Lightening the Antioxidant and Antimicrobial Properties of the Heracleum pastinacifolium and its Phenolic Content

Year 2022, Volume: 12 Issue: 1, 22 - 32, 15.06.2022

Tuba Acet Kadriye Özcan

https://doi.org/10.31466/kfbd.910956

Cited By: 1

Abstract

Heracleum species, known as hogweed, are traditionally used as food additives, spices and flavorings. In addition, it is known to have ethnobotanical properties. In this study, the total phenolic and flavonoid content and antioxidant capacity and antimicrobial activities of the extracts of endemic Heracleum pastinacifolium obtained with different solvents (ethanol, methanol and ethyl acetate) were investigated by spectrophotometric methods. In addition, the phenolic component analysis was analyzed by HPLC. According to the findings, the antioxidant and antimicrobial properties of the methanol extract compared to other extracts were found to be remarkable. The main phenolic components of the methanol extract were determined to be kaempherol (726.05 µg / g extract) and quercetin (451.6 µg / g extract). As a result of the data obtained, it can be said that the plant has an industrial use potential. However, it is important to support the data with further studies and to determine the effects of active ingredients on living things by purifying them. 

Keywords

Antimicrobial, Antioxidant, Phenolic content, Heracleum pastinacifolium

Heracleum pastinacifolium’nın Antioksidan ve Antimikrobiyal Özellikleri İle Fenolik İçeriğinin Aydınlatılması

Abstract

Yaban otu olarak bilinen Heracleum türleri, geleneksel olarak gıda katkı maddesi, baharat ve tatlandırıcı olarak kullanılmaktadır. Bunun yanı sıra, etnobotanik özellikleri olduğu da bilinmektedir. Bu çalışmada, endemik Heracleum pastinacifolium’un farklı çözücülerle (etanol, metanol ve etil asetat) elde edilen özütlerinin toplam fenolik ve flavonoid içeriği ile antioksidan ve antimikrobiyal aktiviteleri spektrofotometrik yöntemlerle araştırılmıştır. Ayrıca, fenolik bileşen analizi HPLC ile analiz edilmiştir. Bulgulara göre, metanol özütünün diğer özütlere kıyasla antioksidan ve antimikrobiyal özelliklerinin dikkat çekici olduğu bulunmuştur. Metanol özütünün başlıca fenolik bilşenlerinin kaemferol (726.05 µg/g özüt) ve kuersetin (451.6 µg/g özüt) olduğu tespit edilmiştir. Elde edilen veriler ışığında, bitkinin endüstriyel kullanım potansiyeli olduğu söylenebilir. Bununla birlikte, daha ileri çalışmalarla verilerin desteklenmesi ve aktif bileşenlerin saflaştırılarak canlılar üzerindeki etkilerinin belirlenmesi önem arz etmektedir. 

