Comparison of KRAS Mutation Status with Clinical Parameters in Colon Adenocarcinoma

Year 2021, , 648 - 662, 15.12.2021

Barış Boylu , Mustafa Türkmen

https://doi.org/10.31466/kfbd.981849

Abstract

KRAS mutations are mutually exclusive with other activating mutations on EGFR pathway. Detection of KRAS mutations associated with tumorigenesis, predicates the lack of other mutations on the same pathway and shows that the application of targeted therapy approaches which target other proteins in EGFR-MAPK pathway ineffective. In this study, frequency of KRAS mutations in colorectal cancer and relationship between KRAS mutation status and other clinical features were assessed. KRAS mutations were detected in 47,7% of the cases included in our study. We determined that 76% of the mutations were located in codon 12, 9% of the mutations were located in codon 13, 9% of the mutations were located in codon 61 and 6% of the mutations located in codon 117 or codon 146. Determination of mutation rates and association of mutations with clinical features for different populations are important for planning of the treatment strategies nationwide. In our study, we have demonstrated that KRAS mutation status and clinical features associated with KRAS mutation is in accordance with the literature. We have determined that there is statistically significant correlation between grade and KRAS mutation status.

Keywords

Colorectal cancer, KRAS oncogene, Anti-EGFR treatment

Kolon Adenokarsinomlarında KRAS Mutasyon Durumunun Klinik Veriler ile Karşılaştırılması

Abstract

KRAS mutasyonu EGFR yolağındaki diğer aktifleştirici mutasyonlarla birbirini dışlayan özellik göstermektedir. Tümörijenez ile bağlantılı KRAS mutasyonlarının tespiti, aynı yolak üzerinde başka mutasyonların yokluğunu da büyük ölçüde göstermekte ve dolayısıyla EGFR-MAPK yolağı üzerindeki başka proteinleri hedefleyen akıllı ilaçların kullanımının fayda sağlamayacağını göstermektedir. Bu çalışmada kolorektal kanser vakalarında KRAS mutasyonu görülme sıklığı ve mutasyon durumunun diğer klinik veriler ile ilişkisi incelenmiştir. KRAS mutasyonu çalışmamıza dahil edilen vakaların %47,7’sinde tespit edilmiştir. KRAS mutasyonlarının %76’sının 12. kodonda, %9’unun 13. kodonda, %9’unun 61. kodonda ve %6’sının 117. veya 146. kodonda gerçekleştiği tespit edilmiştir. Farklı populasyonlarda değişebilen mutasyon oranlarının ve mutasyonların klinik özelliklerle ilişkisinin tespit edilmesi ulusal tedavi stratejilerinin planlanması açısından büyük öneme sahiptir. Çalışmamızda KRAS mutasyon durumunun ve KRAS mutasyonu ile bağlantılı klinik özelliklerin literatür ile uyumlu olduğu gösterilmiştir. Çalışmamızda KRAS mutasyon durumu ile tümör grade’i arasında istatistiki olarak anlamlı korelasyonun varlığı tespit edilmiştir.

Keywords

Kolorektal kanser, KRAS onkogeni, Anti-EGFR tedavisi

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