Antibacterial and Anti-Biofilm Effect of Silver Nanoparticles Synthesized from Origanum majorana and Echinacea purpurea (L.) Moench Plants via Green Synthesis

Year 2024, , 48 - 52, 04.09.2024

Ayşe Karacalı Tunç , Büşra Merve Sarıtaş , Ramazan Erenler

https://doi.org/10.30934/kusbed.1389670

Abstract

Objective: Nanotechnology has gained importance in the fight against epidemics and antibiotic resistance. Nanotechnology is a potential way to prevent the increase of multidrug-resistant bacterial species. The aim of this study was to determine the antimicrobial and anti-biofilm activities of Origanum majorana and Echinacea purpurea silver nanoparticles (AgNPs) against Staphylococcus aureus (S.aureus) ATCC 25923, Escherichia coli (E.coli) 25922, Pseudomonas aureginosa (P.aureginosa) 27853, Klebsiella pneumoniae (K. pneumoniae) 700603.

Methods: Minimum Inhibitory Concentration (MIC) values of silver nanoparticles were determined by microdilution method in 96-well ELISA plates. The anti-biofilm effect of silver nanoparticles was performed by crystal violet method in 96-well microplates.

Results: The MIC value of four isolates was determined as 128 µg/ml for two nanoparticles. Only 256 µg/ml and 512 µg/ml were found for S. aureus. In vitro, anti-biofilm effect of AgNPs against biofilm forming bacteria was evaluated in a dose-dependent manner. S. aureus, AgNP synthesized from Origanum majorana plant extract (512 µg/ml) reduced biofilm formation by 92% after 24 hours of incubation. As a result of 24 hours incubation of S. aureus with AgNP (512 µg/ml) synthesized from Echinacea purpurea (L.) It was determined that biofilm formation decreased by 85%. It was observed that both different nanoparticles significantly inhibited the biofilm mass.

Conclusion: AgNPs showed antimicrobial and antibiofilm effects for standard strains. The use of AgNPs as antimicrobials is promising for the future.

Keywords

Silver Nanoparticles, antibiotic resistance, Origanum majorana, Echinacea purpurea

