PROTECTIVE EFFECTTS OF DANTROLENE AGAINST SPINAL CORD INJURY INDUCED EARLY OXIDATIVE DAMAGEIN RABBIT BLADDER

Year 2019, , 195 - 200, 27.08.2019

İbrahim Keleş , Mehmet Fatih Bozkurt , Abdurrahman Fatih Fidan , Mustafa Karalar , Soner Çoban , Barış Denk

https://doi.org/10.18229/kocatepetip.586526

Abstract

OBJECTIVE: To investigate the effects of early dantrolene treatment of changes in bladder characteristics after spinal cord injury (SCI).

MATERIAL AND METHODS: We studied the possible protective effects of dantrolene on SCI-induced oxidative damage in bladder tissues of rabbit. We devided 38 rabbits into five groups; the control group (1), spinal cord injury (SCI) (2.group), SCI+dantrolene 0. Hour (3.group), SCI+dantrolene 1. Hour (4.group), SCI+dantrolene 4. Hour (5.group). After a 24 hour euthanasia the urinary bladder was removed for gross, histological and biochemical evaluation. Bladder tissues were investigated by light microscopy. Furthermore blood and tissue malondialdehyde (MDA), glutathione (GSH) and nitric oxide (NO) were also determined.

RESULTS: Significant increase in congestion and edema was observed in the early period spinal trauma (SCI) group compared to control group. In groups 3 and 4, dantrolene was more effective in preventing edema and inflammation, whereas in groups 3, 4 and 5, dantrolene did not reduce congestion. MDA levels and NO levels increased and GSH levels were decreased in SCI group compared to controls. Dantrolene was not effective in all three treatment groups to normalize decreased GSH levels after trauma. It was seen that dantrolene was not effective in all three treatment groups to normalize the decreased GSH level after trauma. Increased levels of MDA after spinal trauma were found to be the lowest in group 5 given at the 4th hour of dantrolene.

CONCLUSIONS: It can be concluded that dantrolene given in the early period is effective in reducing inflammation and edema in the bladder and not effective in decreasing post-traumatic GSH levels to normal. As a result; dantrolene may be helpful in correcting impaired bladder morphology.

Keywords

Spinal cord injury, Dantrolene, Anti-inflammatory, Antioxidant

TAVŞANDA SPİNAL KORD TRAVMASININ NEDEN OLDUĞU ERKEN OXİDATİF STRESE KARŞI DANTROLENİN MESANE ÜZERİNE KORUYUCU ETKİLERİ

Abstract

AMAÇ: Spinal kord travmasından sonra mesanede görülen değişiklikler üzerine erken dönem dantrolen tedavisinin ekinliğinin araştırılması.

GEREÇ VE YÖNTEM: Spinal kord travmasının neden olduğu tavşan mesanesindeki oksidatif hasara karşı dantrolenin erken dönemdeki olası koruyucu etkileri araştırıldı. Toplam 38 hayvan 5 gruba ayrıldı,1. grup kontrol, 2. grup spinal travma (ST), 3. grup ST+dantrolen 0. saat, 4. grup ST+dantrolen 1. Saat ve 5. grup ta ST+dantrolen 4. saat olarak belirlendi. Travmadan 24 saat sonra histopatolojik ve biyokimyasal inceleme yapılmak üzere anestezi altında tavşanların mesaneleri operasyonla çıkartıldı. Mesane dokusu ışık mikroskobunda incelendi. Ayrıca kan ve mesane dokusunda malondialdehid (MDA), glutatyon (GSH) and nitrik oxid (NO) düzeylerine bakıldı.

BULGULAR: Spinal travma sonrası erken dönemde kontrol grubuyla karılaştırıldığında travma oluşturulan grupta ödem ve konjesyonda anlamlı artış vardı. Ödem ve inflamasyonu önlemede 3 ve 4. gruplarda dantrolen daha etkiliydi. Ancak 3, 4 ve 5. gruplarda dantrolenin konjesyonu azaltamadığı görüldü. Kontrol grubuyla karşılaştırıldığında MDA ve NO düzeylerinin yükseldiği GSH seviyesinin ise azaldığı görüldü. Travma sonrası azalan GSH seviyesini normale çekmede dantrolenin her 3 tedavi grubunun etkili olmadığı görüldü. Spinal travma sonrası artan MDA seviyesi 24 saat sonunda 5. grupta en düşük seviyelere indi.

SONUÇ: Erken dönemde verilen dantrolenin mesanede inflamasyon ve ödemi azaltmada etkili olduğu, travma sonrası azalan GSH seviyelerini normale yaklaştırmada ise etkin olmadığı söylenebilir. Sonuç olarak; dantrolen bozulmuş mesane morfolojisini düzeltmede yardımcı

Keywords

Spinal kord travması, dantrolen, anti-inflamatuar

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