Bellevalia edirnensis ÖZHATAY & MATHEW: Türkiye Endemik Bitkisinin Kallus Kültürünün Anti-Alzheimer ve Anti-diyabetik Aktivitesinin Araştırılması

Year 2023, Volume: 9 Issue: 2, 281 - 288, 31.12.2023

Burhan Ceylan , Sergun Dayan , Eda Duğan

https://doi.org/10.34186/klujes.1329802

Cited By: 1

Abstract

Sekonder metabolitlerin üretimi için alternatif bir teknik olan kallus kültürü, bir bitki doku kültürü yöntemidir. Bu çalışmada, endemik ve nesli tükenme tehlikesi altında olan Edirne sümbülünden (Bellevalia edirnensis ÖZHATAY & MATHEW) elde edilen kallus dokusunun etanol ekstraktlarının anti-alzheimer ve anti-diyabetik aktivitesinin belirlenmesi amaçlanmıştır. Kallus indüksiyonu, tohumların % 20 çamaşır suyu ve % 70 etanol içerisinde sterilize edilmesiyle başladı. Ardından 7 g/L agar ve 30 g/L sükroz katkılı besiyerine ek olarak 3 farklı konsantrasyonda (Murashige ve Skogg) GA3 içeren MS besiyerinde kültüre edilerek elde edildi. Elde edilen kallusların Anti-Alzheimer aktivitesi spektrofotometrik bir yöntem olan Ellman yöntemi kullanılarak belirlendi. Anti-diyabetik aktiviteler, α-glukozidaz inhibisyon yöntemi kullanılarak belirlendi.

Keywords

Anti-alzheimer aktivite, Anti-diyabetik aktivite, Bellevalia edirnensis, Kallus kültürü

Investigation of Anti-Alzheimer and Anti-Diabetic Activity of Callus Culture of Bellevalia edirnensis ÖZHATAY & MATHEW: An Endemic Plant From Turkey

Abstract

Callus culture, an alternative technique for the production of secondary metabolites, is a plant tissue culture method. In this study, it was aimed to determine the anti-alzheimer and anti-diabetic activity of ethanol extracts of callus tissue obtained from the Edirne hyacinth (Bellevalia edirnensis ÖZHATAY & MATHEW), which is endemic and is in danger of extinction. Callus induction started by sterilization of the seeds in 20% bleach and 70% ethanol. Following, it was achieved by culturing in MS medium containing (Murashige ve Skoog) GA3 in 3 different concentrations in addition to the medium supplemented with 7 g/L agar and 30 g/L sucrose. Anti-Alzheimer activity of the obtained calluses were determined using the Ellman method which is a spectrophotometric method. Anti-diabetic activities were determined using the α-glucosidase inhibition method. According to the results obtained, it was found that the callus culture of Bellevalia edirnensis ÖZHATAY & MATHEW showed moderate Anti-Alzheimer and Anti-diabetic activity.

Keywords

Anti-azlheimer activity, Anti-diabetic activity, Bellevalia edirnensis, Callus culture

