Malign Mezotelyoma ile Reaktif Mezotelyal Hiperplazi ayrımında, effüzyon materyallerinde GLUT-1, CD147 ve ProExC’nin tanısal değeri

Year 2021, Volume: 16 Issue: 2, 165 - 171, 07.07.2021

Tolga Bağlan Nural Onaral Erdoğan

https://doi.org/10.17517/ksutfd.866929

Abstract

Amaç: Effüzyon materyallerinde malign mezotelyoma ile reaktif mezotelyal hiperplazisi ayırıcı tanısı önemli bir sorundur. Bu sorunun çözümü için immünhistokimyasal antikorlar yaygın olarak kullanılmaktadır. Bu çalışmanın amacı, MM'yi RMH'den ayırt etmede GLUT-1, CD147 ve ProExC’nin tanısal değerini belirlemektir.
Gereç ve yöntemler: İmmünhistokimyasal çalışma, klinik ve histopatolojik korelasyonu olan 26 MM olgusu ile 26 RMH olgusuna ait hücre bloğu kesitlerinden yapıldı. Antikorlar; boyanma yüzdesi ve boyanma şiddetine göre semikantitatif olarak değerlendirildi. Herbir antikorun tek başına ve kombine tanısal performansları hesaplandı.
Bulgular: GLUT-1 ile 26 MM olgusunun 25’inde (%96.1), 26 RMH olgusunun ise 6’sında (%23) immünhistokimyasal ekspresyon izlendi. MM ve RMH olguları arasında GLUT-1 ile boyama yüzdesi ve yoğunluğu açısından istatistiksel olarak anlamlı fark vardı (p <0,001). MM ve RMH olgularının tümünde CD147 ile pozitiflik saptandı. CD147 ile MM ile RMH olguları arasında istatistiksel olarak anlamlı bir fark yoktu. ProExC’nin ortalama boyanma yüzdesi, MM olgularında %14, RMH olgularında ise %3,5 bulundu (p <0,001). MM’yi RMH’den ayırt etmede ProExC’nin en iyi kesim değeri %7 olarak hesaplandı.
Sonuç olarak GLUT-1, MM'nin RMH'den ayırıcı tanısını sağlayan duyarlı ve özgül bir immünohistokimyasal belirteçtir. ProExC, MM'yi RMH'den ayırmada faydalı olabilir. CD147 ise MM ile RMH'nin ayırıcı tanısında yararlı değildir.

Keywords

GLUT-1, CD147, ProExC, Mezotelyoma, Effüzyon, İmmünhistokimya

Supporting Institution

Ankara Üniversitesi Araştırma Fonu

Project Number

13L3330007

Diagnostic value of GLUT-1, CD147, ProExC in the differantial diagnosis between malign mesothelioma and reactive mesothelial hyperplasia in effusion materials

Abstract

Objective: The differential diagnosis of malignant mesothelioma (MM) and reactive mesothelial hyperplasia (RMH) is an important problem in effusion materials. Immunohistochemical antibodies are widely used to solve this problem. The purpose of this study is to determine the diagnostic value of GLUT-1, CD147 and ProExC in distinguishing MM from RMH.
Material and methods: Immunohistochemical studies were performed on cell block sections from 26 cases of clinic and histopathologic confirmed MM and 26 RMH. The antibodies were evaluated semi-quantitatively based on the percentage and intensity of staining. The diagnostic performance of each antibody alone and combined was calculated.
Results: Immunohistochemical GLUT-1 expression was seen in 25 of 26 (%96.1) MM and in 6 of 26 (%23) RMH. There was statistically significant difference between MM and RMH cases in terms of the percentage and intensity of staining with GLUT-1 (p <0.001). CD 147 was found positive in %100 of MM cases and RMH cases. There was not statistically significant difference between MM and RMH cases with CD147. The mean staining percentage of ProExC was found %14 in MM cases and %3.5 in RMH cases (p <0.001). The best cut-off value of ProExC in distinguishing MM from RMH was calculated as 7%.
In conclusion GLUT-1 is a sensitive and specific immunohistochemical marker enabling differential diagnosis of MM from RMH. ProExC can be useful in differentiating MM from RMH. CD147 is not useful in the differential diagnosis of MM and RMH.

Keywords

GLUT-1, CD147, ProExC, Mesothelioma, Effusion, Immunohistochemistry

Project Number

13L3330007

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