References

• Eissa D, Hegab RH, Abou-Shady A, Kotp YH. Green synthesis of ZnO, MgO and SiO2 nanoparticles and its effect on irrigation water, soil properties, and Origanum majorana productivity. Nat. 2022; 12(1): 5780.
• Erenler R, Dag B. Biosynthesis of silver nanoparticles using Origanum majorana L. and evaluation of their antioxidant activity. Inorg Nano-Met. 2022; 52(4): 485-492.
• Yassin MT, Mostafa AAF, Al-Askar AA, Al-Otibi FO. Facile green synthesis of silver nanoparticles using aqueous leaf extract of Origanum majorana with potential bioactivity against multidrug resistant bacterial strains. Crystals. 2022; 12(5): 603.
• Hambardzumyan S, Sahakyan N, Petrosyan M, Nasim MJ, Jacob C, Trchounian A. Origanum vulgare L. extract-mediated synthesis of silver nanoparticles, their characterization and antibacterial activities. AMB Express. 2020; 10: 1-11.
• Erenler R, Geçer EN, Bozer BM. Cytotoxic, apoptotic, and necrotic effects of silver nanoparticles biosynthesized using Origanum majorana extract. IJCT. 2022; 6(2): 142-146.
• Scandorieiro S, Rodrigues BC, Nishio EK, Panagio LA, De Oliveira AG, Durán N, Kobayashi RK. Biogenic silver nanoparticles strategically combined with Origanum vulgare derivatives: Antibacterial mechanism of action and effect on multidrug-resistant strains. Front. Microbiol. 2022; 13: 842600.
• Kolypetri S, Kostoglou D, Nikolaou A, Kourkoutas Y, Giaouris E. Chemical Composition, Antibacterial and Antibiofilm Actions of Oregano (Origanum vulgare subsp. hirtum) Essential Oil against Salmonella Typhimurium and Listeria monocytogenes. Foods. 2023; 12(15): 2893.
• Cortés ME, Bonilla JC, Sinisterra RD. Biofilm formation, control and novel strategies for eradication. Sci Against Microbial Pathog Commun Curr Res Technol Adv. 2011; 2: 896-905.
• Davey ME, O'toole GA. Microbial biofilms: from ecology to molecular genetics. MMBR. 2000; 64: 847-867.
• Flemming, Hans-Curt, and Jost Wingender. The biofilm matrix. Nat Rev Mic. 2010; 8(9): 623-633.
• Ersanli C, Tzora A, Skoufos I, Fotou K, Maloupa E, Grigoriadou K, Zeugolis DI. The Assessment of Antimicrobial and Anti-Biofilm Activity of Essential Oils against Staphylococcus aureus Strains. Ant. 2023; 12(2): 384.
• El-Ghorab AH, Behery FA, Abdelgawad MA, Alsohaimi IH, Musa A, Mostafa EM, Aboseada MA. LC/MS profiling and gold nanoparticle formulation of major metabolites from Origanum majorana as antibacterial and antioxidant potentialities. Plants. 2022; 11(14): 1871.
• Yazdanian M, Rostamzadeh P, Alam M, Abbasi K, Tahmasebi E, Tebyaniyan H, Kahnamoei MB. Evaluation of antimicrobial and cytotoxic effects of Echinacea and Arctium extracts and Zataria essential oil. AMB Express. 2022; 12(1): 1-13.
• Gecer EN, Erenler R, Temiz C, Genc N, Yildiz I. Green synthesis of silver nanoparticles from Echinacea purpurea (L.) Moench with antioxidant profile. Part. Sci. Technol. 2021; 40(1); 50-57.
• Fan Q, Zhang Y, Yang H, Wu Q, Shi C, Zhang C, et al. Effect of coenzyme Q0 on biofilm formation and attachment-invasion efficiency of Listeria monocytogenes. Food Control. 2018; 90: 274–281.
• Dinçoğlu AH. Mercanköşk (Origanum Onites L.) Bitkisinin Bazı Gıda Patojenleri Üzerine Antibakteriyel Etkinliğinin Saptanması. MAKÜ Sag. 2019; 7(2): 139-146.
• Panchal P, Malik R, Paul DR, Meena P, Tomer VK, Nehra SP. Photocatalytic activity of green synthesized AgCl nanoparticles towards E. coli bacteria. J. Nanosci. Nanotechnol. 2019; 19(8); 5249-5255.
• Semiz G, Semiz A, Mercan-Doğan N. Essential oil composition, total phenolic content, antioxidant and antibiofilm activities of four Origanum species from southeastern Turkey. Int J Food Prop. 2018; 21(1): 194-204.
• Tahmasebi E, Maskani P, Farimani MM, Mosaddad SA, Hosseini ZS, Ranjba R, Yazdanian M. Investigation of cytotoxicity and oral antibacterial effects of four biomaterials/herbal formulations. Biomass Convers Biorefin. 2023; 1-18.
• Ghajari G, Naser RH, Pecho RDC, Alhili F, Piri-Gharaghie T. Chitosan/Pectin Nanoparticles Encapsulated with Echinacea pallida: a Focus on Antibacterial and Antibiofilm Activity Against Multidrug-Resistant Staphylococcus aureus. Int J Appl Biotechnol Biochem. 2023; 1-21.
• Fathi F, Sadrnia M, Arjomandzadegan M, Mohajerani HR. In vitro and in vivo evaluation of antibacterial and anti-biofilm properties of five ethnomedicinal plants against oral bacteria by TEM. Avicenna J Phytomed. 2021; 11(2): 180-189.
• Mohanta YK, Biswas K, Jena SK, Hashem A, Abd_Allah EF, Mohanta TK. Anti-biofilm and antibacterial activities of silver nanoparticles synthesized by the reducing activity of phytoconstituents present in the Indian medicinal plants. Front. Microbiol. 2020; 11: 1143.