BibTex RIS Cite

Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese

Year 2015, Volume: 18 Issue: 1, 31 - 34, 02.11.2015

Abstract

ABSTRACT: This study was aimed to investigate the presence of Escherichia coli O157:H7 in Duhok cheese by culture and Polymerase Chain Reaction (PCR) technique. The cheese samples (n:120) were purchased from different supermarkets and shops from Zakho, Akre, Amedy and Duhok cities during September 2011 to February 2012.

X-gal positive, MUG negative, indole positive colonies were found as 10% (12/120) of samples. These colonies were confirmed by biochemical tests (API E 20 test, latex agglutination tests) and results showed that 7 (5.83%) of E. coli isolates from cheese were positive for O157:H7. These isolates were also confirmed by PCR assay using specific primers for rfbO157 and flicH7. The numbers of positive isolates for rfbO157 and flicH7 were 3.33 and 0.83%, respectively. The PCR results confirmed as 0.83% of samples were E. coli O157:H7.

Key words: Duhok cheese, Escherichia coli O157:H7, Cultural technique, PCR technique

 

Kültür ve PZR Yöntemi ile Duhok Peynirinde Escherichia coli O157:H7'nin Araştırılması

 

ÖZET: Bu çalışmada Duhok peynirinde of E. coli O157:H7'nin varlığı kültür ve Polimeraz Zincir Reaksiyonu tekniği (PZR) ile araştırılmıştır. Peynir örnekleri (n:120) Zakho, Akre, Amedy ve Duhok şehirlerinden Eylül 2011-Şubat 2012 tarihleri arasında yerel market ve bakkallardan temin edilmiştir.

X-gal pozitif, MUG negatif,  indol pozitif olan %10 (12/120) oranında şüpheli koloni tespit edilmiştir. Şüpheli kolonilere biyokimyasal testler uygulanmış (API E 20 ve latex aglütinasyon testi) ve 7 (%5,83) örnekte E. coli O157:H7 tespit edilmiştir. Bu izolatlararfbO157 ve flicH7 primerleri kullanılarak PCR yöntemi ile doğrulama işlemi uygulanmıştır. İzolatlar %3,33 oranında O157 ve 0,83 oranında H7 antijeni pozitif olarak tespit edilmiştir. PCR analizlerinin sonunda örneklerin %0,83'ünün E. coli O157:H7 olduğu gözlenmiştir.

Anahtar Kelimeler: Duhok Peyniri, Escherichia coli O157:H7, Kültür tekniği, PZR tekniği

