KONTRASTLA İNDÜKLENEN NEFROPATİNİN TAVŞAN MODELİNDE CURCUMİNİN KORUYUCU ETKİSİNİN ARAŞTIRILMASI

Year 2022, , 326 - 334, 18.07.2022

Zülfükar Sarıtaş , Hazen Sarıtaş , Musa Korkmaz , Hasan Hüseyin Demirel , Aziz Bülbül , Tuba Berra Sarıtaş , Fatma Görücü

https://doi.org/10.18229/kocatepetip.865037

Abstract

AMAÇ: Tavşanlarda curcuminin kontrast nefropatisi üzerine etkilerinin araştırılması.
GEREÇ VE YÖNTEM: Bu çalışmada 14 yetişkin, 2,5-3 kg beyaz erkek Yeni Zelanda tavşanı rastgele 3 gruba ayrıldı. Gruplar kontrol grubu (n=2), kontrastla indüklenen nefropati grubu (n=6) ve Curcumin grubundan (n=6) oluşturuldu. Curcumin grubunda kontrast madde verilmesinden bir gün önce ve kontrast maddenin verildiği gün 500 mg/kg Curcumin gastrik gavaj ile uygulandı. İopromid kontrast nefropatisini oluşturmak için 30 dakikalık süre boyunca Vena auricularis marginalise bir katater yerleştirilerek 8 g/kg dozda intravenöz olarak enjekte edildi.
BULGULAR: Kontrastla indüklenen nefropati grubunda Miyeloperoksidaz düzeyi 0. Saatte 4,899±0,424ng/ml bulunurken 48 saat sonra anlamlı bir artış (7,467±0.353 ng/ml) gözlendi (p=0,002). Kontrastla indüklenen nefropati grubunda glomerüllerin vakuolizasyonu, tübüler epitel hücrelerinin vakuoler dejenerasyonu, hiyalin silindirleri ve tübül lümeninde tübüler nekroz Curcumin grubuna göre istatistiksel anlamlı olarak yüksekti (P=0,000).
SONUÇ: Bu sonuçlara dayanarak, güçlü bir antioksidan olan Curcuminin 24 ve 48 saat sonra kontrastla indüklenen nefropatiye karşı önemli bir koruyucu etkiye sahip olduğu sonucuna varıldı. Bu nedenle kontrast maddelerin kullanılmasından önce Curcumin uygulanması, seçilmiş vakalarda kontrastla indüklenen nefropatiyi önlemek için yararlı olabilir.

Keywords

Kontrast nefropatisi, Curcumin, Iopramid, Tavşan.

Supporting Institution

Afton Kocatepe Üniversitesi BAP

Project Number

AKU BAPK 17.Kariyer.238

Thanks

Araştırmamıza destek veren Afton Kocatepe Üniversitesi BAP 'e teşekkür ederiz

INVESTIGATION OF THE PROTECTIVE EFFECT OF CURCUMIN IN A RABBIT MODEL OF CONTRAST - INDUCED NEPHROPATHY

Abstract

OBJECTIVE: To evaluate the effects of curcumin on contrast nephropathy in rabbits.
MATERIAL AND METHODS: In this study, 14 adult, 2.5-3 kg white male New Zealand rabbits were randomly divided into 3 groups. Goups consisted of the control group (n=2) consisted of the contrast-induced nephropathy group (n=6) and the Curcumin group (n=6). In the curcumin group, curcumin was administered via gastric gavage at a dose of 500 mg/kg one day before and on the day of contrast agent administration. Iopromide was injected intravenously at a dose of 8 g/kg via a catheter in the V. auricularis marginalis over a period of 30 minutes at a slow rate to induce contrast nephropathy.
RESULTS: Myeloperoxidase was 4,899 ± 0,424 ng/ml at hour 0 in the contrast-induced nephropathy group and a significant increase was observed after 48 hours (7.467 ± 0.353 ng/mL) (p=0.002). In the contrast-induced nephropathy group, vacuolization of the glomeruli, vacuolar degeneration of the tubular epithelial cells, hyaline casts, necrotic tubular epithelial cells in the tubules was statistically higher compared to the curcumin groups (P=0.000).
CONCLUSIONS: Based upon these results, it was concluded that curcumin, which is a strong antioxidant, had a significant protective effect against contrast-induced nephropathy after 24 and 48 hours. Therefore, the administration of curcumin before the contrast material administration may be beneficial to prevent nephropathy in selected cases

Keywords

Contrast induced nephropathy, Curcumin, Iopromid, Rabbit.

Project Number

AKU BAPK 17.Kariyer.238

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