Hepatokarsinogenezis'in Erken Doneminde Demetoksiviridin ve 1-a-Hidroksi -demetoksiviridinin Antioksidan Savunma Sistemine Etkisi
Abstract
Keywords
References
- Kuroda H, Ohtsuru A, Futakuchı M, Kawashıta Y, Nagayama Y, Fukuda E, Namba H, Shıraı T, Kanematsu T, Yamashita S. Distinctive Gene Expression of Receptor-type Tyrosine kinase Families during Rat Hepatocarcinogenesis. Int J of Mol Med, 2002; 9: 473-480.
- Thirunavukkarasu C, Sakthisekaran D. Effect of Dietary Selenite on N-Nitrosodiethylamine-Induced And Phenobarbital Promoted Multistage Hepatocarcinogenesis in Rat: Reşection in Some Minerals. Biomedicine & Pharmacotherapy, 2003; : 416—421.
- Wood A, Sarma D, Archer M. Inheritance of Resistance to Promotion of Preneoplastic Liver Lesions in Copenhagen Rats. Exp Biol Med, 2001; (9): 831-835.
- Ahn B, Han B, Kim D, Ohshima H. Immunohistochemical Localization of Inducible Nitric Oxide Synthase and 3-Nitrotyrosine in Rat Liver Tumors Induced by N-Nitrosodiethylamine. Carcinogenesis, 1999; 20(7): 1337-1344.
- Barbason H, Mormont C, Massaıt S, Bouzahzah B. Anti-Carcinogenic Action of Phenobarbital Given Simultaneously with Diethylnitrosamine in The Rat. Eur T Cancer Clin Oncol, 1986; 22(9): 1073—1078.
- Mitsumori K, Onodera H, Shoda T, Uneyama C, Imazawa T, Takegawa K, Yasuhara K, Watanabe T, Takahashi M. Liver Tumuour-promoting Effects of Oxfendazole in Rats. Food Chem. Toxicol, 1997; 35: 806.
- Thullberg M, Kraupp G.B, Högberg J, Garberg P. Changes in Liver Fatty Acid-Binding Protein in Rat Enzyme-Altered Foci. Cancer letters, 1998; 128:1-10.
- Arora A, Shukla Y. Induction of Preneoplastic Altered Hepatic Foci Following Dietary Sulphur Supplementation. Human & Experimental Toxicology, 2004; 23: 229-234.
- Barbisan L, Scolastici C, Miyamoto M, Salvodari D, Ribeiro L, Eira A, Camargo ]. Effects of Crude Extracts of Agaricus Blazei on DNA Damage and on Rat Liver Carcinogenesis Induced by Diethylnitrosamine. Genet Mol Res, 2003; 2(3): 295
- Barbisan S, Kaneno R, Rodrigues M, Salvadori D, Moreira E, Barbisan L, Camargo J. Lymphoproliferative Response and T Lymphocyte Subsets in a Medium-Term Multi-Organ Bioassay for Carcinogenesis in Wistar rats. Cancer Letters, 2000; : 121—129.
- Liu L, Gong L, Qi X, Cai Y, Wang H, Wu X, Xiao Y, Ren J. Altered Expression of Cytochrome P450 and Possible Correlation with Preneoplastic Changes in Das S. Garlic- A Natural Source of Cancer Preventive Compounds. Asian Pacific J. Cancer Prev, 2002; 3:
- Kıran I, Ilhan S, Akar T, Tur L, Erol E. Synthesis and Evaluation of Demethoxyviridin Derivates as Pendl G, Prieschl E, Thumb W, Harrer N, Auer M, Baumruker T. Effects of Phosphatidylinositol-3— Kinase Inhibitors on Degranulation and Gene Induction in Allergically Triggered Mouse Mast Cells. Int Arch Allergy Immunol, 1997; 112: 392
- Kocatepe Tip Dergisi. Cilt 7 No: 3. Eylül 2006.
