Hatay’da Farklı Bölgelerde Yetiştirilen Zeytin Yapraklarının Antioksidan Özelliklerinin Belirlenmesi

Year 2018, Volume: 8 Issue: 1, 77 - 86, 18.06.2018

Hatice Danahaliloğlu , Yener Tekeli Sezer Göycıncık , Figen Yıldırım

https://doi.org/10.31466/kfbd.405060

Cited By: 3

Abstract

Bu çalışmada, Hatay’da farklı bölgelerde yetiştirilen
Halhalı türü zeytin (Olea europaea)ağaçlarının
yapraklarının metanol ekstraktlarının antioksidan aktiviteleri belirlenmiştir.
Toplam fenolik madde miktarları Folin-Ciocaltaeu yöntemi ile tayin edilmiştir.
Antioksidan özelliklerini belirlemek için kullanılan diğer yöntemler DPPH  radikal süpürme yöntemi, FRAP  indirgeme gücü metodu  ve β-karoten-linoleik asit emülsiyon
yöntemidir. Sonuçlar standart antioksidanlar ile (BHA, BHT) kıyaslanmıştır.
İncelenen tüm bölgelerin zeytin yapraklarının antioksidan aktiviteye sahip
olduğu belirlenmiştir.

Keywords

Zeytin yaprağı, toplam fenolik madde, antioksidan aktivite, dpph

Determination of Antioxidant Properties of Olive Leaves Grown in Different Regions in Hatay

Abstract

In this study; antioxidant activities of metanol extracts of olive leaf (Olea eueopaea) which is grown in different parts
of Hatay were determined. Total phenolic contents were determined by the Folin-Ciocaltaeu method. The other
methods used to determine the antioxidant characteristics were 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical
scavenging method, ferric ion reducing power (FRAP) and β-carotene-linoleic acid emulsion method. The results were
compared with the standard antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). It was
determined that olive leaves in all regions examined had antioxidant activity.