References

  • Abdul-Raouf, U.M., Ammar, M.S., Beuchat, L.R. 1996. Isolation of Escherichia coli O157:H7 from some Egyptian foods. International Journal of Food Microbiology, 29: 423-426.
  • Altekruse, S.F., Timbo, B.B., Mowbray, J.C., Bean, N.H., outbreaks of human illness in the United States, 1972 to 1992: Sanitary manufacturing practices protect consumers. Journal of Food Protection, 61:1405-1407. 1998. Cheese-associated
  • Andreoli, S.P., Trachtman, H., Acheson, D.W.K., Siegler, R.L., Obrig, T.G. 2002. Hemolytic uremic syndrome: epidemiology, pathophysiology, and therapy. Pediatric Nephrology, 17: 293-297.
  • Beutin, L., Krause, G., Zimmermann, S., Kaulfuss, S., Gleier, K. 2004. Characterization of Shiga toxin- producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. Journal ofClinical Microbiology, 42: 1099-1108.
  • Blanco, J., Blanco, M., Blanco, J.E., Mora, A., González, E..A., Bernárdez, M.I., Alonso, M.P., Coira, A., Rodríguez, A., Rey, J., Alonso, J.M., Usera, M.A. 2003. Verotoxin-Producing Escherichia coli in Spain: Prevalence, Serotypes, and Virulence Genes of O157:H7 and non-O157 VTEC in Ruminants, Raw Beef Products, and Humans. Experimental Biology and Medicine, 228: 345-351.
  • Centers for Disease Control and Prevention (CDC). 2007 Escherichia coli O157:H7 infection associated with drinking raw milk- Washington and Oregon, November December 2005. Morbidity Mortality Weekly Report, 56: 165-167. Weagant, Feng, P., S.D., Jinneman, K. 2011. Diarrheagenic Escherichia coli. FDA-BAM.
  • Ferens, W.A., Hovde, C.J. 2011. Escherichia coli O157:H7: Animal Reservoir and Sources of Human Infection. Foodborne Pathogens and Disease, 8: 465-487.
  • Gannon, V.P.J., D’souza, S., Graham, T., King, R.K., Rahn, K., Read, S. 1997. Use of flagellar H7 gene as a target in multiplex PCR assay and improved specificity and identification of enterohaemorrhagic Escherichia coli Microbiology, 35: 656-662. Journal of Clinical
  • Govaris, A., Koidis, P., Papatheodorou, K. 2002. Behavior of Escherichia coli O157:H7 in sour milk, cow’s milk yogurt and ewe’s milk yogurt. Journal of Dairy Research, 69: 655-660.
  • Honish, L., Predy, G., Hislop, N., Chui, L., Kowalewska-Grochowska, K., Trottier, L., Kreplin, C., Zazulak, I. 2005. An outbreak of Escherichia coli O157:H7 hemorrhagic colitis associated with unpasteurized Gouda cheese. Canadian Journal of Public Health, 96: 182-184.
  • Karmali, M.A. 1989. Infection by verotoxin-producing Escherichia coli. Clinical Microbiology Reviews, 2: 15-38.
  • Morgan, D., Newman, C.P., Hutchinson, D.N., Walker, A.M., Rowe, B., Majid, F. 1993.Verotoxin producing associated with the consumption of yoghurt. Epidemiology and Infection, 111: 181-187.
  • Öksüz, Ö., Arici, M., Kurultay, S., Gümüş, T. 2004. Incidence of Escherichia coli O157 in raw milk and white pickled cheese manufactured from raw milk in Turkey. Food Control, 15, 453-456.
  • Paton, A.W., Paton, J.C. 1998. Detection and Characterization of Shiga Toxigenic Escherichia coli by Using Multiplex PCR Assays for stx1, stx2, eaeA, EnterohemorrhagicE. colihlyA, rfbO111 and rfbO157. Journal of Clinical Microbiology, 36(2): 598-602.
  • Rahimi, E., Chaleshtori, S.S., Parsaei, P. 2011. Prevalence Escherichia coli O157 isolated from traditional cheese, ice creamand yoghurt in Iran. African Journal of Microbiology Research, 5(22): 3706- 3710. resistance of
  • Schlesser, J.E., Gerdes, R., Ravishankar, S., Madsen, K., Mowbray, J., Teo, A.Y.L. 2006. Survival of a five-strain cocktail of Escherichia coli O157:H7 during a 60 day aging period of cheddar cheese made from unpasteurized milk. Journal of Food Protection, 69: 90-99.
  • Schrade, J.P., Yager, J. 2001. Implication of milk and milk products in food disease in France and in different industrialized countries. International Journal of Food Microbiology, 67: 1-17.
  • Stampi, S., Caprioli, A., De Luca, G., Quaglio, P., Sacchetti, R., Zanetti, F. Escherichia coli O157 in bovine meat products in Northern Microbiology, 90: 257-262. 2004. Detection of
  • Italy. International Journal of Food
  • Tobias, J., Vutukuru, S.R. 2012. Simple and rapid multiplex PCR for identification of the main human diarrheagenicEscherichia Research. 167 (9):564-570. coli. Microbiological

