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DPPH (1,1-diphenyl-2-picrylhydrazyl) Free Radical Sweeping Activity of Scenedesmus sp.

Year 2017, Volume: 7 Issue: 2, 146 - 151, 15.12.2017
https://doi.org/10.31466/kfbd.353777

Abstract

Spectrophotometric DPPH method was used for the determination
of DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of Scenedesmus sp. which was isolated from
the Giresun mine and cultivated under BG11 culture conditions. 1 mL of 10-3 M
DPPH ethanol solution was added and 3 mL of extract solution was added and
vortexed. The absorbance was read at 517 nm for 30 minutes in the dark. BHT and
Trolox were used as standard antioxidants. The DPPH radical scavenging activity
reaction was calculated using the formula.

References

  • Akyıl S., İlter I., Koç M. ve Kaymak-Ertekin F. 2016. Alglerden elde edilen yüksek değerlikli biyoaktif/Biyolojik Uygulama Alanları Akademik Gıda, 14 (4) : 418-423.
  • Brand-Williams, W., Cuvelier M. E. ve Berset C. 1994. Use of free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft & Technologie, 28: 25-30.
  • Britton G. 1995. Structure and properties of carotenoids in relation of function. FASEB J., 9:1551–1558.
  • El-Sheekh M. M., Osman M. E. H., Dyab M. A. ve Amer M. S. 2006. Production and characterization of antimicrobial active substance from the Cyanobacterium Nostoc muscorum. Environmental Toxicology and Pharmacology, 21(1): 42–50.
  • Fagali N. ve Catalá A. 2008. Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH radical dot techniques. Biophys. Chem., 137: 56–62.
  • Guedes A., Amaro H. M. ve Malcata F. X. 2011. Microalgae as sources of high added‐value compounds a brief review of recent work. Biotechnology Progress, 27(3): 597-613.
  • Halim R., Danquah M. K., Webley P. A. 2012. Extraction of oil from microalgae for biodiesel production: A review. Biotechnol. Adv., 30: 709–732.
  • Herrero M., Cifuentes A. Ve Ibañez E. 2006. Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food by-products, algae and microalgae—A review. Food Chem., 98:136–148.
  • Tandeau-de-Marsac N. ve Houmard J. 1993. Adaptation of cyanobacteria to environmental stimuli: New steps towards molecular mechanisms. FEMS Microbiol. Rev., 104: 119–190.
  • León R., Martín M., Vigara J., Vilchez C. ve Vega J. 2003. Microalgae-mediated photoproduction of β-carotene in aqueous organic two-phase systems. Biomol. Eng., 20: 177–182.
  • Mendiola J. A., Jaime L., Santoyo S., Reglero G., Cifuentes A., Ibañez E. ve Señoráns F.J. 2007. Screening of functional compounds in supercritical fluid extracts from Spirulina platensis. Food Chem., 102:1357–1367.
  • Mueller L. ve Boehm, V. 2011. Antioxidant Activity of β-Carotene Compounds in Different in vitro Assays. Molecules, 16: 1055–1069.
  • Ngo D. H., Wijesekara I., Vo T. S., Van Q., Ta S. ve Kim K. 2010. Marine food-derived functional ingredients as potential antioxidants in the food industry: An overview. Food Research International, 44: 523-529.
  • Shalaby E. A., Shanab S. M. M. 2013.Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J. Geo-Mar. Sci., 42: 556–564.
  • Srivastava A., Bhargava P. ve Rai L. 2005. Salinity and copper-induced oxidative damage and changes in the antioxidative defense systems of Anabaena doliolum. World J. Microbiol. Biotechnol., 21: 1291–1298.
  • Pulz O., Gerbsch N. ve Bacholz R. 1995.Light energy supply in plate-type and light diffusing optical fiber bioreactors. J. Appl. Phycol., 7:145–149.
  • Wiltshire K. H., Boersma M., Möller A ve Buhtz, H. 2000. Extraction of pigments and fatty acids from the green alga Scenedesmus obliquus (Chlorophyceae). Aquatic Ecology, 34: 119–126.

Scenedesmus sp.’nin DPPH (1,1-difenil-2-pikrilhidrazil) Serbest Radikal Süpürücü Aktivitesi

Year 2017, Volume: 7 Issue: 2, 146 - 151, 15.12.2017
https://doi.org/10.31466/kfbd.353777

Abstract

Giresun ili derelerinden izole edilen ve kültür
şartları altında BG11 besi yerinde yetiştirilen Scenedesmus sp. türünün DPPH (1,1-difenil-2-pikrilhidrazil) serbest
radikal süpürücü aktivite tayini için spektrofotometrik DPPH yöntemi
kullanılmıştır. 10-3 M DPPH etanol çözeltisinden 1 mL alınıp 3 mL ekstrakt
çözeltisi ilave edilip vorteks ile çalkalanmıştır. 30 dakika karanlıkta
bekletilip 517 nm’de absorbans okunmuştur. Standart antioksidan olarak BHT ve
Troloks kullanılmıştır. DPPH radikalini süpürme aktivitesi formül kullanılarak
hesaplanmıştır.

