APIUM GRAVEOLENS EKSTRAKTLARININ LNCaP HÜCRELERİNDE KASPAZ-3, -8, -9 ve APAF-1 ÜZERİNE ETKİSİ

Year 2024, , 300 - 306, 18.07.2024

Halit Buğra Koca , Tülay Köken , Tülay Akan

https://doi.org/10.18229/kocatepetip.1340255

Abstract

AMAÇ: Bu çalışmada, prostat kanseri hücre hattı LNCaP hücrelerinde, apium graveolens (kereviz) bitkisinin etanolik ekstraktlarının hücre canlılığı ve apoptoziste rol alan kaspazlar ve apoptoz proteaz aktivasyon faktörü-1 (Apaf-1) üzerine indükleyici/baskılayıcı olarak anti-kanser etkisinin araştırılması amaçlandı.
GEREÇ VE YÖNTEM: Çalışmamızda LNCaP hücre hattı kullanıldı. LNCaP hücreleri; 0, 1500, 2000, 2500 μg/mL arasında değişen ve artan konsantrasyonlarda apium graveolens'in etanolik ekstraktları ile muamele edilen 4 gruba ayrıldı. Belirtilen ekstrakt konsantrasyonları LNCaP hücrelerine 24 ve 48 saat ayrı ayrı uygulandı. Uygulama bitiminde hazırlanan hücre lizatından ELISA yöntemiyle; Kaspaz-3, -8, -9 ve Apaf-1 ölçümü yapıldı. Sonuçlar canlı hücre sayılarıyla oranlanarak ng/ml olarak verildi.
BULGULAR: LNCaP prostat kanser hücrelerinin apium graveolens ekstraktları ile 24 ve 48 saatliğine muamelesi kaspaz-3 ve kaspaz-9 düzeylerinde anlamlı bir farklılığa neden olmadı. Bununla birlikte; 24 ve 48 saatlik uygulamada, 2000 ve 2500 μg/ml ekstrakt konsantrasyonlarında kaspaz-8 değerleri kontrol gruplarına göre ve 2500 μg/ml ekstrakt konsantrasyonunda ise Apaf-1 düzeyi kontrol gruplarına göre doza ve zamana bağımlı olarak istatistiksel bakımdan anlamlı artış gösterdi.
SONUÇ: Bu çalışmanın sonuçları, apium graveolens bitkisinin etanolik ekstraktlarının uygulandığı LNCaP prostat kanser hücre hattında kaspaz-8 ve Apaf-1 düzeylerini artırarak antikanser ve apoptotik özelliğe sahip olduğunu göstermektedir. Bulgularımızı desteklemek için daha ileri in-vitro ve in-vivo çalışmalara ihtiyaç vardır.

Keywords

Prostat Kanseri, Kaspazlar, Bitki Ekstreleri, Hücre kültürü

Supporting Institution

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

16.KARİYER.188

EFFECT OF APIUM GRAVEOLENS EXTRACTS ON CASPASE-3, -8, -9 AND APAF-1 IN LNCaP CELLS

Abstract

OBJECTIVE: In this study it was aimed to investigate the anti-cancer effect of ethanolic extracts of apium graveolens (celery) plant on cell viability, caspases involved in apoptosis and apoptosis protease activation factor-1 (Apaf-1) as inducer/suppressor in prostate cancer cell line LNCaP cells.
MATERIAL AND METHODS: LNCaP cell line was used in our study. LNCaP cells were divided into 4 groups treated with ethanolic extracts of apium graveolens at increasing concentrations ranging from 0, 1500, 2000, 2500 μg/mL. The indicated extract concentrations were applied to LNCaP cells separately for 24 and 48 hours. Caspase-3, -8, -9 and Apaf-1 were measured by ELISA method from the cell lysate prepared at the end of the treatment. The results were expressed as ng/ml in proportion to the number of viable cells.
RESULTS: Treatment of LNCaP prostate cancer cells with apium graveolens extracts for 24 and 48 hours did not cause a significant difference in caspase-3 and caspase-9 levels. However, caspase-8 levels were significantly increased at 2000 and 2500 μg/ml extract concentrations and Apaf-1 levels were significantly increased at 2500 μg/ml extract concentration in a dose- and time-dependent manner compared to the control groups at 24 and 48 hours.
CONCLUSIONS: The results of this study show that ethanolic extracts of apium graveolens plant have anticancer and apoptotic properties by increasing caspase-8 and Apaf-1 levels in LNCaP prostate cancer cell line. Further in-vitro and in-vivo studies are needed to support our findings.

Keywords

Prostate Cancer, Caspases, Plant extracts, Cell culture

Project Number

16.KARİYER.188

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