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Abstract

Objective: The aim of this study is to evaluate the presence of Ki 67 proliferation zone of active areas and p53 mutation in colorectal polyps and to compare the results between the polyp types. Methods: Our study included 142 colon polyps. Studies based on the presence of dysplastic areas and dysmorphism in the polyp types that were considered to be non–neoplastic showed that a different pathway may have a role in malignant transformation. The cases were reclassified according to WHO 2010 classification. The tissue sections were stained by immunohistochemistry for Ki 67 and p53 Results: In our study Ki 67 staining was seen 74.5% in the lower zone of the crypt with HP. Ki 67 staining was seen in both the lower zone (40.9%) and the upper zone (56.1%) of the crypt with AP. In almost all cases, low rate of the p53 staining was observed in the ½ lower zone of crypts. The rate of p53 staining in the upper ½ zone of the crypts was 50% in AP cases. The staining ratio was low in HP and SSA/P Conclusion: The evaluation of polyp histopathology and Ki 67, p53, and molecular genetic with serologic characteristics of patients are required in parallel reviews

Keywords

• Colon Polyps, Ki 67, p53

Kolon Poliplerinde P53 Tümör Supresör Gen ve Ki 67 Proliferatif İndeksin Değerlendirilmesi

Abstract

Kolon Poliplerinde P53 Tümör Supresör Gen ve Ki 67 Proliferatif İndeksin Değerlendirilmesi

Keywords

• Kolon Polipleri, Ki 67, p53

References

1. Bosman FT, Carneiro F, Hruban H. WHO Classification of Tumours of the Digestive System, 4th ed., Lyon: IARC Press, 2010:132-66.
2. Kumar V, Abbas A, Fausto N. Hastalığın Patolojik Temeli. 7. baskı., Güneş Tıp Kitabevleri, 2009:90-303.
3. Rosai J. Special techniques in surgical pathology. In: Rosai and Ackerman’s Surgical Pathology. Tenth ed., New York: Mosby, 2011:65,858.
4. Sheikh RA, Min BH, Yasmeen S, et al. Correlation of Ki 67, p53, and Adnab-9 immunohistochemical Staining and Ploidy with Clinical and Histopathologic Features of Severely Displastic Colorectal Adenomas. Dig Dis Sci 2003;48(1):223-9.
5. Urruticoechea A, Smith IE, Dowsett M. Proliferation Marker Ki 67 in Early Breast Cancer. J Clin Oncol. 2005;23(28):7212-20.
6. Higuchi T, Sugihara K, Jass JR. Demographic and pathological characteristics of serrated polyps of colorectum. Histopathology 2005; 47(1): 32-40.
7. Ngo NT, Tan E, Tekkis P, et al. Differantial expression of p53 and p504s in hyperplastic polyp, sessile serrated adenoma and traditional serrated adenoma. Int J Colorectal Dis 2010;25(10):1193-200.
8. Baselga J, Norton L. Focus on breast cancer. Cancer Cell 2002;1(4):319-22.

9. Werdner N, Cote RJ, Suster S. Modern Surgical Pathology, volume 1, 1st ed. Philadelphia: W.B. Saunders Company, 2003:757-63.
10. Farris AB, Misdraji J, Srivastava A, et al. Sessile serrated adenoma: challenging discrimination from other serrated colonic polyps. Am J Surg Pathol 2008; 32(1):30-5.
11. O’Brien MJ, Yang S, Clebanoff JL, et al. Hyperplastic (serrated) polyps of the colorectum: relationship of CpG island methylator phenotype and K-ras mutation to location and histologic subtype. Am J Surg Pathol 2004; 28(4):423-34.
12. Kambara T, Simms LA, Whitehall VL, et al. BRAF mutation is associated with DNA methylation in serrated polyps and cancers of the colorectum. Gut 2004; 53(8):1137-44.
13. Li SC, Burgart L. Histopathology of serrated adenoma, its variants, and differentiation from conventional adenomatous and hyperplastic polyps. Arch Pathol Lab Med 2007;131(3):440-5.
14. Jaramillo E, Tamura S, Mitomi H. Endoscopic appearance of serrated adenomas in the colon. Endoscopy 2005; 37(3):254-60 .
15. Sinicrope FA, Ruan SB, Cleary KR, et al. Tumorigenesis bcl-2 and p53 Oncoprotein Expression during Colorectal. Cancer Res 1995; 55(2):237-41.
16. Ishi T, Notohara K, Umapathy A, et al. Tubular adenomas with minor villous changes show molecular features characteristic of tubulovillous adenomas. Am J Surg Pathol 2011; 35(2):212-20.
17. Yantiss RK, Oh KY, Chen YT, et al. Filiform serrated adenomas: a clinicopathologic and immunophenotypic study of 18 cases. Am J Surg Pathol 2007; 31(8):1238-45.
18. Shiota G, Ishida M, Noguchi N. Circulating p53antibody in patients with colorectal cancer: relation to clinicopathologic features and survival.Dig Dis Sci 2000; 45(1):122-8.
19. Fujita K, Yamamoto H, Matsumoto T. Sessile serrated adenoma with early neoplastic progression: a clinicopathologic and molecular study. Am J Surg Pathol 2011; 35(2):295-304.
20. Snover DC. Serrated polyps of the large intestine. Semin Diag pathol 2005; 22(4):301-8.
21. Tarlakovic EE, Gomez JD, Driman DK, et al. Sessile serrated adenoma vs traditional serrated adenoma. Am J Surg Pathol 2008; 32(1):21-9.