DETERMINATION OF ANTIOXIDANT PROPERTIES OF AGARICUS CAMPESTRIS, PLEUROTUS ERYNGII AND LACTARIUS DELICIOSUS

Abstract

Agaricus campestris, Pleurotus eryngii and Lactarius deliciosus are species of fungi that naturally grow in the Thrace region. Mushrooms have been used for food and medical purposes for centuries. In this study, the DPPH scavenging activity, the total phenolic substance contents and the reducing power capacities on the methanol and acetone extracts of the mushroom samples were studied. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest scavenging activity was found in the acetone extract of P. eryngii with 39.86 ± 2.07 %. It was determined that the total phenolic substances in acetone and methanol extracts of mushroom samples varied between 23,25 ± 0,35-28 ± 0,7 μg GAE / mg extract. The sample showing the highest reducing power capacity was an acetone extract of P. eryngii with a concentration of 0,400 ± 0,021 μg/mL at a concentration of 1000 μg/mL followed by the methanol extract of P. eryngii (0.259 ± 0.030) and the acetone extract of L. deliciosus (0.143 ± 0.010).

Keywords

• mushroom,antioxidant

AGARICUS CAMPESTRIS, PLEUROTUS ERYNGII VE LACTARIUS DELICIOSUS MANTARLARININ ANTİOKSİDAN ÖZELLİKLERİNİN BELİRLENMESİ

Abstract

Agaricus campestris , Pleurotus eryngii ve Lactarius deliciosus Trakya bölgesinde doğal olarak yetişebilen mantar türleridir. Mantarlar yüzyıllardır besin kaynağı olarak ve tıbbi amaçla kullanılmaktadırlar. Bu çalışmada mantar örneklerinin metanol ve aseton ekstraktlarının DPPH radikalini süpürme etkileri, toplam fenolik madde miktarları ve indirgeme gücü kapasiteleri çalışılmıştır. DPPH radikalini süpürme etkileri göz önünde bulundurulduğunda konsantrasyona bağlı olarak aktiviteleri artmıştır. En yüksek süpürme etkisi % 39,86± 2,07 ile P. eryngii’nin aseton ekstraktında görülmüştür. Mantar örneklerinin aseton ve metanol ekstraktlarının toplam fenolik madde miktarlarının 23,25±0,35-28±0,7 µg GAE/mg ekstre arasında değiştiği belirlenmiştir. En yüksek indirgeme gücü kapasitesi gösteren örnek 1000 µg/mL’lik konsantrasyonda 0,400±0,021 µg\mL ile P. eryngii’nin aseton ekstraktı olmuştur, bu örneği P. eryngii’nin metanol ekstraktı  (0,259±0,030) ve L. deliciosus’un aseton ekstraktı (0,134±0,010) takip etmektedir.  

Keywords

• Mantar,Antioksidan

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