-

Year 2015, Volume: 18 Issue: 1, 31 - 34, 02.11.2015

Abstract

Bu çalışmada Duhok peynirinde of E. coli O157:H7'nin varlığı kültür ve Polimeraz Zincir Reaksiyonutekniği (PZR) ile araştırılmıştır. Peynir örnekleri (n:120) Zakho, Akre, Amedy ve Duhok şehirlerinden Eylül 2011Şubat 2012 tarihleri arasında yerel market ve bakkallardan temin edilmiştir.X-gal pozitif, MUG negatif, indol pozitif olan %10 (12/120) oranında şüpheli koloni tespit edilmiştir. Şüphelikolonilere biyokimyasal testler uygulanmış (API E 20 ve latex aglütinasyon testi) ve 7 (%5,83) örnekte E. coliO157:H7 tespit edilmiştir. Bu izolatlararfbO157 ve flicH7 primerleri kullanılarak PCR yöntemi ile doğrulama işlemiuygulanmıştır. İzolatlar %3,33 oranında O157 ve 0,83 oranında H7 antijeni pozitif olarak tespit edilmiştir. PCRanalizlerinin sonunda örneklerin %0,83'ünün E. coli O157:H7 olduğu gözlenmiştir

References

  • Abdul-Raouf, U.M., Ammar, M.S., Beuchat, L.R. 1996. Isolation of Escherichia coli O157:H7 from some Egyptian foods. International Journal of Food Microbiology, 29: 423-426.
  • Altekruse, S.F., Timbo, B.B., Mowbray, J.C., Bean, N.H., outbreaks of human illness in the United States, 1972 to 1992: Sanitary manufacturing practices protect consumers. Journal of Food Protection, 61:1405-1407. 1998. Cheese-associated
  • Andreoli, S.P., Trachtman, H., Acheson, D.W.K., Siegler, R.L., Obrig, T.G. 2002. Hemolytic uremic syndrome: epidemiology, pathophysiology, and therapy. Pediatric Nephrology, 17: 293-297.
  • Beutin, L., Krause, G., Zimmermann, S., Kaulfuss, S., Gleier, K. 2004. Characterization of Shiga toxin- producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. Journal ofClinical Microbiology, 42: 1099-1108.
  • Blanco, J., Blanco, M., Blanco, J.E., Mora, A., González, E..A., Bernárdez, M.I., Alonso, M.P., Coira, A., Rodríguez, A., Rey, J., Alonso, J.M., Usera, M.A. 2003. Verotoxin-Producing Escherichia coli in Spain: Prevalence, Serotypes, and Virulence Genes of O157:H7 and non-O157 VTEC in Ruminants, Raw Beef Products, and Humans. Experimental Biology and Medicine, 228: 345-351.
  • Centers for Disease Control and Prevention (CDC). 2007 Escherichia coli O157:H7 infection associated with drinking raw milk- Washington and Oregon, November December 2005. Morbidity Mortality Weekly Report, 56: 165-167. Weagant, Feng, P., S.D., Jinneman, K. 2011. Diarrheagenic Escherichia coli. FDA-BAM.
  • Ferens, W.A., Hovde, C.J. 2011. Escherichia coli O157:H7: Animal Reservoir and Sources of Human Infection. Foodborne Pathogens and Disease, 8: 465-487.
  • Gannon, V.P.J., D’souza, S., Graham, T., King, R.K., Rahn, K., Read, S. 1997. Use of flagellar H7 gene as a target in multiplex PCR assay and improved specificity and identification of enterohaemorrhagic Escherichia coli Microbiology, 35: 656-662. Journal of Clinical
  • Govaris, A., Koidis, P., Papatheodorou, K. 2002. Behavior of Escherichia coli O157:H7 in sour milk, cow’s milk yogurt and ewe’s milk yogurt. Journal of Dairy Research, 69: 655-660.
  • Honish, L., Predy, G., Hislop, N., Chui, L., Kowalewska-Grochowska, K., Trottier, L., Kreplin, C., Zazulak, I. 2005. An outbreak of Escherichia coli O157:H7 hemorrhagic colitis associated with unpasteurized Gouda cheese. Canadian Journal of Public Health, 96: 182-184.
  • Karmali, M.A. 1989. Infection by verotoxin-producing Escherichia coli. Clinical Microbiology Reviews, 2: 15-38.
  • Morgan, D., Newman, C.P., Hutchinson, D.N., Walker, A.M., Rowe, B., Majid, F. 1993.Verotoxin producing associated with the consumption of yoghurt. Epidemiology and Infection, 111: 181-187.
  • Öksüz, Ö., Arici, M., Kurultay, S., Gümüş, T. 2004. Incidence of Escherichia coli O157 in raw milk and white pickled cheese manufactured from raw milk in Turkey. Food Control, 15, 453-456.
  • Paton, A.W., Paton, J.C. 1998. Detection and Characterization of Shiga Toxigenic Escherichia coli by Using Multiplex PCR Assays for stx1, stx2, eaeA, EnterohemorrhagicE. colihlyA, rfbO111 and rfbO157. Journal of Clinical Microbiology, 36(2): 598-602.
  • Rahimi, E., Chaleshtori, S.S., Parsaei, P. 2011. Prevalence Escherichia coli O157 isolated from traditional cheese, ice creamand yoghurt in Iran. African Journal of Microbiology Research, 5(22): 3706- 3710. resistance of
  • Schlesser, J.E., Gerdes, R., Ravishankar, S., Madsen, K., Mowbray, J., Teo, A.Y.L. 2006. Survival of a five-strain cocktail of Escherichia coli O157:H7 during a 60 day aging period of cheddar cheese made from unpasteurized milk. Journal of Food Protection, 69: 90-99.
  • Schrade, J.P., Yager, J. 2001. Implication of milk and milk products in food disease in France and in different industrialized countries. International Journal of Food Microbiology, 67: 1-17.
  • Stampi, S., Caprioli, A., De Luca, G., Quaglio, P., Sacchetti, R., Zanetti, F. Escherichia coli O157 in bovine meat products in Northern Microbiology, 90: 257-262. 2004. Detection of
  • Italy. International Journal of Food
  • Tobias, J., Vutukuru, S.R. 2012. Simple and rapid multiplex PCR for identification of the main human diarrheagenicEscherichia Research. 167 (9):564-570. coli. Microbiological
There are 20 citations in total.