Keywords

Olive leaf, total phenolic content, antioxidant activity, DPPH

References

• Referans 1 Agalias A., Melliou E., Magiatis P., Mitaku S., Gikas E., Tsarbopoulos A. (2005). Quantitation of oleuropein and related metabolites in decoctions of Olea europaea leaves from ten Greek cultivated varieties by HPLC with diode array detection (HPLC-DAD), Journal of Liquid Chromatography & Related Technologies, 28,1557-1571.
• Referans 2 Aytul, K.K., (2010). Zeytin Yaprağı Özütünün Antimikrobiyal ve Antioksidan Aktivitileri ve Gıda Uygulamaları. Doktora tezi, İzmir Yüksek Teknoloji Enstitüsü İzmir.
• Referans 3 Bahloul, N., Nourhene, B., Kouhıla, M., Kechaou, N., (2009). Effect of Convective Solar Drying On Colour, Total Phenols and Radical Scavenging Activity of Olive Leaves (Olea Europaea L.). International Journal of Food Science and Techonology, 44, 2561-2567
• Referans 4Bouazız, M., Fekı, I., Ayadı, M., Jemaı, H., Sayadı, S., (2010). Stability of Refined Olive Oil and Olive-Pomace Oil Added by Phenolic Compunds From Olive Leaves. European Journal of Lipid Science and Technology, 112,894-905.
• Referans 5 Boudhrioua, N., Bahloul, N., Slımen, B.I., Kechaou, N., (2009). Comparison on The Total Phenol Contents and The Color of Fresh and Infrared Dried Olive Leaves. Industrial Crops and Products, 29, 412-419
• Referans 6 Brand-Williams, W., Cuvelier, M. E., Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food Science and Technology, 28, 25-30.
• Referans 7 Capasso, F., Simone F., Senatore F. 1982. Traditional phytotherapy in the Agri Villey, Lucania, Southern Italy. Journal of Ethnopharmacology, 6,243-250.
• Referans 8 Castro, M.D.L., Capote, F.P., (2010). Extraction of Oleuropein and Related Phenols From Olive Leaves and Branches. Olives and Olive Oil in Health Disease Prevention, 28,259-273
• Referans 9 Doğançay, G. (2013). Sulanan ve Sulanmayan Koşullarda Bazı Zeytin Çeşitlerinin Yapraklarındaki Biyoaktif Bileşiklerin Mevsimsel Değişimi. Yüksek Lisans Tezi, Yedi Aralık Üniversitesi, Fen bilimleri Enstitüsü, Kilis.
• Referans 10 El-Nehir, S., Karakaya, S., (2009). Olive Tree (Olea europaea) Leaves: Potential Beneficial Effects on Human, Nutrition Reviews, 11,632-638
• Referans 11 Erbay, Z., İçier, F., (2008). Zeytin Ağacından Faydalanmanın Yeni Bir Yolu Olarak Zeytin Yaprağı ve Gıda Endüstrisindeki Potansiyel Uygulama Alanları, Akademik Gıda 6 (3), 27-36
• Referans 12 Ferreıra, I.C.F.R., Barros, L., Soares, M.E., Bastos, M.L., Pereıra, J.A., (2007). Antioxidant Activity and Phenolic Contents of Olea europaea L. Leaves Sprayed with Different Copper Formulations, Food Chemistry, 103,188-195
• Referans 13 Guinda, A., Albi, T., Perez – Camino, M., Lanzon, A., (2004). Supplementation of oil with oleanolic acid from olive leaf (Olea europea), European Journal of Lipid Science and Technology, 106, 22-26.
• Referans 14 Harp, F., (2011). Gemlik, Domat, Adana Topağı ve Adana Yerli Zeytin Yapraklarının Antioksidan Etkilerinin Belirlenmesi, Yüksek Lisans Tezi, Çukurova Üniversitesi, Fen bilimleri Enstitüsü, Adana.
• Referans 15 Hue, S.M., Boyce, A.N., Somasundram, C., (2012). Antioxidant activity, phenolic and flavonoid contents in the leaves of different varieties of sweet potato (Ipomoea batatas). Australian Journals of Crop Science 6(3),375-380.
• Referans 16 Jemai, H., El Fekı, A., Sayadı, S., (2009). Antidiabetic and Antioxidant Effect of Hydroxytyrosol and Oleuropein from Olive Leaves in Alloxan-Diabetic Rats, Journal Agricultural and Food Chemistry, 57, 8798-8804
• Referans 17 Kaur, C. and Kapoor, H.C. (2002). Anti-oxidant activity and total phenolic content of some Asian vegetables. International Journal of Food Science & Technology, 37:153-161.
• Referans 18 Oyaizu, M., (1986). Studies on product of browning reaction prepared from glucose amine, Japanese Journal of Nutrition, 44, 307–315
• Referans 19 Salah, M. B. Abdelmelek H., Abderraba M. (2012). Study of Phenolic Composition and Biological Activities Assessment of Olive Leaves From Different Varieties Grown in Tunisia, Medicinal Chemistry, 2, 5
• Referans 20 Silva, S., Gomes, L., Leitao, F., Coelho, A.V., Vilas Boas, L., (2006). Phenolic Compounds and Antioxidant Activity of Olea europaea L. Fruits and Leaves, International Food Science and Techonology, 12, 385-396
• Referans 21 Tsimidou, M.Z., Papoti, P.V., (2010). Bioactive Ingredients in Olive Leaves, Olives and Olive Oil in Health and Disease Prevention, Chapter 39, 349-356
• Referans 22 Ziyyat A., Legssyer A., Mekhfi H., Dassouli A., Serhrouchni M., Benjelloun W. (1997). Phytotherapy of hypertension and diabetes in oriental Moracco, Journal of Ethnopharmacology, 58, 45-54.