DPPH (1,1-diphenyl-2-picrylhydrazyl) Free Radical Sweeping Activity of Scenedesmus sp.

Year 2017, Volume: 7 Issue: 2, 146 - 151, 15.12.2017

Elif Neyran Soylu , Ayşegül Çebi

https://doi.org/10.31466/kfbd.353777

Cited By: 1

Abstract

Spectrophotometric DPPH method was used for the determination
of DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of Scenedesmus sp. which was isolated from
the Giresun mine and cultivated under BG11 culture conditions. 1 mL of 10-3 M
DPPH ethanol solution was added and 3 mL of extract solution was added and
vortexed. The absorbance was read at 517 nm for 30 minutes in the dark. BHT and
Trolox were used as standard antioxidants. The DPPH radical scavenging activity
reaction was calculated using the formula.

Keywords

Scenedesmus sp, DPPH, algae culture extraction

Scenedesmus sp.’nin DPPH (1,1-difenil-2-pikrilhidrazil) Serbest Radikal Süpürücü Aktivitesi

Abstract

Giresun ili derelerinden izole edilen ve kültür
şartları altında BG11 besi yerinde yetiştirilen Scenedesmus sp. türünün DPPH (1,1-difenil-2-pikrilhidrazil) serbest
radikal süpürücü aktivite tayini için spektrofotometrik DPPH yöntemi
kullanılmıştır. 10-3 M DPPH etanol çözeltisinden 1 mL alınıp 3 mL ekstrakt
çözeltisi ilave edilip vorteks ile çalkalanmıştır. 30 dakika karanlıkta
bekletilip 517 nm’de absorbans okunmuştur. Standart antioksidan olarak BHT ve
Troloks kullanılmıştır. DPPH radikalini süpürme aktivitesi formül kullanılarak
hesaplanmıştır.

Keywords

DPPH, Scenedesmus sp., alg kültür ekstraksiyonu

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