Hepatokarsinogenezis'in Erken Doneminde Demetoksiviridin ve l-a-Hidroksi -demetoksiviridinin Antioksidan Savunma Sistemine Etkisi
Abstract
Bu çalışmada, 2 aylık erkek Spraque—Dawley türü sı— çanlarda hepatokarsinogenezisin erken safhasında demetoksiviridin (DMV) ve ondan türetilmiş l-a-hidroksidemetoksiviridin (1—0t-hidroksi—DMV)’nin antioksidan sistemine düzeltici etkileri araştırıldı. Bu amaçla deney hayvanları lO gruba ayrıldı. Gruplar, kontrol (l.grup), yağ (2.grup), Dimetil sülfoksit (DMSO) (3.gnıp), DMV (4.grup), l-oıhidroksi—DMV (5.grup), dietilnitrozamine (DEN) (6.grup), 2asetilaminoşoran (2-AAF) (7.grup), DEN+2-AAF (8.grup), DEN+2-AAF+DMV (9.grup) ve DEN+2-AAF+l-0t— hidroksi-DMV olarak düzenlendi. Deneyin 5. haftasında, eter anestezisi altında sıçanların karaciğeri alındı. Karaciğer homojenatları hazırlanarak, süperoksit dismutaz (SOD) ve glutatyon peroksidaz (pr) ile katalaz enzim aktiviteleri ve malondialdehit (MDA) düzeyleri belirlendi. Karaciğer doku preperatlan H&E boyama yöntemi ile histopatolojik olarak değerlendirildi. Elde edilen tüm bulgularda kontrole göre tümör oluşturucu ajanlar dışındaki gruplarda fark görülmezken, DEN+2— AAF verilen grupta daha önemli düzeyde olmak üzere DEN, 2-AAF verilen gruplarda farklılıklar gözlendi. Düzeltici etkisi olduğunu düşündüğümüz DMV ve l-othidroksi-DMV ise MDA düzeyi dışında SOD % inhibisyonu, pr ve katalaz aktivitelerini artırarak, düzeltici etkisi olduğu belirlenirken histolojik olarak da hepatosit karyomegali, hepatosit sitomegali, iltihabi hücre infiltrasyonu, nekroz ve prenoplastik fokusların oluşumunu engellediği ortaya kondu. Sonuç olarak, her iki mantar metabolitinin de preneoplastik dönemde antioksidan sistemi düzeltici etkisi ortaya kondu, Fakat bu sonucu n daha da kesinlesebilrnesi ve preneopla stik donemi takip eden evrelerde antioksidan sisteme ne gibi etkilerinin olabileceg ini ortaya koya bilmek
icin, heniiz cok yeni olan bu konuda , daha cok cahsmaya ihtiyac vardir.
References
- Kuroda H, Ohtsuru A, Futakuchı M, Kawashıta Y, Nagayama Y, Fukuda E, Namba H, Shıraı T, Kanematsu T, Yamashita S. Distinctive Gene Expression of Receptor-type Tyrosine kinase Families during Rat Hepatocarcinogenesis. Int J of Mol Med, 2002; 9: 473-480.
- Thirunavukkarasu C, Sakthisekaran D. Effect of Dietary Selenite on N-Nitrosodiethylamine-Induced And Phenobarbital Promoted Multistage Hepatocarcinogenesis in Rat: Reşection in Some Minerals. Biomedicine & Pharmacotherapy, 2003; : 416—421.
- Wood A, Sarma D, Archer M. Inheritance of Resistance to Promotion of Preneoplastic Liver Lesions in Copenhagen Rats. Exp Biol Med, 2001; (9): 831-835.
- Ahn B, Han B, Kim D, Ohshima H. Immunohistochemical Localization of Inducible Nitric Oxide Synthase and 3-Nitrotyrosine in Rat Liver Tumors Induced by N-Nitrosodiethylamine. Carcinogenesis, 1999; 20(7): 1337-1344.
- Barbason H, Mormont C, Massaıt S, Bouzahzah B. Anti-Carcinogenic Action of Phenobarbital Given Simultaneously with Diethylnitrosamine in The Rat. Eur T Cancer Clin Oncol, 1986; 22(9): 1073—1078.
- Mitsumori K, Onodera H, Shoda T, Uneyama C, Imazawa T, Takegawa K, Yasuhara K, Watanabe T, Takahashi M. Liver Tumuour-promoting Effects of Oxfendazole in Rats. Food Chem. Toxicol, 1997; 35: 806.
- Thullberg M, Kraupp G.B, Högberg J, Garberg P. Changes in Liver Fatty Acid-Binding Protein in Rat Enzyme-Altered Foci. Cancer letters, 1998; 128:1-10.
- Arora A, Shukla Y. Induction of Preneoplastic Altered Hepatic Foci Following Dietary Sulphur Supplementation. Human & Experimental Toxicology, 2004; 23: 229-234.
- Barbisan L, Scolastici C, Miyamoto M, Salvodari D, Ribeiro L, Eira A, Camargo ]. Effects of Crude Extracts of Agaricus Blazei on DNA Damage and on Rat Liver Carcinogenesis Induced by Diethylnitrosamine. Genet Mol Res, 2003; 2(3): 295
- Barbisan S, Kaneno R, Rodrigues M, Salvadori D, Moreira E, Barbisan L, Camargo J. Lymphoproliferative Response and T Lymphocyte Subsets in a Medium-Term Multi-Organ Bioassay for Carcinogenesis in Wistar rats. Cancer Letters, 2000; : 121—129.
- Liu L, Gong L, Qi X, Cai Y, Wang H, Wu X, Xiao Y, Ren J. Altered Expression of Cytochrome P450 and Possible Correlation with Preneoplastic Changes in Das S. Garlic- A Natural Source of Cancer Preventive Compounds. Asian Pacific J. Cancer Prev, 2002; 3:
- Kıran I, Ilhan S, Akar T, Tur L, Erol E. Synthesis and Evaluation of Demethoxyviridin Derivates as Pendl G, Prieschl E, Thumb W, Harrer N, Auer M, Baumruker T. Effects of Phosphatidylinositol-3— Kinase Inhibitors on Degranulation and Gene Induction in Allergically Triggered Mouse Mast Cells. Int Arch Allergy Immunol, 1997; 112: 392
- Kocatepe Tip Dergisi. Cilt 7 No: 3. Eylül 2006.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | December 1, 2006 |
| Published in Issue | Year 2006 Volume: 7 Issue: 3 |
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