Evaluation of Regulatory T-Cells (Tregs) in Benign Prostatic Pathologies: A Pilot Study

Erhan Ateş; Akın Amasyalı; Erman Oryaşın; İrfan Yavaşoğlu; Mustafa Yılmaz; Bülent Bozdoğan; Haluk Erol

doi:10.17517/ksutfd.820604

EN TR

Evaluation of Regulatory T-Cells (Tregs) in Benign Prostatic Pathologies: A Pilot Study

Abstract

Objective: We aimed to evaluate the number and function of regulatory T (Treg) cells in peripheral blood and prostate tissues of patients with histopathologically diagnosed benign prostate hyperplasia (BPH) and asymptomatic chronic prostatitis. Material and Methods: Blood and histopathological data of 19 patients (BPH=10, ACP=9) that underwent transurethral prostate resection were evaluated. Treg cell count in peripheral blood and prostatic tissue with flowcytometry, Forkhead box P3 (FOXP3) expression in prostatic tissue by reverse transcription polymerase chain reaction (PCR), and IL-17 measurement in blood samples with ELISA were performed. Results: Flowcytometric analyses showed that mean CD4+T cell count and mean FOXP3 levels in both peripheral blood (CD4+T, p= 0.752; FOXP3, p= 1.000) and prostate tissue (CD4+T, p= 0.458; FOXP3, p= 0.590) were higher in the BPH group compared to the chronic prostatitis group. However this difference was not statistically significant. Similarly, the mean blood IL-17 levels were also higher in BPH groups, but the difference was not statistically significant (p= 0.870). The PCR analyses showed that mean FOXP3 gene expression in the tissue was higher in the chronic prostate group, but again there was no statistically significant difference between the groups (p= 0.116). Conclusion: Since no statistically significant difference was found between BPH and chronic prostatitis in terms of Treg cell number and function in peripheral blood and prostatic tissue, our study supports the thesis that both these pathologies could be autoimmune inflammatory diseases.

Keywords

Autoimmunity , Inflammation , Benign Prostate Hyperplasia , Chronic Prostatitis , FOXP3 , Regulatory T cells

Benign Prostatik Patolojilerde Regülatuvar T Hücrelerinin (Treg) Değerlendirilmesi: Pilot Çalışma

Öz

Amaç: Histopatolojik olarak benign prostat hiperplazisi (BPH) ve asemptomatik kronik prostatit(AKP) tanısı alan hastaların periferik kan ve prostat dokularındaki regülatuvar T (Treg) hücrelerinin sayı ve işlevini değerlendirmeyi amaçladık. Gereç ve Yöntemler: Transüretral prostat rezeksiyonu yapılan 19 hastanın (BPH = 10, AKP = 9) kan ve histopatolojik verileri değerlendirildi. Flowsitometri ile periferik kan ve prostat dokusunda Treg hücre sayımı, revers transkripsiyon polimeraz zincir reaksiyonu (PCR) ile prostatik dokuda Forkhead box P3 (FOXP3) ekspresyonu ve ELISA ile kan örneklerinde IL-17 ölçümü yapıldı. Bulgular: Flowsitometrik analizler, hem periferik kanda (CD4 + T, p = 0.752; FOXP3, p = 1.000) hem de prostat dokusunda (CD4 + T, p = 0.458; FOXP3, p = 0.590) ortalama CD4 + T hücre sayısı ve ortalama FOXP3 düzeylerinin BPH grubunda kronik prostatit grubuna göre daha yüksek olduğunu göstermiştir. Ancak bu fark istatistiksel olarak anlamlı değildi. Benzer şekilde, ortalama IL-17 seviyeleri de BPH grubunda yüksekti, ancak fark istatistiksel olarak anlamlı değildi (p = 0.870). PCR analizleri, dokudaki ortalama FOXP3 gen ekspresyonunun kronik prostat grubunda daha yüksek olduğunu, ancak yine gruplar arasında istatistiksel olarak anlamlı bir fark olmadığını gösterdi (p = 0.116). Sonuç: Periferik kandaki ve prostatik dokusundaki Treg hücre sayısı ve fonksiyonu açısından BPH ile kronik prostatit arasında istatistiksel olarak anlamlı bir fark bulunamadığından, çalışmamız her iki patolojinin de otoimmün inflamatuvar hastalıklar olabileceği tezini desteklemektedir.

Anahtar Kelimeler

Otoimmünite , Enflamasyon , Benign Prostat Hiperplazisi , Kronik Prostatit , FOXP3 , Regülatuvar T hücreleri

References

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Details

Primary Language

English

Subjects

Health Care Administration

Journal Section

Research Article

Authors

Erhan Ateş ^*
0000-0002-9677-5673
Türkiye

Akın Amasyalı This is me
0000-0003-3921-464X
United States

Erman Oryaşın
0000-0003-1242-7434
Türkiye

İrfan Yavaşoğlu This is me
0000-0003-1703-2175
Türkiye

Mustafa Yılmaz This is me
0000-0002-3353-6595
Türkiye

Bülent Bozdoğan
0000-0003-2469-9728
Türkiye

Haluk Erol
0000-0003-4101-7565
Türkiye

Publication Date

February 8, 2021

Submission Date

November 3, 2020

Acceptance Date

November 27, 2020

Published in Issue

Year 1970 Volume: 16 Number: 1

DOI

https://doi.org/10.17517/ksutfd.820604

IZ

https://izlik.org/JA77ET64HT

AMA

1.Ateş E, Amasyalı A, Oryaşın E, et al. Evaluation of Regulatory T-Cells (Tregs) in Benign Prostatic Pathologies: A Pilot Study. KSU Medical Journal. 2021;16(1):1-6. doi:10.17517/ksutfd.820604

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