The effects of hydrogen sulfide, carbon monoxide and nitric oxide on pentylenetetrazole-induced seizures in rats

Year 2020, Volume: 15 Issue: 3, 1 - 6, 16.10.2020

Ezgi Eroğlu , Çiğdem Çengelli Ünel Nuşin Harmancı , Zuhal Kaltuş Cansu Kılıç Hatice Doğan , Rabiye Altınbaş , Kevser Erol

https://doi.org/10.17517/ksutfd.681282

Abstract

Objective: It is aimed to investigate the anti-convulsant effects of bioactive gas mediators hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) in pentylenetetrazole-induced convulsions in rats.
Material Methods: Eighty male Wistar-Albino rats randomly divided into ten groups. Convulsions was induced by administering 60 mg/kg pentylentetrazole intraperitonally. Diazepam, NaHS, CORM-2, L-arginine and L-NAME were administred 60 minutes after pentylentetrazole. Seizure latency, seizure duration and seizure score were evaluated. Data were analyzed by the Kruskal Wallis test followed by posthoc analyses with Tukey. P <0.05 was considered statistically significant.
Results: Diazepam (2 mg/kg) extended the seizure latency and no significant difference was found in other groups. Diazepam (1 and 2 mg/kg) and NaHS (10 mg/kg) reduced seizure duration. NaHS (5 mg/kg), CORM-2 (5 and 10 mg/kg), L-arginine (30 and 100 mg/kg) and L-NAME (30 mg/kg) increased the seizure score significantly as compared to diazepam (1 and 2 mg/kg) groups.
Conclusion: NaHS (10 mg/kg) may be protective in pentylentetrazole-induced seizures.

Keywords

epilepsy , hydrogen sulfide , carbon monoxide , nitric oxide , seizures

Project Number

2019/11D05

Hidrojen sülfür, karbon monoksit ve nitrik oksidin sıçanlarda pentilentetrazol indüklü nöbetler üzerindeki etkileri

Abstract

Amaç: Sıçanlarda pentilentetrazol indüklü konvülsiyonlarda biyoaktif gaz mediyatörler hidrojen sülfür (H2S), karbon monoksid (CO) ve nitrik oksid (NO)'in anti-konvülsan etkilerinin araştırılması amaçlanmıştır.
Gereç Yöntem: Seksen erkek Wistar-Albino sıçan rastgele on gruba ayrılmıştır. Konvülsiyonlar, intraperitonal olarak verilen 60 mg/kg pentilentetrazol ile indüklendi. Pentilentetrazolden 60 dakika sonra Diazepam, NaHS, CORM-2, L-arginin ve L-NAME uygulandı. Nöbet latensi, nöbet süresi ve nöbet skoru değerlendirildi. Veriler Kruskal Wallis testi ve ardından Tukey ile analiz edildi. P <0.05 istatistiksel olarak anlamlı kabul edildi.
Bulgular: Diazepam (2 mg/kg) nöbet latensini uzattı, diğer gruplarda ise anlamlı bir fark bulunmadı. Diazepam (1 ve 2 mg/kg) ve NaHS (10 mg/kg) nöbet süresini azalttı. NaHS (5 mg/kg), CORM-2 (5 ve 10 mg/kg), L-Arjinin (30 ve 100 mg / kg) ve L-NAME (30 mg / kg), nöbet skorunu diazepam ile karşılaştırıldığında önemli ölçüde artırdı.
Sonuç: NaHS (10 mg/kg) pentilentetrazole bağlı nöbetlerde koruyucu olabilir.

Keywords

epilepsi , hidrojen sülfür , karbon monoksid , nitrik oksid , nöbetler

Supporting Institution

Eskişehir Osmangazi Üniversitesi Tıp Fakültesi Bilimsel Araştırma Projeleri Komisyonu

Project Number

2019/11D05

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