References

  • Akyıl S., İlter I., Koç M. ve Kaymak-Ertekin F. 2016. Alglerden elde edilen yüksek değerlikli biyoaktif/Biyolojik Uygulama Alanları Akademik Gıda, 14 (4) : 418-423.
  • Brand-Williams, W., Cuvelier M. E. ve Berset C. 1994. Use of free radical method to evaluate antioxidant activity. Lebensmittel-Wissenschaft & Technologie, 28: 25-30.
  • Britton G. 1995. Structure and properties of carotenoids in relation of function. FASEB J., 9:1551–1558.
  • El-Sheekh M. M., Osman M. E. H., Dyab M. A. ve Amer M. S. 2006. Production and characterization of antimicrobial active substance from the Cyanobacterium Nostoc muscorum. Environmental Toxicology and Pharmacology, 21(1): 42–50.
  • Fagali N. ve Catalá A. 2008. Antioxidant activity of conjugated linoleic acid isomers, linoleic acid and its methyl ester determined by photoemission and DPPH radical dot techniques. Biophys. Chem., 137: 56–62.
  • Guedes A., Amaro H. M. ve Malcata F. X. 2011. Microalgae as sources of high added‐value compounds a brief review of recent work. Biotechnology Progress, 27(3): 597-613.
  • Halim R., Danquah M. K., Webley P. A. 2012. Extraction of oil from microalgae for biodiesel production: A review. Biotechnol. Adv., 30: 709–732.
  • Herrero M., Cifuentes A. Ve Ibañez E. 2006. Sub- and supercritical fluid extraction of functional ingredients from different natural sources: Plants, food by-products, algae and microalgae—A review. Food Chem., 98:136–148.
  • Tandeau-de-Marsac N. ve Houmard J. 1993. Adaptation of cyanobacteria to environmental stimuli: New steps towards molecular mechanisms. FEMS Microbiol. Rev., 104: 119–190.
  • León R., Martín M., Vigara J., Vilchez C. ve Vega J. 2003. Microalgae-mediated photoproduction of β-carotene in aqueous organic two-phase systems. Biomol. Eng., 20: 177–182.
  • Mendiola J. A., Jaime L., Santoyo S., Reglero G., Cifuentes A., Ibañez E. ve Señoráns F.J. 2007. Screening of functional compounds in supercritical fluid extracts from Spirulina platensis. Food Chem., 102:1357–1367.
  • Mueller L. ve Boehm, V. 2011. Antioxidant Activity of β-Carotene Compounds in Different in vitro Assays. Molecules, 16: 1055–1069.
  • Ngo D. H., Wijesekara I., Vo T. S., Van Q., Ta S. ve Kim K. 2010. Marine food-derived functional ingredients as potential antioxidants in the food industry: An overview. Food Research International, 44: 523-529.
  • Shalaby E. A., Shanab S. M. M. 2013.Comparison of DPPH and ABTS assays for determining antioxidant potential of water and methanol extracts of Spirulina platensis. Indian J. Geo-Mar. Sci., 42: 556–564.
  • Srivastava A., Bhargava P. ve Rai L. 2005. Salinity and copper-induced oxidative damage and changes in the antioxidative defense systems of Anabaena doliolum. World J. Microbiol. Biotechnol., 21: 1291–1298.
  • Pulz O., Gerbsch N. ve Bacholz R. 1995.Light energy supply in plate-type and light diffusing optical fiber bioreactors. J. Appl. Phycol., 7:145–149.
  • Wiltshire K. H., Boersma M., Möller A ve Buhtz, H. 2000. Extraction of pigments and fatty acids from the green alga Scenedesmus obliquus (Chlorophyceae). Aquatic Ecology, 34: 119–126.
There are 17 citations in total.

Details

Subjects Engineering
Journal Section Articles
Authors

Elif Neyran Soylu

Ayşegül Çebi

Publication Date December 15, 2017
Published in Issue Year 2017 Volume: 7 Issue: 2

Cite

APA Soylu, E. N., & Çebi, A. (2017). Scenedesmus sp.’nin DPPH (1,1-difenil-2-pikrilhidrazil) Serbest Radikal Süpürücü Aktivitesi. Karadeniz Fen Bilimleri Dergisi, 7(2), 146-151. https://doi.org/10.31466/kfbd.353777