References

• Mazid, M., Khan, T., & Mohammad F. (2011). Role of secondary metabolites in defence mechanisms of plants. Biology and Med., 3(2), 232-249.
• Ceylan, B., Tırıs, G. & Kepekci Tekkeli E. (2021). A new HPLC method with UV detection for the determination of carnosol in human plasma and application to a pharmacokinetic study. Chromatoraphia., 89(9), 855-860.
• Elansary, H.O., Szopa, A., Kubica, P., Al-Mana, F.A., Mahmoud, E.A., El-Abedin, T.K.A.Z., Mattar, M.A., & Ekiert, H. (2019). Pheolic compounds of Catalpa speciose Taxus cuspidate, and Magnolia acuminata have antioxidant and anticancer activity. Molecules, 24(3), 412.
• Serim, E., Ceylan, B., & Tekkeli Kepekci S.E. (2023). Determination of apigenin in cosmetics containing chamomile by High-Performance Liquid Chromatography with Ultraviolet Detection (HPLC-UV). Analytical Letters, 56(13), 2113-2122.
• Ceylan, B., & Yeşiloğlu Y. (2022). Investigation of antioxidant activity of Sorbus domestica L. extracts and determination of phenolic contents by LC-MS/MS. Revue Roumaine De Chimie, 67(6-7), 343-351.
• Kaya, G., Ozcan, C. & Yaman, M. (2010). Flame atomic absorption spectrometric determination of Pb, Cd, and Cu in Pinus nigra L. and Eriobotrya japonica leaves used as biomonitors in environmental pollution. Bullutein of Environmental Contamination and Toxicology, 84, 191-196.
• Kaplan, O., Kaya, G., Ozcan, C., Ince, M., & Yaman, M. (2009). Acrylamide concentrations in grilled foodstuffs of Turkish kitchen by high performance liquid chromatography-mass spectrometry. Microchemical Journal, 93(2), 173-179.
• Uzunhisarcıklı, M.E., Duman, H., & Yılmaz, S. (2013). A new species of Bellevalia (Hyacinthaceae) from Turkey, Doga. Turkish Journal Botany, 37(4), 651-655.
• Zhong, J.J. (2001). Biochemical engineering of the production of plant-specific secondary metabolites by cell suspension cultures. Adv. Biochem. Eng. Biotechnol., 72, 1-26.
• Yetim-Kurnaz, N., Hasanoğlu-Özkan, E., Akkurt, N., & Koç, M.M. (2022). Catalytic performance of Fe3O4@g2-Pamam/MoO3 magnetic nanocomposites for degradation of 4-Np to 4-Ap. Journal of Materials and Electronic Devices, 3(1), 1-6.
• Akkurt, N., Hadi-Aljumaili, M., Ocak. H., Cakar, F., & Torun, L. (2020). Synthesis and liquid crystalline properties of new triazine-basedπ-conjugated macromolecules with chiral side groups. Turkish Journal of Chemistry, 44(3), 726-735.
• Ertaş, A., Gören, A.C., Boğa, M., Yeşil, Y., & Kolak, U. (2014). Essential oil compositions and anticholinesterase activities of two edible plants Tragopogon latifolius var. angustifolius and Lycopsis orientalis. Natural Product Research, 28(17), 1405-1408.
• Kurnaz-Yetim, N., Hasanoğlu-Özkan, E., Özcan, C., & Sarı, N. (2020). Preparation of AChE immobilized microspheres containing thiophene and furan for the determination of pesticides by the HPLC-DAD method. Journal of Molecular Structure, 1222, 128931.
• Thilagam, E., Parimaladevi, B., Kumarappan, C., & Mandal, S.C. (2013). α-Glucosidase and α-amylase inhibitory activity of Senna surattensis. J. Acupunct Meridian Stud., 6(1), 24-30.
• Jonhson, M.A.T. (2003). Polyploidy and karyotype variation in Turkish Bellevalia (Hyacinthaceae). Botanical J. Linnean Soc., 143(1), 87-98.
• Dane, F. (1999). Hekzaploid (2n=24) Bellevalia edirnensis Özhatay & Mathew’in polen mitozu ve polen morfolojisinin incelenmesi. Turkish J. Bio., 23(3), 357-368.
• Dane, F. (2006). Cytological and histological studies on reproductive system of hexaploid Bellevalia edirnensis Ozhatay & Mathew (Hyacinthaceae), Acta Biol. Hung., 57(3), 339-354.
• Ellman, G.L., Courtney, K.D. Andress, V.J. & Feather-Stone, R.M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol., 7, 88-95.
• Tsujii, E., Muroi, M., Shiragami, N. & Takatsuki, A. (1996). Nectrisine is a potent inhibitor of alpha-glucosidases, demonstrating activities similarly at enzyme and cellular levels. Biochem. Biophys. Res. Commun., 220(2), 459-466.
• El-Elimat, T., Al-Qiam, R., Burdette, J.E., Sharie, A.H., Al-Gharaibeh, M., & Oberlies, N.H. (2022). Homoisoflavonoids from the bulbs of Bellevalia longipes and assessment of their potential cytotoxic activity. Pyhtochem. 203, 113343.
• Alali, F., El-Elimat, T., Albataineh, H., Al-Balas, Q., Al-Gharaibeh, M., Fankinham, J.O., Chen, W.L., Swanson, S.M., & Oberlies, N.H. (2015). Cytotoxic homoisoflavones from the bulbs of Bellevalia eigii. J. Nat. Product. 78(7), 1708-1715.