Album L. iI, Chem. Pharm. Bull., 36 (3),1185-1189.
• Gölse, J. (1955). Precis de Matière Medicale, Paris Fransa: G. Doin: Cie, Pari
• Hofstetter, M. (1985). HPLC-Charakterisierung von Lektinen der Mistel (Viscum album L.) und Verbreitung der Pflanze in der Schweiz, Doktora Tezi, Eidgenössischen Technischen Hochschule Zürich, Zürich
• Khıvaja, T.A., Dıas, C.B., and Pentecost, S. (1986). Recent studies on the anticacer activities of mistletoe (Viscum Album L) and its alkaloids. Oncology, 13(1), 42-50.
• Kirby, A.J., and Schmidt, R.J. (1997). The antioxidant activity of Chinese herbs for eczema and of placebo herbs. Journal of Ethnopharmacol. 56 (2), 103-108.
• Kumar, K., Saraswathy, A., Amerjoth, S., and Ravishankar, B. (2014). Journal of tranditional and complemantary medicine, 4(4), 258-262
• Laube, H. (2002). Acarbose An Update Of İts Therapeutic Use İn Diabetes Treatment. Clinical Drug Investigation, 22 (3), 141–156.
• Miller, A.G. (1982). Viscum album L. Flora of Turkey and the East Aegean Islands. Edinburgh University Press, 547-548
• Moreno, M.I.N., Isla, M.I., Sampietro, A.R., and Vattuone, M.A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal Ethnopharmacology, 71, 1-2, 109-114.
• NagI, W., and Stein, B. (1989). DNA Characterization in Host- Spesific Viscum album Subspecies (Viscaceae), Plant Syst. Evol, 166 (3-4), 243-248.
• Nam, J.S., Sharma, AR., Nguyen, L.T., Chakraborty, C., Sharma, G., and Lee, S.S. (2016). Application of bioactive quercetin in oncotherapy from nutrition to nanomedicine. MDPI Molecules 21 (1) 108.
• Oboh, G., Babatola, LJ., and Ademiluyi, AO. (2018). In vitro inhibitory effects of mistletoes (Loranthus begwensis L.) phenolic-rich extracts on a-amylase, a-glucosidase, and angiotensin converting enzyme activities. Journal Food Biochem,42 (4) 12504-12508
• Ohikhena, F.U., Wintola, O.A., and Afolayan, A.J. (2017). Evaluation of the Antibacterial and Antifungal Properties of Phragmanthera capitata (Sprengel) Balle (Loranthaceae), a Mistletoe Growing on Rubber Tree, Using the Dilution Techniques. The Scientific World Journal, 1-8
• Orhan, N., Hoçbaç, S., Orhan, D., Asya, M., ve Ergun, F. (2014). Haziran Türkiye'nin bazı antidiyabetik bitkilerinin enzim inhibitör ve radikal süpürücü etkileri Journal Basic Med. Sci. 17 (6), 426-432.
• Pietrzak, W., Nowak, R., Gawlik-Dziki, U., Lemieszek, M., and Rzeski, W. (2017). LC-ESI-MS/MS Identification of Biologically Active Phenolic Compounds in Mistletoe Berry Extracts from Different Host Trees. MDPI Molecules 22 (4), 624 doi:10.3390 / molecules22040624
• Rahabasa-Lhoret, R., and Chıasson, J. L. (2009). α-Glucosidase inhibitors. International Textbook of Diabetes Mellitus, 1,(3) 901-914.
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., and Rice-Evans, C. (1999). Antioxidant activity applying and improved ABTS radical cation decolarization assay. Free Radic Biol Med, 26 (9-10), 1231-1237.
• Roman, G.P., Neagu, E., Radu, G.L. (2009). Antiradical Activities of salvia Officinalis and Viscum album L. Extracts Concentrated by ultrafiltration process. Acta Sci. Pol., Technol. Aliment. 8(3), 47-58.
• Shinde, A., Ganu, J., and Naik, P. (2012). Effect of free radicals & Antioxidants on oxidative stress. J Dent Allied Sci. ,1(2), 63- 66.
• Slinkard, K., and Singleton, V.L. (1977). Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28, (1) 49- 55.
• Stefanucci, A., Zengin, G., and Martinez, E.J. (2020). Viscum album L. homogenizer-assisted and ultrasound-assisted extracts as potential sources of bioactive compounds. Journal of Food Biochem. 44 (9), https://doi.org/10.1111/jfbc.13377
• Uçar, Ö., Karagöz, A., ve Arda, N. (2006). Viscum album ssp. antioksidan aktivitesi. Fitoterapia, 77 (7-8), 556-560.
• Wagner, H., Feil, B., Seligmann, O., Petricic, I., and Kalogjera, Z. (1986). Phenylpropanes and lignans of Viscum Album L cardioactive drugs V., Planta Med., (2), 702-704.
• WHO, (1980). Expert Committee On Diabetes Mellitus. Technical Report Series, World Health Organization, Geneva.
• Yang, X.W., Huang, M.Z., Jin, Y.S., Sun, L.N., Song, Y., and Chen, H.S. (2012). Phenolics from Bidens bipinnata and their amylase inhibitory properties. Fitoterapia 83 (7) , 1169– 1175.
• Yıldız, S., Gürgen, A., Kılıç, C., Tabbouche, S., Kılıç, A., ve Can, Z. (2019). Antioxidant, Antimicrobial, and Anti-Quorum Sensing Activities of Usnea filipendula and Viscum album. Anadolu Çev. ve Hay. Dergisi, 4(4), (613-620).