Bellevalia edirnensis ÖZHATAY & MATHEW: Türkiye Endemik Bitkisinin Kallus Kültürünün Anti-Alzheimer ve Anti-diyabetik Aktivitesinin Araştırılması

Abstract

Sekonder metabolitlerin üretimi için alternatif bir teknik olan kallus kültürü, bir bitki doku kültürü yöntemidir. Bu çalışmada, endemik ve nesli tükenme tehlikesi altında olan Edirne sümbülünden (Bellevalia edirnensis ÖZHATAY & MATHEW) elde edilen kallus dokusunun etanol ekstraktlarının anti-alzheimer ve anti-diyabetik aktivitesinin belirlenmesi amaçlanmıştır. Kallus indüksiyonu, tohumların % 20 çamaşır suyu ve % 70 etanol içerisinde sterilize edilmesiyle başladı. Ardından 7 g/L agar ve 30 g/L sükroz katkılı besiyerine ek olarak 3 farklı konsantrasyonda (Murashige ve Skogg) GA3 içeren MS besiyerinde kültüre edilerek elde edildi. Elde edilen kallusların Anti-Alzheimer aktivitesi spektrofotometrik bir yöntem olan Ellman yöntemi kullanılarak belirlendi. Anti-diyabetik aktiviteler, α-glukozidaz inhibisyon yöntemi kullanılarak belirlendi.

Keywords

• Anti-alzheimer aktivite
• Anti-diyabetik aktivite
• Bellevalia edirnensis
• Kallus kültürü

Investigation of Anti-Alzheimer and Anti-Diabetic Activity of Callus Culture of Bellevalia edirnensis ÖZHATAY & MATHEW: An Endemic Plant From Turkey

Abstract

Callus culture, an alternative technique for the production of secondary metabolites, is a plant tissue culture method. In this study, it was aimed to determine the anti-alzheimer and anti-diabetic activity of ethanol extracts of callus tissue obtained from the Edirne hyacinth (Bellevalia edirnensis ÖZHATAY & MATHEW), which is endemic and is in danger of extinction. Callus induction started by sterilization of the seeds in 20% bleach and 70% ethanol. Following, it was achieved by culturing in MS medium containing (Murashige ve Skoog) GA3 in 3 different concentrations in addition to the medium supplemented with 7 g/L agar and 30 g/L sucrose. Anti-Alzheimer activity of the obtained calluses were determined using the Ellman method which is a spectrophotometric method. Anti-diabetic activities were determined using the α-glucosidase inhibition method. According to the results obtained, it was found that the callus culture of Bellevalia edirnensis ÖZHATAY & MATHEW showed moderate Anti-Alzheimer and Anti-diabetic activity.

Keywords

• Anti-azlheimer activity
• Anti-diabetic activity
• Bellevalia edirnensis
• Callus culture

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