Kistik Nodüler Guatrlı Olguların Tiroid Hücrelerindeki AgNOR Sayısı ve AgNOR Yüzey Alanı/Çekirdek Alanı Oranının Yaş ve Cinsiyete göre Karşılaştırılması

Year 2012, Volume: 4 Issue: 2, 31 - 35, 01.08.2012

Eröz R

Abstract

Amaç: Gümüş seven çekirdekçik organize edici bölgeler ile ilişkili proteinler (AgNORs) akrosentrik kromozomların ikincil boğumlarındaki rDNA genleri tarafından sentezlenirler vetiroid rahatsızlıkları dahil çeşitli hastalıklarda çeşitli rollere sahiptirler. Bizim amacımız kistik nodüler guatrlı bireylerin tiroid hücrelerindeki AgNOR sayısı ve AgNOR yüzey alanı/çekirdek alanı oranının yaş ve cinsiyete göre karşılaştırılmasıdır. Gereç Yöntem: Bu çalışmaya ince iğne aspirasyon biyopsisi (İİAB) kistik nodüler guatr ile uyumlu olan 18 bayan (yaş aralığı 26–78) ve 17 erkek birey dahil edildi. Bu biyopsi materyalleri AgNOR ların tespiti için spesifik bir protokole göre boyandı. Her bir birey için 100 çekirdek değerlendirildi ve her bir hücrenin AgNOR sayısı ve AgNOR yüzey alanı/çekirdek alanı (NORa/TÇa) oranı her bir grup için tespit edildi. Bulgular: Kistik nodüler guatr’lı bayan hastaların AgNOR sayısı (%2,05±0,46) ile erkek hastaların AgNOR sayısı (%2,35±0,66) arasında istatistiksel olarak anlamlı bir fark yoktu (p=0.131). Bayan hastaların NORa/TÇa oranı (%6,53±1,21) ile erkek hastaların NORa/TÇa oranı (%7,03±1,12) arasında da istatistiksel olarak anlamlı bir fark saptanmadı (p=0.211). Buna ilaveten erkek hastalarda ve bayan hastalarda hem AgNOR sayısı ile yaş (r=0.085 p=0.627)hem de NORa/TÇa oranı ile yaş arasında anlamlı bir korelasyon yoktu (r=0.286; p=0.096). Sonuç: Kistik nodüler guatrlı hastalarda hem cinsiyet hem de yaş ile AgNOR protein sentezi arasında bir ilişki yoktur

Keywords

NOR, AgNORs, kistik nodüler guatr, tiroid, rDNA

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