PROTECTIVE AND THERAPEUTIC EFFECTS OF OLEIC ACID IN EXPERIMENTAL MYOGLOBİNURIC ACUTE RENAL FAILURE MODEL

Year 2025, Volume: 26 Issue: 4, 377 - 385, 13.10.2025

Ferhat Şirinyildiz , Murat Arı , Serdal Öğüt

https://doi.org/10.18229/kocatepetip.1678070

Abstract

OBJECTIVE: This study aims to investigate the potential protective and therapeutic effects of oleic acid recognized for its antioxidant and anti-inflammatory properties on acute renal failure (ARF) in an experimental model.
MATERIAL AND METHODS: In this study, 24 Wistar Albino female rats were randomly divided into 3 groups of 8 each. Group 1 was used as the control group. Group 2 was given 50% hypertonic glycerol (8 ml/kg, i.m.) to induce renal injury. Group 3, glycerol plus oleic acid (10 mg/kg, intragastrically) respectively. Renal injury was assessed by measuring serum urea and creatinine. Biochemical markers malondialdehyde (MDA), total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), total thiol, catalase (CAT), paraoxanase (PON) values were measured to evaluate the balance between oxidative stress and antioxidant defense systems.
RESULTS: Glycerol administration caused a significant increase in serum creatinine and urea levels, indicating acute renal dysfunction. Oleic acid treatment provided partial improvement in these parameters indicating renal function. When we look at biochemical parameters, MDA, TOS and OSI levels reflecting oxidative stress were significantly increased in glycerol-administered rats, while TAS, total thiol, CAT and PON levels were significantly decreased. Oleic supplementation significantly reversed these changes, showing antioxidant and nephroprotective effects.
CONCLUSIONS: The results obtained provide new insights into the potential nephroprotective effects of oleic acid on the kidney and clearly demonstrate the need for further studies to elucidate the mechanisms underlying this effect and to evaluate its clinical applicability.

Keywords

Glycerol , Myoglobinuric acut renal failure , Oleic acid , Free radicals , Oxidative stress.

Ethical Statement

Ethics: Prior to the commencement of the study, approval was granted by the Aydin Adnan Menderes University Faculty of Medicine Experimental Animals Ethics Committee (decision numbered 64583101/2025/056) in accordance with the ethical standards set out in the committee's guidelines.

DENEYSEL MİYOGLOBİNÜRİK AKUT BÖBREK YETMEZLİĞİ MODELİNDE OLEİK ASİDİN KORUYUCU VE TERAPÖTİK ETKİLERİ

Abstract

AMAÇ: Bu çalışmanın amacı, antioksidan ve antiinflamatuar özellikleriyle bilinen oleik asidin, akut böbrek yetmezliği (ABY) üzerindeki potansiyel koruyucu ve tedavi edici etkilerini deneysel bir modelde incelemektir.
GEREÇ VE YÖNTEM: Bu çalışmada 24 adet Wistar Albino dişi sıçan randomize 8’erli 3 gruba ayrıldı. Grup 1 kontrol grubu olarak kullanıldı. Grup 2'ye böbrek hasarını indüklemek için %50 hipertonik gliserol (8 ml/kg, i.m.) verildi. Grup 3'e gliserol ve oleik asit (10 mg/kg, intragastrik) verildi. Böbrek hasarı, serum üre ve kreatinin değerleri ölçülerek değerlendirildi. Oksidatif stres ile antioksidan savunma sistemleri arasındaki dengeyi değerlendirmek amacıyla biyokimyasal belirteçler olan malondialdehit (MDA), total oksidan kapasite (TOS), total antioksidan kapasite (TAS), oksidatif stres indeks (OSI), total thiol, katalaz (CAT), paraoksonaz (PON) değerleri ölçüldü.
BULGULAR: Gliserol uygulaması, serum kreatinin ve üre seviyelerinde anlamlı bir artışa neden oldu ve bu da akut böbrek fonksiyon bozukluğunu gösterdi. Oleik asit tedavisi renal fonksiyonları gösteren bu parametrelerde kısmi iyileşme sağladı. Biyokimyasal parametrelere baktığımızda, gliserol uygulanan sıçanlarda oksidatif stresi yansıtan MDA, TOS ve OSI seviyeleri anlamlı derecede artış gözlemlenirken, TAS, total tiyol, CAT ve PON seviyeleri anlamlı seviyede azaldığı gözlemlendi. Oleik takviyesi, bu değişiklikleri önemli ölçüde tersine çevirerek antioksidan ve nefroprotektif etkiler göstermiştir.
SONUÇ: Elde edilen sonuçlar, oleik asidin böbrek üzerindeki potansiyel nefroprotektif etkilerine dair yeni bakış açıları sunmakta olup, bu etkinin altında yatan mekanizmaların aydınlatılması ve klinik uygulanabilirliğinin değerlendirilmesi amacıyla ileri düzey araştırmalara duyulan gereksinimi açıkça ortaya koymaktadır.

Keywords

Gliserol , Miyoglobinürik akut böbrek yetmezliği , Oleik asit , Serbest radikaller , Oksidatif stres

Ethical Statement

Etik: Çalışmaya başlamadan önce Aydın Adnan Menderes Üniversitesi Tıp Fakültesi Deney Hayvanları Etik Kurulu’nun 64583101/2025/056 sayılı kararıyla etik kurul kurallarına uygun olarak çalışma onayı alındı.

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