Arjirofilik Nükleolar Organize Edici Bölgeler Non ST Elevasyonlu Miyokard İnfarktüsünde Hipoksik Yanıtın Yeni bir Belirteci Olabilir mi?

Year 2022, Volume: 14 Issue: 1, 132 - 141, 14.03.2022

İbrahim Halil Damar Recep Eröz

https://doi.org/10.18521/ktd.1073568

Cited By: 1

Abstract

Amaç: Non-ST elevasyonlu miyokard enfarktüsü (NSTEMI), bir akut koroner sendrom türüdür ve görülme sıklığı ST elevasyonlu miyokard enfarktüsüne benzer şekilde yüksektir. Nükleolar organize edici bölgeler (NORs), akrosentrik kromozomun ikincil yapısında yer alır ve bazıları arjirofilik olan proteinlerden ve ribozomal DNA'dan (rDNA) oluşur. NSTEMI hastalarında, hipokside artan AgNOR proteinlerinin değişimini araştırmayı amaçladık.
Gereç ve Yöntem: Toplam 125 katılımcı, 63 NSTEMI hastası ve herhangi bir akut koroner sendrom tanısı olmayan 62 gönüllü çalışmaya dahil edildi. Bütün hastalara ekokardiyografi yapıldı ve her birey için hem ortalama AgNOR sayısı hem de toplam AgNOR alanı/toplam nükleer alan (TAA/TNA) tespiti yapıldı.
Bulgular: Ortalama AgNOR sayısı ve TAA/TNA oranı NSTEMI grubunda kontrole göre anlamlı derecede yüksekti (p<0.001). TAA/TNA ile, kreatinin, hemoglobin, WBC(μl/ml), monosit, nötrofil, nötrofil/lenfosit oranı, monosit/lenfosit oranı arasında istatistiksel olarak anlamlı ilişkiler tespit edildi (tümü için p<0.05). Ayrıca ortalama AgNOR sayısı ile, açlık kan şekeri, HDL, WBC(μl/ml), monosit, nötrofil ve EF arasında istatistiksel olarak anlamlı ilişkiler tespit edildi (p<0.001).
Sonuç: Her iki AgNOR protein miktarı, NSTEMI için bir markır olarak kullanılabilir. NSTEMI'de bazı prognostik bilgiler sağlayarak sonuçların tahmin edilmesine de katkıda bulunabilir.

Keywords

AGNOR, Hipoksi, NOR, NSTEMI

May Argyrophilic Nucleolar Organizer Regions be the New Marker of a Hypoxic Response in Non ST Elevation Myocardial İnfarction?

Abstract

Objective: Non-ST elevation myocardial infarction (NSTEMI) is a type of acute coronary syndrome and its’ incidence is similarly high to ST-elevation myocardial infarction. Nucleolar organizing regions (NORs) are located of the secondary structure of acrocentric chromosome and composed of proteins and ribosomal DNA (rDNA) some of which are argyrophilic. We aimed to investigate the change of AgNOR proteins, which increase in hypoxia, in patients with NSTEMI.
Methods: A total of 125 participants, 63 patients with NSTEMI and 62 volunteers without any acute coronary syndrome were included in the study. Echocardiography was performed and both mean AgNOR Number and total AgNOR area/total nuclear area (TAA/TNA) were detected for each individuals.
Results: The mean AgNOR number and TAA/TNA ratio were significantly higher in the NSTEMI group than control (p<0.001). Also, statistically significant relations between TAA/TNA and all of creatinine, hemoglobin, WBC(μl/ml), monocyte, neutrophil, neutrophil / lymphocyte ratio, monocyte / lymphocyte ratio were detected (p<0.05 for all). Also, statistically significant relations between mean AgNOR number and all of fasting blood sugar, HDL, WBC(μl/ml), monocyte, neutrophil, EF were detected (p<0.001).
Conclusions: Both AgNOR protein amounts may be used as a marker for NSTEMI. It may also contribute to the prediction of the outcomes by providing some prognostic information in NSTEMI.