Details

Primary Language English
Journal Section MİKROBİYOLOJİ (Microbiology)
Authors

Özlem Turgay

Sowayla Mıkaeel This is me

Publication Date November 2, 2015
Published in Issue Year 2015 Volume: 18 Issue: 1

Cite

APA Turgay, Ö., & Mıkaeel, S. (2015). Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese. KSÜ Doğa Bilimleri Dergisi, 18(1), 31-34. https://doi.org/10.18016/ksujns.94848
AMA Turgay Ö, Mıkaeel S. Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese. KSÜ Doğa Bilimleri Dergisi. November 2015;18(1):31-34. doi:10.18016/ksujns.94848
Chicago Turgay, Özlem, and Sowayla Mıkaeel. “Using Culture and PCR Technique for Detection of Escherichia Coli O157:H7 in Duhok Cheese”. KSÜ Doğa Bilimleri Dergisi 18, no. 1 (November 2015): 31-34. https://doi.org/10.18016/ksujns.94848.
EndNote Turgay Ö, Mıkaeel S (November 1, 2015) Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese. KSÜ Doğa Bilimleri Dergisi 18 1 31–34.
IEEE Ö. Turgay and S. Mıkaeel, “Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese”, KSÜ Doğa Bilimleri Dergisi, vol. 18, no. 1, pp. 31–34, 2015, doi: 10.18016/ksujns.94848.
ISNAD Turgay, Özlem - Mıkaeel, Sowayla. “Using Culture and PCR Technique for Detection of Escherichia Coli O157:H7 in Duhok Cheese”. KSÜ Doğa Bilimleri Dergisi 18/1 (November 2015), 31-34. https://doi.org/10.18016/ksujns.94848.
JAMA Turgay Ö, Mıkaeel S. Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese. KSÜ Doğa Bilimleri Dergisi. 2015;18:31–34.
MLA Turgay, Özlem and Sowayla Mıkaeel. “Using Culture and PCR Technique for Detection of Escherichia Coli O157:H7 in Duhok Cheese”. KSÜ Doğa Bilimleri Dergisi, vol. 18, no. 1, 2015, pp. 31-34, doi:10.18016/ksujns.94848.
Vancouver Turgay Ö, Mıkaeel S. Using Culture and PCR Technique for Detection of Escherichia coli O157:H7 in Duhok Cheese. KSÜ Doğa Bilimleri Dergisi. 2015;18(1):31-4.