PROTECTIVE EFFECT OF HESPERIDIN ON OXIDATIVE STRESS, INFLAMMATION AND APOPTOSIS IN ABAMECTIN-INDUCED GASTRIC TOXICITY

Year 2024, , 30 - 36, 17.01.2024

Sibel Çiğdem Tuncer , Cihan Gür , Hasan Şimşek , Fatih Mehmet Kandemir

https://doi.org/10.18229/kocatepetip.1233652

Abstract

OBJECTIVE: Abamectin is a pesticide type that is widely used in the agricultural industry today and has toxic effects because of long-term exposure. As a result of long-term exposure to abamectin, it causes an increase in oxidative stress-related inflammation and apoptosis. Hesperidin is a flavonoid found naturally in citrus fruits with antioxidant, anti-inflammatory, and anti-apoptotic effects. In this study, it was aimed to investigate the use and possible effects of hesperidin, a natural antioxidant, on abamectin-induced gastric tissue damage.
MATERIAL AND METHODS: 100 or 200 mg/kg (according to the group) hesperidin was administered 30 min before the 1 mg/kg dose of abamectin for 28 days. On the 29th day, 35 animals were decapitated and their stomach tissues were removed and oxidative stress, inflammation, and apoptotic parameters were analyzed by biochemical methods.
RESULTS: Abamectin increased the levels of oxidative stress, inflammation, and apoptosis parameters in gastric tissues compared to the control group (p<0,001). With the administration of hesperidin, all these abamectin-induced increases decreased (p<0,001).
CONCLUSIONS: It was concluded that hesperidin has potential protective properties against the toxic effect on gastric tissues caused by abamectin exposure.

Keywords

Abamectin, Hesperidine, Oxidative stress, Inflammation, Apoptosis

ABAMEKTİN KAYNAKLI MİDE TOKSİSİTESİNDE HESPERİDİNİN OKSİDATİF STRES, İNFLAMASYON VE APOPTOZ ÜZERİNDEKİ KORUYUCU ETKİSİ

Abstract

AMAÇ: Abamektin günümüzde tarım endüstrisinde çok fazla kullanılan bir pestisit türü olup uzun süreli maruziyet sonucunda toksik etkilere sahiptir. Uzun süreli abamektin maruziyeti sonucunda özellikle oksidatif stres ilişkili inflamasyon ve apoptoz artışına neden olmaktadır. Hesperidin, narenciyelerde doğal olarak bulunan antioksidan, antiinflamatuvar ve antiapoptotik etkilere sahip bir flavanoiddir. Bu çalışmada, abamektin kaynaklı mide doku hasarı üzerine doğal bir antioksidan olan hesperidinin kullanımı ve muhtemel etkilerinin araştırılması amaçlanmıştır.
GEREÇ VE YÖNTEM: 28 gün boyunca 1 mg/kg dozda abamektin uygulamasından 30 dakika önce grubuna göre 100 ve 200 mg/kg hesperidin uygulaması yapıldı. 29. günde 35 adet hayvan dekapite edilerek mide dokuları alındı ve biyokimyasal yöntemler ile oksidatif stres, inflamasyon ve apoptotik parametrelerin analizi yapıldı.
BULGULAR: Abamektin mide dokularında oksidatif stres, inflamasyon ve apoptoz parametrelerin düzeylerini kontrol grubuna göre arttırdı (p<0,001). Hesperidin uygulamasıyla birlikte abamektine bağlı tüm bu artış miktarlarında azalmalar meydana gelmiştir (p<0,001).
SONUÇ: Abamektin maruziyetinin neden olduğu mide dokularındaki toksik etkiye karşı hesperidinin potansiyel koruyucu özelliklere sahip olduğu sonucuna varıldı.

Keywords

Abamektin, Hesperidin, Oksidatif stres, İnflamasyon, Apoptoz.

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