Keywords

AgNOR, Hypoxia, NOR, NSTEMI.

References

• 1- Collet JP, Thiele H, Barbato E, Barthélémy O, Bauersachs J, Bhatt DL, et al. ESC Scientific Document Group. 2020 ESC Guidelines for the management of acute coronary syndromes in patients presenting without persistent ST-segment elevation. Eur Heart J. 2021 Apr 7;42(14):1289-1367. doi: 10.1093/eurheartj/ehaa575. Erratum in: Eur Heart J. 2021 May 14;42(19):1908. Erratum in: Eur Heart J. 2021 May 14;42(19):1925. Erratum in: Eur Heart J. 2021 May 13;: PMID: 32860058.
• 2- Awan MS, Daud MY, Khan M, Jehangiri AU, Adnan, Jalil S. Usefulness Of Neutrophils To Lymphocytes Ratio For Predicting Troponin-I Elevation In Patients Presenting With Suspected Nste-Acute Coronary Syndrome. J Ayub Med Coll Abbottabad. 2019 Oct-Dec;31(Suppl 1)(4):S674-S677. PMID: 31965774.
• 3- Chen H, Li M, Liu L, Dang X, Zhu D, Tian G. Monocyte/lymphocyte ratio is related to the severity of coronary artery disease and clinical outcome in patients with non-ST-elevation myocardial infarction. Medicine (Baltimore). 2019 Jun;98(26):e16267. doi: 10.1097/MD.0000000000016267. PMID: 31261596; PMCID: PMC6616945.
• 4- Damar İH, Eroz R. The Role of Macrophages in Atherosclerosis: An Overview. Erciyes Med J 2021; 43(1): 9–12.
• 5- Saritas A, Gunes H, Colakoglu S, Eroz R, Akoz A, Oktay M, et al. Are there any effects of chronic carbon monoxide exposure on argyrophilic nucleolar-organizing region-associated protein synthesis in rat myocardium? Hum Exp Toxicol. 2016 Sep;35(9):921-8. doi: 10.1177/0960327115608929. Epub 2015 Oct 13. PMID: 26462711.
• 6- Kandiş H, Afacan MA, Eröz R, Colakoglu S, Bayramoglu A, Oktay M, et al. Can argyrophilic nucleolar organizing region-associated protein amount be used for the detection of cardiac damage? Hum Exp Toxicol. 2016 Mar;35(3):323-31. doi: 10.1177/0960327115579432. Epub 2015 Mar 26. PMID: 25813961.
• 7- Gunes H, Saritas A, Eroz R, Colakoglu S. Use of argyrophilic nucleolar–organizer regionassociated protein synthesis in skeletal muscle cells for prediction of chronic carbon monoxide exposure, Toxin Reviews, 2020;39(4):349-354
• 8- Çolakoğlu S, Saritas A, Eroz R, Oktay M, Yaykasli KO, Akoz A, et al. Is one-time carbon monoxide intoxication harmless? Evaluation by argyrophilic nucleolar-organizing regions staining method. Hum Exp Toxicol. 2015 Jan;34(1):24-31. doi: 10.1177/0960327114531994. Epub 2014 May 8. PMID: 24812150.
• 9- Selvi B, Demirtas H, Eroz R, Imamoglu N. Reduction of the argyrophilic nucleolar organizing region associated protein synthesis with age in buccal epithelial cells of healthy individuals. Aging Clin Exp Res. 2015 Apr;27(2):201-8. doi: 10.1007/s40520-014-0263-6. Epub 2014 Aug 1. PMID: 25082567.
• 10- Damar İH, Eroz R. The Association of Hereditary Prothrombotic Risk Factors with ST-Elevation Myocardial Infarction. Medeni Med J. 2020;35(4):295-303. doi: 10.5222/MMJ.2020.67366. Epub 2020 Dec 25. PMID: 33717621; PMCID: PMC7945729.
