The Effect of Different Doses of Amantadine on Lung Tissue In Hepatıc Ischemia Reperfusion Injury in Rats

Abstract

Objective: N-Methyl D-Aspartate (NMDA) receptor blockers have been shown to have protective effects against ischemia/reperfusion (I/R) injury in various tissues. The aim of this study was to investigate the effects of 90 ve 135 mg/kg doses of amantadine on lung in hepatic I/R injury. Method: The rats were randomly divided into six groups: Group Sham, Group I/R, Group Amantadine-90, Group Amantadine-135, Group I/R-90 and Group I/R-135. In I/R, an atraumatic vascular clamp was applied to the structures in the left portal triad for 45 minutes and reperfusion period was 2 hours after ischemia. Malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) enzyme levels were performed the lung tissue and tissues were examined histopathologically. Result: A significant difference was found between the groups in terms of MDA, SOD, CAT levels (respectively; p < 0.001, p=0.008, p < 0.001). A significant difference was found between the groups in terms of lung tissue neutrophil/lymphocyte infiltration scores and alveolar wall thickening scores (respectively p=0.009, p=0.002). Conclusion: The biochemical and histopathological results of the present study suggested that amantadine, like other NMDA antagonist agents, may have a protective effect on lung tissues against the damage caused by hepatic I/R injury. Although we observed significant improvements after the administration of both doses studied, there was no significant difference between these two doses in terms of their success in protecting against distant organ lung injury. Amantadine appears promising as a therapeutic agent in treatment.

Keywords

• Amantadine
• Ischemia reperfusion injury
• Lung injury
• Remote organ damage

Sıçanlarda Hepatik İskemi Reperfüzyon Hasarında Farklı Dozlarda Amantadin'in Akciğer Dokusu Üzerine Etkisi

Abstract

Amaç: N-Metil D-Aspartat (NMDA) reseptör blokerlerinin çeşitli dokularda iskemi/reperfüzyon (I/R) hasarına karşı koruyucu etkileri olduğu gösterilmiştir. Bu çalışmanın amacı, hepatik I/R hasarında 90 ve 135 mg/kg dozlarındaki amantadinin akciğer üzerindeki etkilerini araştırmaktır. Gereç ve Yöntem: Sıçanlar her rastgele altı gruba ayrıldı: Grup Sham, Grup I/R, Grup Amantadin-90, Grup Amantadin-135, Grup I/R-90 ve Grup I/R-135. I/R'de sol portal triaddaki yapılara 45 dakika süre ile atravmatik vasküler klemp uygulandı ve iskemi sonrası 2 saat reperfüzyon uygulandı. Akciğer dokusunda malondialdehit (MDA), süperoksit dismutaz (SOD) ve katalaz (CAT) enzim düzeyleri ölçüldü ve dokular histopatolojik olarak incelendi. Bulgular: MDA, SOD, CAT düzeyleri açısından gruplar arasında anlamlı fark bulundu (sırasıyla; p < 0,001, p=0,008, p < 0,001). Akciğer dokusu nötrofil/lenfosit infiltrasyon skorları ve alveolar duvar kalınlaşma skorları açısından gruplar arasında anlamlı fark bulundu (sırasıyla p=0,009, p=0,002). Sonuç: Bu çalışmanın biyokimyasal ve histopatolojik sonuçları, hem 90 hem de 135 mg/kg dozdaki amantadinin, diğer NMDA antagonist ajanları gibi, karaciğer I/R hasarının neden olduğu hasara karşı akciğer dokuları üzerinde koruyucu bir etkiye sahip olabileceğini göstermiştir. Çalışılan her iki dozun uygulanmasından sonra önemli gelişmeler gözlemlememize rağmen, uzak organ akciğer hasarına karşı korumadaki başarıları açısından bu iki doz arasında anlamlı bir fark yoktu. Amantadin, tedavide terapötik bir ajan olarak umut verici görünmektedir.

Keywords

• Amantadin
• İskemi Reperfüzyon Hasarı
• Akciğer Hasarı
• Uzak Organ Hasarı

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