Antioxidant and antimicrobial activities of the essential oil of Helichrysum chionophilum: an endemic plant of Türkiye

Year 2023, Volume: 13 Issue: 2, 680 - 689, 15.06.2023

Tuba Acet , Kadriye Özcan

https://doi.org/10.31466/kfbd.1271087

Cited By: 1

Abstract

Diseases that develop due to microorganisms have become increasingly dangerous today. Especially in the fight against resistant microorganisms, there is a need for cheaper, natural and effective methods. Plant materials containing biologically active compounds come to the fore in this sense. Therefore, in the present study, the antioxidant and antimicrobial activities of the essential oil obtained from H. chionophilum collected from Gümüşhane were investigated. The essential oil was obtained by hydro-distillation using a Clevenger-type apparatus. Free radical scavenging activity was determined using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. Antimicrobial activity was examined using the microdilution method using 17 bacteria and 2 yeasts. According to the findings, IC50 value of the radical scavenging activity of the essential oil of H. chionophilum plant was found to be 260.0±0.2 µg/mL. Also, the essential oil of the plant showed outstanding antimicrobial activity against Y. pseudotuberculosis (MIC: 16 µg/mL), S. epidermidis (MIC: 16 µg/mL), and C. albicans (MIC: 16 µg/mL) and C. tropicalis (MIC: 32 µg/mL). The results indicate that H. chionophilum, which is an endemic species, and its essential oils can be used as a new and alternative natural agent thanks to its antioxidant and antimicrobial properties.

Keywords

Antioxidant, antimicrobial, Helichrysum chionophilum

Helichrysum chionophilum’un uçucu yağının antioksidan ve antimikrobiyal aktiviteleri: Türkiye'nin endemik bir bitkisi

Abstract

Mikroorganizmalara bağlı olarak gelişen rahatsızlıklar günümüzde giderek tehlikeli bir hal almıştır. Özellikle, dirençli organizmalar ile mücadele noktasında, daha ucuz, doğal ve etkili yöntemlere ihtiyaç duyulmaktadır. Biyolojik olarak aktif bileşikleri barındıran bitki materyalleri bu manada ön plana çıkmaktadır. Bu nedenle, mevcut çalışmada, Gümüşhane'den toplanan Helichrysum chionophilum'dan elde edilen uçucu yağın antioksidan ve antimikrobiyal aktiviteleri araştırılmıştır. Uçucu yağ, Clevenger tipi bir aparat kullanılarak hidro-damıtma yönteminden elde edildi. Serbest radikal yakalama aktivitesi, DPPH (2,2-difenil-1-pikrilhidrazil) yöntemi kullanılarak belirlendi. Antimikrobiyal aktivite mikro dilüsyon yöntemi kullanılarak 17 bakteri ve 2 maya kullanılarak incelendi. Elde edilen bulgulara göre, H. chionophilum bitkisi radikal süpürme aktivitesinin IC50 değeri 260.0±0.2 µg/mL olarak bulunmuştur. Ayrıca, bitkinin uçucu yağı, Y. pseudotuberculosis (MIC: 16 µg/mL), S. epidermidis (MIC: 16 µg/mL) ve C. albicans (MIC: 16 µg/mL) ve C. tropicalis'e (MIC: 32 µg/mL) karşı olağanüstü antimikrobiyal aktivite göstermiştir. Elde edilen sonuçlar, endemik bir tür olan H. chionophilum ve uçucu yağlarının antioksidan ve antimikrobiyal özellikleri sayesinde yeni ve alternatif doğal bir ajan olarak kullanılabileceğini işaret etmektedir.

Keywords

Antioksidan, antimikrobiyal, Helichrysum chionophilum

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