TIBBİ LABORATUVARLARDA KULLANILAN ELİSA VE LC-MS CİHAZLARININ ANALİZ ÖLÇÜMLERİ ÜZERİNDEN KARŞILAŞTIRILMASI

Year 2022, , 427 - 438, 31.12.2022

Sevilay İnal Kabala , Saffet Çelik

https://doi.org/10.34186/klujes.1204116

Abstract

Klinik numunelerde yapılan kalitatif ve kantitatif tayinlerin hastaların sağlığı açısından kritik bir öneme sahip olduğu bilinmektedir. Klinik analizlerin gerçekleştirildiği cihazların performans ve kaliteleri, sonuçların doğruluğunu etkilemektedir. Hastalığın tanısının konması ve tedavide doz ayarının yapılabilmesi gibi çok önemli kararların alınabilmesi, cihazların performansına bağlıdır. Bu çalışmada klinik uygulamalar arasından toksikolojik analiz, steroid hormon ölçümü, tam kanda takrolimus seviyesi ve idrarda bisfenol A analizleri için ELİSA (enzim bağlı immunosorbent analizi) ve LC-MS (sıvı kromatografi-tandem kütle spektrometresi) tekniği karşılaştırılmış ve analiz seçimini nasıl etkilediği araştırılmıştır.

Keywords

ELİSA, LC-MS, toksikoloji, steroid hormon, LC-MS/MS, LC-QTOF

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