Toksijenik Aspergillus flavus’un Büyümesini Kontrol Etmek için Thymus longicaulis’in Değerlendirilmesi

Year 2023, Volume: 9 Issue: 2, 443 - 453, 31.12.2023

Bilal Balkan , Seda Balkan

https://doi.org/10.34186/klujes.1314299

Abstract

Bu araştırmada Lamiaceae, familyalasına ait Thymus longicaulis’in sulu ekstraktlarının Aspergillus flavus’ un büyümesi üzerine olan etkisinin in vitro ve in vivo olarak değerlendirilmesi gerçekleştirilmiştir. Kurutulmuş T. longicaulis’in tozları A flavus’ un misel büyümesini %70.53 oranında inhibe etmiştir. T. longicaulis sulu ekstraktlarının A. flavus’a karşı minimum inhibitör konsantrasyon (MİK) değeri 1000 µg/mL idi. T. longicaulis sulu ekstraktları sıvı besiyerindeki 5 günlük saklanma sürelerinden sonra petri kaplarına aktarıldığında ayçiçeği, buğday, mısır ve pirinçte yapay olarak oluşturulan A. flavus çürümesini tamamen engelleyememiştir. Test edilen tüm konsantrasyonlarda 5 günlük inkübasyon süresi boyunca T. longicaulis sulu ekstraktlarının sadece ayçiçeğinde etkin olduğu belirlenmiştir (p<0.05). T. longicaulis sulu ekstraktlarının hifal morfoloji üzerine lizis, çökertme, yassılaşma ve kırışık hücre yüzeyli hücreler dejeneratif değişimlerini yaptığı SEM analizi ile tespit edilmiştir. Araştırmanın sonuçlarına göre T. longicaulis sulu ekstraktlarının aflatoksin B1 üreticisi olan toksijenik A. flavus’un ayçiçeğinde oluşturduğu enfeksiyonları önleyebilmek için koruyucu olarak rol oynayabileceğini ifade edebiliriz.

Keywords

Aspergillus flavus, Thymus longicaulis, Antifungal, Bitki ekstraktı, SEM

Supporting Institution

Kırklareli Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

KLÜBAP/228

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