Ailesel Akdeniz Ateşi (AAA) Hastalarında Apoptosis-associated speck-like protein containing a CARD (ASC), TNF Like Factor 1a(TL-1a) ve B Cell Chemoattractant Chemokine Ligand 13(CXCL 13) Genlerinin Ekpresyon Düzeylerinin İncelenmesi

Year 2023, Volume: 15 Issue: 1, 59 - 68, 15.03.2023

Fatih Kurt , Recep Eröz , Kenan Kocabay

https://doi.org/10.18521/ktd.1162175

Cited By: 1

Abstract

Amaç: Bu çalışma genetik analizi Düzce Üniversitesi Araştırma ve Uygulama Hastanesi'nde yapılan ve Tell-Hashomer Kriterleri’ne göre Ailesel Akdeniz Ateşi tanısı konan hastalarda ASC (Apoptosis Associated Speck Like Protein Containing a CARD), TL-1a(TNF Like Factor 1a) ve CXCL 13(B Cell Chemoatractant Chemokine Ligand 13) genlerinin ekspresyon düzeylerini sağlıklı kontroller ile karşılaştırmak ve AAA'daki klinik önemini belirlemek amacıyla yapılmıştır.
Metod: Çalışmaya 36 hasta (20 kız, 16 erkek) ve 12 sağlıklı kontrol (7 kız, 5 erkek) dahil edildi. Her bireyin periferik kanından RNA izole edildi ve ASC, TL-1a ve CXCL 13 genlerinin ekspresyon seviyeleri belirlendi. Rutin biyokimyasal parametreler de belirlendi.
Bulgular: AAA hastalarında CXCL 13 ve TL-1a gen ekspresyon seviyeleri anlamlı olarak artarken, ASC geninin ekspresyon seviyesinin arttığı ancak anlamlı olmadığı bulundu.
Sonuç: Bu genlerin ekspresyon düzeyleri hastalığın patogenezi ile ilişkili olabilir ve bu genler hastalığın erken tanısında bir belirteç olarak kullanılabilir.

Keywords

AAA, ASC, CXCL 13, TL-1a

Supporting Institution

Düzce Üniversitesi Bilimsel Araştırma Projeleri

Project Number

2016.04.03.471

Thanks

Düzce Üniversitesi

Apoptosis-associated speck-like protein containing a CARD (ASC), TNF Like Factor 1a(TL-1a) and B Cell Chemoattractant Chemokine Ligand 13(CXCL-13) expression profiles in familial Mediterranean fever (FMF) patients

Abstract

Objectives: This study was carried out to compare the expression levels of ASC(Apoptosis Associated Speck Like Protein Containing a CARD), TL-1a(TNF Like Factor 1a) and CXCL 13(B Cell Chemoattractant Chemokine Ligand 13) genes in FMF patients According to Tell-Hashomer Criteria and Genetic analysis result in Düzce University Research and Application Hospital with healthy controls and to determine their clinical significance in FMF.
Method: 36 patients (20 girls, 16 boys) and 12 healthy controls (7 girls, 5 boys) were included in the study. RNA was isolated from the peripheral blood of each individual and expression levels of ASC, TL-1a and CXCL 13 genes were determined. Routine biochemical parameters were also determined.
Result: CXCL 13 and TL-1a gene expression levels were significantly increased in patients with FMF, the expression level of the ASC gene was found to be increased in FMF patients, but not significantly.
Conclusion: The expression levels of these genes may be related to the pathogenesis of the disease and these genes could be used as a marker in the early diagnosis of the disease.

Keywords

FMF, CXCL-13, TL-1a, ASC

Project Number

2016.04.03.471

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