Telomerase Activity And Sialic Acid Levels In Human Glioma Cell Lines

Year 2007, Volume: 8 Issue: 2, 19 - 24, 01.08.2007

Didem Cosan Ayse Basaran

Abstract

Keywords

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Insan Glioma Hücre Dizilerinde Telomeraz Aktivitesi ve Siyalik Asit

Abstract

Amaç: Telomeraz enzim kompleksi, diğer proteinlerin yanında, TERT ve TR olmak üzere iki temel subunitten oluşur. Polisiyalik asit zincirleri muhtemelen hücrelerin birbirlerine daha kolay yapışmasını sağlayarak, NCAM moleküllünün adeziv fonksiyonunu düzenler. Telomeraz aktivitesi ve siyalik asit düzeyleri gliomaların biyolojik davranışlarını belirlemede kullanılabilir. Gereç ve Yöntem: İnsan glioma hücre dizilerinden elde edilen hücre ekstraktlarında, telomeraz aktivitesi ve siyalik asit düzeyleri araştırıldı. Telomeraz aktivitesini belirlemek için, telomerik tekrar amplifikasyon protokolü uygulandı. Siyalik asit düzeylerini ölçmek için, siyalik asit kiti kullanıldı. Hücre dizisinde hTERT, hTR ve GADPH ekspresyonları RT-PCR kiti kullanılarak belirlendi. NCAM için, flask tabanında fikse edilen hücrelerde streptavidin-biotin peroksidaz boyama metodu uygulandı. Bulgular: İnsan glioma hücre dizilerinin sonraki pasajlarında telomeraz aktivitesi her ne kadar dalgalı olsada artmış olarak bulundu. Telomeraz RNA’sı ve TERT ekspresyonları tüm pasajlarda pozitifti. Siyalik asit düzeyleri de artış gösterdi ve bu artış insan glioma hücre dizilerinin ilerleyen pasajlarında düzenliydi. NCAM molekülü tüm pasajlarda pozitif olarak bulundu. Sonuç: Başlangıçta ilerleyen pasajlarda artması beklenen telomeraz aktivitesi dalgalı olarak artmıştır. Bunun yanında glioma hücre dizilerinin ilerleyen pasajlarında siyalik asit düzeyleri daha düzenli artış göstermektedir

Keywords

DK-MG hücre dizisi, siyalik asit, telomeraz aktivitesi, hTERT, hTR, NCAM

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