Türkiye’nin Muş İlinde Atlarda Anaplasma phagocytophilum’un İlk Moleküler Tespiti ve Filogenetik Analizi

Abstract

Amaç: Anaplasma phagocytophilum'un neden olduğu equine granülositik anaplasmosis (EGA), genellikle karakteristik yüksek ateş ve trombositopeni gibi kanda bir dizi değişiklik geliştiren bir hastalıktır. A. phagocytophilum' un biyolojik bulaşması intrastadial yolla sert keneler tarafından yapılmaktadır. Bu çalışmanın amacı, Türkiye'nin Muş ilinde atlarda A. phagocytophilum varlığının moleküler yöntemlerle belirlenmesidir. Yöntem: Bu çalışma için Muş ilinde bakılan 93 attan kan örnekleri alındı. Ticari ELISA kiti (IgG) ve nested PCR yapıldı. DNA’lar kandan ekstrakte edildi ve A. phagocytophilum'un 16S rRNA genini hedef alan nested PCR tekniği ile analiz edildi. Daha sonra pozitif PCR ürünleri sekanslandı. Bulgular: A. phagocytophilum'a karşı seroprevalans % 8,6 bulunurken, 6 atın (% 6,4) nested PCR sonuçları pozitif bant gösterdi. Elde edilen sekanslar, BLAST tarafından yayınlanan A. phagocytophilum amplikonlar ile %99,5 ve %100 benzerlik gösterdi. Sonuç: Yaptığımız literatür taramalarına göre bu çalışma, Türkiye'nin Muş ilinde atlarda A. phagocytophilum'un ilk moleküler verisidir. Aynı zamanda, küçük bir bölgeden izole edilen A. phagocytophilum izolatlarının, çok çeşitli konakçılardan izole edilen A. phagocytophilum ile yakından ilişkili olduğunu ortaya koymaktadır.

Keywords

• Anaplasma phagocytophilum
• Atlar
• Filogeni
• Türkiye
• PZR
• Muş

First Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Muş Province of Turkey

Abstract

Objective: Equine granulocytic anaplasmosis (EGA) caused by Anaplasma phagocytophilum is a disease that generally develop characteristic high fever and a number of changes in blood such as thrombocytopenia. The main transmission of A. phagocytophilum is hard ticks through intrastadially. The aim of this study was to determine the presence of A. phagocytophilum in horses in Muş province of Turkey by using molecular methods. Methods: In the present survey, blood samples were collected in Muş province from 93 horse. Commercial ELISA kit (IgG) and nested-PCR were performed. The DNA was extracted from blood and analysed by nested-PCR technique targeting 16S rRNA gene of A. phagocytophilum and then positive PCR products were sequenced. Results: The seroprevalence against A. phagocytophilum was 8.6% and 6 horses (6.4%) showed positive nested-PCR results. The sequences had 99.5 and 100% homology with the BLAST published A. phagocytophilum amplicons. Conclusion: To the best of our knowledge, this is the first molecular data of A. phagocytophilum in horses in Muş province of Turkey. This study also reveals that A. phagocytophilum isolates isolated from a small region are closely related to A. phagocytophilum isolated from a wide variety of hosts.

Keywords

• Anaplasma phagocytophilum
• Horses
• Phylogeny
• Turkey
• PCR
• Mus

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