• 11. Lang RM, Badano LP, Mor-Avi V, Afilalo J, Armstrong A, Ernande L, et al. Recommendations for cardiac chamber quantification by echocardiography in adults: an update from the American Society of Echocardiography and the European Association of Cardiovascular Imaging. J Am Soc Echocardiogr. 2015 Jan;28(1):1-39.e14. doi: 10.1016/j.echo.2014.10.003. PMID: 25559473.
• 12- Benn PA, Perle M. Chromosome staining and banding techniques. In: Rooney, DE, Czepulkowski, BH (eds). Human Cytogenetics, Constitutional Analysis, practical approach, Vol 1. Oxford: Oxford University Press, 1986, pp.91–118.
• 13- Lindner LE. Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). J Histochem Cytochem. 1993 Mar;41(3):439-45. doi: 10.1177/41.3.8429207. PMID: 8429207.
• 14- Rasband WS. ImageJ, U. S. National Institutes of Health, Bethesda, Maryland, USA, https://imagej.nih.gov/ij/, 1997 –2016.
• 15-Nisari M, Eroz R, Nisari M, Ertekin T, Oktay M, Kavutcu M. Investigation of argyrophilic nucleolar organizing region. Bratisl Lek Listy. 2016;117(6):345-50. doi: 10.4149/bll_2016_068. PMID: 27546367.
• 16- Eroz R, Saritas A, Colakoglu S, Oktay M, Kandiş H. Evaluation of argyrophilic nucleolar organizing region–associated protein synthesis in femoral muscle cells of rats exposed 3000 ppm carbon monoxide gas. Konuralp Medical Journal 2016; 8: 9-13.
• 17- Caimi G, Lo Presti R, Canino B, Ferrera E, Hopps E. Behaviour of the neutrophil to lymphocyte ratio in young subjects with acute myocardial infarction. Clin Hemorheol Microcirc. 2016;62(3):239-47. doi: 10.3233/CH-151968. PMID: 26444599.14
• 18- Park JS, Seo KW, Choi BJ, Choi SY, Yoon MH, Hwang GS, et al. Importance of prognostic value of neutrophil to lymphocyte ratio in patients with ST-elevation myocardial infarction. Medicine (Baltimore). 2018 Nov;97(48):e13471. doi: 10.1097/MD.0000000000013471. PMID: 30508975; PMCID: PMC6283189.
• 19-Fan Z, Li Y, Ji H, Jian X. Prognostic utility of the combination of monocyte-to-lymphocyte ratio and neutrophil-to-lymphocyte ratio in patients with NSTEMI after primary percutaneous coronary intervention: a retrospective cohort study. BMJ Open. 2018 Oct 18;8(10):e023459. doi: 10.1136/bmjopen-2018-023459. PMID: 30341133; PMCID: PMC6196857.
• 20- Misumida, Naokia; Kobayashi, Akihiroa; Kanei, Yumikob Racial differences in the neutrophil-to-lymphocyte ratio in patients with non-ST-segment elevation myocardial infarction, Coronary Artery Disease: August 2015 - Volume 26 - Issue 5 - p 381-385 doi: 10.1097/MCA.0000000000000237
• 21-Guo TM, Cheng B, Ke L, Guan SM, Qi BL, Li WZ, et al. Prognostic Value of Neutrophil to Lymphocyte Ratio for In-hospital Mortality in Elderly Patients with Acute Myocardial Infarction. Curr Med Sci. 2018 Apr;38(2):354-359. doi: 10.1007/s11596-018-1887-0. Epub 2018 Apr 30. PMID: 30074197.
• 22- Meeuwsen JAL, Wesseling M, Hoefer IE, de Jager SCA. Prognostic Value of Circulating Inflammatory Cells in Patients with Stable and Acute Coronary Artery Disease. Front Cardiovasc Med. 2017 Jul 14;4:44. doi: 10.3389/fcvm.2017.00044. PMID: 28770211; PMCID:PMC5509763.