KADMİYUM TOKSİSİTESİ OLUŞTURULAN SIÇANLARDA TANNİK ASİTİN, AĞIR METAL GİDERİMİ VE BAZI BİYOKİMYASAL DEĞERLER ÜZERİNE ETKİSİNİN ARAŞTIRILMASI

Year 2017, Volume: 18 Issue: 4, 146 - 153, 16.10.2017

Didem Turgut Coşan Aylin Dal Ahu Soyocak Ertuğrul Çolak Arzu Çiçek Hülyam Kurt

https://doi.org/10.18229/kocatepetip.368688

Cited By: 2

Abstract

AMAÇ: Endüstriyel alanda yoğun bir şekilde kullanılan, önemli
çevre kirleticilerden biri olan kadmiyum (Cd), günlük hayattaki
maruziyetimiz nedeniyle yaşamsal bir takım tehditlere sebep
olabilmektedir. Uzun bir yarılanma ömrüne sahip olan Cd, solunum,
üriner, kardiovasküler, gastrointestinal, sinir sistemi ve kemiklerde
direkt veya dolaylı olarak toksisiteye neden olmaktadır.
Vücutta biriken Cd’un osteoporoz, anemi, eozinofili, anozmi
ve kronik rinit gibi birçok hastalığa neden olduğu bilinmektedir.
Çalışmamızda, flavonoid içeriği oldukça yüksek, ağır metal şelatlayıcı
özelliğe sahip ve antioksidan tannik asidin (TA) Cd toksikasyonundan
korunmadaki rolünü araştırmak amaçlandı.

GEREÇ VE YÖNTEM: Bu amaçla, 28 adet 200-300 gram
ağırlığında sağlıklı 3-4 aylık Spraque-Dawley soyu erkek sıçanlar
kullanıldı. Deney hayvanlarına ön tedavi amaçlı intraperitonal
(i.p) enjeksiyon ile 5 gün boyunca TA 50mg/kg/gün olarak
uygulandıktan sonra, intragastrik (i.g) enjeksiyon tek doz 35mg/
kg CdCl2 uygulandı.

BULGULAR: CdCl2 uygulaması ile azalan hemolizat, karaciğer
ve böbrek süperoksit dismutaz (SOD) değerlerini TA’nın artırdığı
gözlendi. Benzer şekilde artan hemolizat ve karaciğer malondialdehit
(MDA) değerlerini TA’nın azalttığı belirlendi. Karaciğer,
böbrek, akciğer, testis ve dalakta CdCl2 uygulaması ile artan
birikimi azaltma yönünde, TA etkili olmazken, kalpteki birikimi
azaltma yönünde TA’nın etkili olduğu analiz edildi. CdCl2 uygulaması
ile artan demir birikimini azaltma yönünde böbrek, dalak
ve kalpte TA etkili olmazken, karaciğer, akciğer ve testiste etkili
olduğu tespit edildi.

SONUÇ: Günlük diyette kullanılan yiyecek ve içeceklerin, toksik
ajanlara maruz kalma sonucu gelişebilecek bazı hastalıkları
erken dönemde önlenmesiyle, bireylerin yaşam kalitesi yükselebilecektir.
Çalışmamızda günlük yaşantımızda tüketilen
TA içeren yiyeceklerin, çeşitli şekillerde maruz kaldığımız ağır
metallerden korunma üzerine olan etkileri gözlemlendi. Elde
edilen sonuçlara göre farklı dokularda farklı sonuçlara ulaşılabileceği
belirlenirken, genel olarak değerlendirildiğinde, Cd’un
zararlı etkilerine ya da en azından bazı dokulardaki birikimini
azaltma yönünde TA’nın etkili olabileceği sonucuna ulaşıldı.

Keywords

Kadmiyum, Tannik asit, Toksisite

THE INVESTIGATION OF TANNIC ACID EFFECTS ON REMOVAL OF HEAVY METALS AND SOME BIOCHEMICAL VALUES IN THE RAT TOXICITY INDUCED BY CADMIUM

Abstract

OBJECTIVE: Cadmium (Cd), one of the major environmental
pollutants used intensively in the industrial field and could causes
a variety of vital threats due to our exposure in our daily
lives. Having a long half-life, Cd causes direct or indirect toxicity
to respiratory, urinary, cardiovascular, gastrointestinal, nervous
system and bones. It is known that Cd that accumulates in the
body causes many diseases such as osteoporosis, anemia, eosinophilia,
anosmia and chronic rhinitis. In our study, we aimed
to investigate the role of tannic acid (TA), an antioxidant substance
with a high content of flavonoids and heavy metal chelating
agents, in order to investigate its role in protecting from
Cd toxicity.

MATERIALS AND METHODS: In this study, it was used 28
Spraque-Dawley, 200-300 gr, 3-4 monthly male rats. For pre-treatment,
TA (50 mg/kg/day) was administered by intraperitoneal
(i.p) injection to the experimental groups for 5 days. And then
a single dose CdCl2 (35 mg/kg) was was administered by intragastric
(i.g).

RESULTS: It was observed that superoxide dismutase (SOD)
values decreased in hemolysate, liver and kidney by CdCl2 administration;
but increased with TA. Malondialdehyde (MDA)
values increased with CdCl2 administration whereas decreased
with TA the hemolysate and liver. TA was found to be effective
in decreasing heart accumulation, while TA was not effective
in reducing accumulation with CdCl2 administration in liver,
kidney, lung, testis and spleen. TA is not effective in reducing
increased iron accumulation with CdCl2 administration in the
kidney, spleen and heart, but in liver, lung and testis it could
be effective.

CONCLUSIONS: Food and drinks used in the daily diet may
increase the quality of life of individuals by early prevention of
certain diseases that may result in exposure to toxic agents. In
our study, it has been shown that TA, which is consumed in our
daily lives, may be effective in protecting substances from heavy
metal toxicity. According to the results obtained, different
results could be reached in different tissues; but when assessed
in general, the harmful effects of Cd, or at least the result that
TA have been achieved to reduce the accumulation of some tissues.

Keywords

Cadmium, Tannic Acid, Toxicity

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