TIBBİ LABORATUVARLARDA KULLANILAN ELİSA VE LC-MS CİHAZLARININ ANALİZ ÖLÇÜMLERİ ÜZERİNDEN KARŞILAŞTIRILMASI

Abstract

Klinik numunelerde yapılan kalitatif ve kantitatif tayinlerin hastaların sağlığı açısından kritik bir öneme sahip olduğu bilinmektedir. Klinik analizlerin gerçekleştirildiği cihazların performans ve kaliteleri, sonuçların doğruluğunu etkilemektedir. Hastalığın tanısının konması ve tedavide doz ayarının yapılabilmesi gibi çok önemli kararların alınabilmesi, cihazların performansına bağlıdır. Bu çalışmada klinik uygulamalar arasından toksikolojik analiz, steroid hormon ölçümü, tam kanda takrolimus seviyesi ve idrarda bisfenol A analizleri için ELİSA (enzim bağlı immunosorbent analizi) ve LC-MS (sıvı kromatografi-tandem kütle spektrometresi) tekniği karşılaştırılmış ve analiz seçimini nasıl etkilediği araştırılmıştır.

Keywords

• ELİSA
• LC-MS
• toksikoloji
• steroid hormon
• LC-MS/MS
• LC-QTOF

References

1. Msagati, T.A.M., Food Forensics and Toxicology. Johannesburg. John Wiley & Sons, 2018.
2. Crowther, J.R., Basic Immunology. In: ELISA pp:1–34. New Jersey. Humana Press, 1995.
3. Cross, T.G., Hornshaw, M.P., Can LC and LC-MS ever replace immunoassays? J Appl Bioanal, 2(4):108–116, 2016.
4. Fuchs, S.M., Krajewski, K., Baker, R.W., Miller, V.L., Strahl, B.D., Influence of Combinatorial Histone Modifications on Antibody and Effector Protein Recognition. Current Biology, 21(1):53–58, 2011.
5. de Hoffmann, E., Stroobant, V., Mass Spectrometry: Principles and Applications. 3rd ed. England. John Wiley & Sons, 2007.
6. Karpievitch, Y.V., Polpitiya, A.D., Anderson, G.A., Smith, R.D., Dabney, A.R., Liquid chromatography mass spectrometry-based proteomics: Biological and technological aspects. Ann Appl Stat, 4(4):1797–1823, 2010.
7. Murphy, R.E., Kinhikar, A.G., Shields, M.J., del Rosario, J., Preston, R., Levin, N., Ward, G., Combined use of immunoassay and two-dimensional liquid chromatography mass spectrometry for the detection and identification of metabolites from biotherapeutic pharmacokinetic samples. J Pharm Biomed Anal, 53(3):221–227, 2010.
8. Piekoszewski W, Florek E., The role of laboratory examinations in medical toxicology. Przegl Lek., 62(10):954-9. PMID: 16521926, 2005.

9. Hemmings, H.C., Egan, T.D. Pharmacology and physiology for anesthesia: foundations and clinical application. Philadelphia. Elsevier/Saunders, 2013.
10. Kennedy, D., Dani, M., Comparative Analysis of ELISA Immunoassay and LC-QTOF for Opiate Screening. J Anal Toxicol, 44(4):410–413, 2020.
11. Frye, C.A., Steroids, reproductive endocrine function, and affect. A review. Minerva Ginecol, 61(6):541–562, 2009.
12. Yucel, K., Abusoglu, S., Unlu, A., Comparison of Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry Methods in the Measurement of Serum Androstenedione Levels. Clin Lab, 64(1):69–75, 2018.
13. Fernández de Castillo Torras, L., Biterna Tejeiro, A., Pla Farnós, M.J., Ponce Sebastià, J., Treatment with ulipristal acetate (Esmya ®) and plasma levels of tacrolimus: a case report. European Journal of Obstetrics & Gynecology and Reproductive Biology, 203:337–338, 2016.
14. Staatz, C.E., Taylor, P.J., Tett, S.E., Comparison of an ELISA and an LC/MS/MS Method for Measuring Tacrolimus Concentrations and Making Dosage Decisions in Transplant Recipients. Ther Drug Monit, 24(5):607–615, 2002.
15. Norman, A.W., From vitamin D to hormone D: fundamentals of the vitamin D endocrine system essential for good health. Am J Clin Nutr, 88(2):491S-499S, 2008.
16. Arneson, W.L., Arneson, D.L., Current Methods for Routine Clinical Laboratory Testing of Vitamin D Levels. Lab Med, 44(1): e38–e42, 2013.
17. Hengstler, J.G., Foth, H., Gebel, T., Kramer, P-J., Lilienblum, W., Schweinfurth, H., Völkel, W., Wollin, K.-M., Gundert-Remy, U., Critical evaluation of key evidence on the human health hazards of exposure to bisphenol A. Crit Rev Toxicol, 41(4):263–291, 2011.
18. Fukata, H., Miyagawa, H., Yamazaki, N., Mori, C., Comparison of Elisa- and LC-MS-Based Methodologies for the Exposure Assessment of Bisphenol A. Toxicol Mech Methods, 16(8):427–430, 2006.
19. Gandhi, A.S., Budac, D., Khayrullina, T., Staal, R., Chandrasena, G., Quantitative analysis of lipids: a higher-throughput LC–MS/MS-based method and its comparison to ELISA. Future Sci OA, 3(1): FSO157, 2017.
20. Gross, M. S., Woodward, E. E., & Hladik, M. L., Evaluation of ELISA for the analysis of imidacloprid in biological matrices: Cross-reactivities, matrix interferences, and comparison to LC-MS/MS. Chemosphere, 286(3): 131746, 2022.
21. Tron, C., Lemaitre, F., Bros, P., Goulvestre, C., Franck, B., Mouton, N., & Blanchet, B., Quantification of infliximab and adalimumab in human plasma by a liquid chromatography tandem mass spectrometry kit and comparison with two ELISA methods. Bioanalysis, 14(11): 831-844, 2022.
22. Foulon, N., Goonatilleke, E., MacCoss, M. J., Emrick, M. A., & Hoofnagle, A. N., Multiplexed Quantification of Insulin and C-peptide by LC-MS/MS without the Use of Antibodies. Journal of Mass Spectrometry and Advances in the Clinical Lab., 25:19-26, 2022.