Direkt Etanol Enjeksiyonlu Kırmızıbiberlerdeki Aspergillus Flavus Küflerinin Büyüme Kinetiği

Year 2017, Volume: 20 Issue: 2, 120 - 127, 04.01.2017

Ahmet Levent İnanç , Ahmet Açık

Abstract

Bu çalışmada etanol miktarların ve iki farklı sıcaklığın Aspergillus flavus küf mantarı üzerine etkisi araştırılmıştır. Taze kırmızıbiber meyveleri tüm haliyle deneysel olarak, küf gelişimini sağlamak için Aspergillus flavus ile kontamine edilmiştir. Daha sonra biberlere 1 ml, 3 ml, 5 ml oranlarında etanol enjekte edilmiş, 28 ve 37 °C’de 21 gün depolanmıştır. Küf sayımı sonuçlarına göre etanolün küf gelişimi üzerine oldukça etkili olduğu bulunmuştur (p<0.05). 28 ve 37 °C’deki etanol ilave edilmemiş (0 ml) kontrol örneklerindeki küf miktarlarında hızlı bir büyüme gözlemlenirken, kırmızıbiberlere enjekte edilen etanolün miktarı arttıkça küf gelişiminin azaldığı tespit edilmiştir (p<0.05). 37 °C’ de ki küf miktarlarının 28 °C’ de ki küf miktarlarına göre aynı günler için daha fazla olduğu gözlemlenmiştir (p<0.05). Logaritmik evredeki Aspergillus flavus’ların büyüme hız sabitlerini (k) belirlemek için birinci mertebeden hız denklemi kullanılmıştır. Her iki sıcaklıktaki k değerleri en yüksek kontrol örneklerinde bulunmuştur (28 °C’ de 1.13 ve 37 °C’ de 1.22 kob g^-1 gün^-1). 1, 3 ve 5 ml etanol enjekte edilmiş örneklerde ise sırayla 28 °C için 0.38, 0.23 ve 0.19 kob g^-1 gün^-1; 37 °C için 0.37, 0.26 ve 0.23 kob g^-1 gün^-1olduğu belirlenmiştir.

Anahtar Kelimeler: Aspergillus flavus, büyüme hız sabiti, etanol, kırmızıbiber, kinetik

 

Growth Kinetics of Aspergillus Flavus Moulds in Red Pepper with Direct Ethanol Injection

 

ABSTRACT: In this study, the effects of two different temperatures and different ethanol ratios on Aspergillus flavus moulds were investigated. Fresh whole red pepper fruits were experimentally contaminated with Aspergillus flavus to achieve mold growth. Then, Aspergillus flavus inoculated red peppers were injected ethanol (96%) at the ratios of 1, 3 and 5 ml and stored at 28 ve 37 °C for 21 days. The study showed that ethanol was highly effective on the mold growth (p<0.05). Albeit the rapid mould growth was observed in control samples without ethanol (0 ml) at 28 and 37 ° C, the mold growths in the ethanol injected samples decreased by increasing amount of ethanol. The mould amounts in all red pepper samples at 37°C were found to be greater than those of 28 °C for the same storage times. First order kinetic equation was used to determine the growth rate constant (k) of Aspergillus flavus. The highest k values were found in control samples for both two temperatures (1.13 cfu g^-1 day^-1 at 28 °C, 1.22 cfu g^-1 day^-1 at 37 °C). Additionally, the study concluded the growth ratio of  0.38, 0.23 and 0.19 cfu g^-1 day^-1 at 28 °C, and 0.37, 0.26 and 0.23 cfu g^-1 day^-1 at 37 °C for 1, 3 and 5 ml ethanol injected samples, respectively.

Key words: Aspergillus flavus, ethanol, growth rate constant, kinetics, red pepper 

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