Keywords

Antimikrobiyal, Antioksidan, Fenolik içerik, Heracleum pastinacifolium

References

• Acet, T. (2021) Chemical profile, antioxidant, antimicrobial, and enzyme inhibition activities of Pilosella hoppeana subsp. cilicica, Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, DOI: 10.1080/11263504.2021.1894257.
• Acet, T. (2021). Determining the phenolic components by using HPLC and biological activity of Centaurea triumfetti, Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, 155(1), 159-164.
• Acet, T., Ozcan, K. and Zengin, G. (2020). An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum. Journal of Food Biochemistry, 44, e13128.
• Akbaribazm, M., Goodarzi, N., Rahimi, M., Naseri, L. and Khazaei, M. (2021). Anti-inflammatory, anti-oxidative and anti-apoptotic effects of Heracleum persicum L. extract on rats with gentamicin-induced nephrotoxicity. Asian Pacific Journal of Tropical Biomedicine, 11, 47-58.
• Akmeşe, O., Acet, T. ve Özcan, K. (2020). Elazığ İlinde Yetişen Morus nigra L.’nin Antioksidan ve Antimikrobiyal Aktivitelerinin ve Antibiyotiklerle Sinerjistik Etkisinin Belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10 (4), 983-995.
• Bahadori, M.B., Dinparast, L. and Zengin, G. (2016). The Genus Heracleum: A Comprehensive Review on Its Phytochemistry, Pharmacology, and Ethnobotanical Values as a Useful Herb. Comprehensive Reviews In Food Science And Food Safety, 15,1018-1039.
• Buruk, K., Sokmen, A., Aydin, F. and Erturk, M. (2006). Antimicrobial activity of some endemic plants growing in the eastern Black Sea region, Turkey. Fitoterapia, 77, 388–91.
• Clinical and Laboratory Standards Institute (CLSI), 2017. Performance Standards for Antimicrobial Susceptibility Testing; 27th Informational Supplement. CLSI/NCCLS, 27th ed.; Clinical and Laboratory Standards Institute: Wayne, PA, USA.
• Çoruh, N., Sağdıçoğlu Celep, A.G. and Özgökçe, F. (2007). Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food Chemistry, 100(3), 1237-1242.
• Erastus Nembu Nembo, E. N., Hescheler, J. and Nguemo, F. (2020). Stem cells in natural product and medicinal plant drug discovery—An overview of new screening approaches, Biomedicine and Pharmacotherapy, 131, 110730.
• Fakhri, S., Abbaszadeh, F., Jorjani, M. and Pourgholami, M. H. (2021). The effects of anticancer medicinal herbs on vascular endothelial growth factor based on pharmacological aspects: a review study. Nutrition and Cancer, 73(1), 1-15.
• Faustino, M. V., Faustino, M. A. F. and Pinto, D. C. G. A. (2019). Halophytic grasses, a new source of nutraceuticals? A review on their secondary metabolites and biological activities. International Journal of Molecular Sciences, 20, 1067.
• Firuzi, O., Asadollahi, M., Gholami, M. and Javidnia, K. (2010). Composition and biological activities of essential oils from four Heracleum species. Food Chemistry, 122, 117–22.
• Iscan, G., Ozek, T., Ozek, G.,Duran, A. and Baser, K. H. C. (2004). Essential oils of three species of Heracleum. Anticandidal activity. Chemistry of Natural Compounds, 40, 544-547.
• Kalkan, S., Engin, M. S. ve Otağ, M. R. (2021). Kırmızı hevhulma (Lythrum salicaria l.) bitkisinin toplam fenolik bileşik tayini ile antioksidan ve antimikrobiyel aktivitelerinin belirlenmesi. Gümüşhane Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 11(1), 219-228.
• Kalkan, S., Otağ, M. R. and Engin, M.S. (2020). Physicochemical and bioactive properties of edible methylcellulose films containing Rheum ribes L. extract. Food Chemistry, 307, 125524.
• Köseoğlu, D., Yiğit, F. G., Tuncay, G. ve Özcan, K. (2020). Viscum album (Ökse Otu) Ekstrelerinin Antimikrobiyal, Antioksidan ve Antidiyabetik Etkilerinin Belirlenmesi. Karadeniz Fen Bilimleri Dergisi, 10(2), 380-394.
• Kotiloğlu, D., Acet, T. and Özcan, K. (2020). Phytochemical profile and biological activity of a therapeutic orchid from Anatolia: Dactylorhiza romana subsp. georgica. Journal of Food Measurement and Characterization, 14, 3310–3318.
• Orhan, I.O., Tosun, F. and Skalıcka-Woźnıak, K. (2016). Cholinesterase and tyrosinase inhibitory, and antioxidant potential of randomly selected Umbelliferous plant species and the chromatographic profile of Heracleum platytaenium Boiss. and Angelica sylvestris L. var. sylvestris. Journal of the Serbian Chemical Society, 81(4), 357-368.
• Özcan, K. (2020). Antibacterial, antioxidant and enzyme inhibition activity capacities of Doronicum macrolepis (FREYN&SINT): An endemic plant from Turkey. Saudi Pharmaceutical Journal, 28(1), 95-100.
• Özcan, K. and Acet, T. (2018). In vitro antioxidant and antimicrobial activities of the five different solvent extracts of Centaurea pulcherrima var. freynii from Turkey. Fresenius Environmental Bulletin, 27 (6), 4047-4051.
• Rates, S. M. K. (2001). Plants as source of drugs. Toxicon 39, 603–613.
• Romano, B., Lucariello, G. and Capasso, R. (2021). Topical Collection “Pharmacology of Medicinal Plants”. Biomolecules 11 (1), 101.
• Soliman, S., Hamoda, A. M., El-Shorbagi, A. N. A. and El-Keblawy, A.A. (2019). Novel betulin derivative is responsible for the anticancer folk use of Ziziphus spina-christi from the hot environmental habitat of UAE. Journal Ethnopharmacology, 231, 403–408.
• Son, H.J., Eo, H.J., Park, G.H. and Jeong, J.B. (2021). Heracleum moellendorffii root extracts exert immunostimulatory activity through TLR2/4‐dependent MAPK activation in mouse macrophages, RAW264.7 cells. Food Science and Nutrition, 9, 514– 521.
• Tkachenko, K. (2007). Antiviral activity of the essential oils of some Heracleum L. species. Journal of Herbs, Spices and Medicinal Plants, 12, 1–12.
• Walasek, M., Grzegorczyk, A., Malm, A. and Skalicka-Woźniak, K. (2015). Bioactivity-guided isolation of antimicrobial coumarins from Heracleum mantegazzianum Sommier & Levier (Apiaceae) fruits by high-performance counter-current chromatography. Food Chemistry, 186, 133-138.
• WHO (2002) Traditional Medicine Strategy 2002-2005. Geneva.
• Yuan, H., Ma, Q., Ye, L. and Piao, G. (2016). The Traditional Medicine and Modern Medicine from Natural Products. Molecules, 21(5), 559.
• Zeng, Y., Nikitkova, A., Abdelsalam, H., Li, J. and Xiao, J. (2019). Activity of quercetin and kaemferol against Streptococcus mutans biofilm. Archives of Oral Biology, 98, 9-16.
• Zhang, Q. Y., Wang, F. X., Jia, K. K., and Kong, L. D. (2018). Natural Product Interventions for Chemotherapy and Radiotherapy-Induced Side Effects. Frontiers in pharmacology, 9, 1253.