Effect on Endoplasmic Reticulum Stress of the Combined Oral Contraceptives in the Liver

Year 2024, Volume: 10 Issue: 1, 1 - 7, 15.03.2024

Seval Türk Alexandra Cernomorcenco Esma Kırımlıoğlu

https://doi.org/10.30934/kusbed.1281214

Abstract

Objective: We aimed to evaluate the effects of combined oral contraceptive active ingredients ethinylestradiol, drospirenone, and ethinylestradiol+drospirenone for histopathological changes, and endoplasmic reticulum stress levels in the liver.

Methods: In the study, 37 to 8-week-old Balb/c female mice were used. Mice were randomly divided into the control, sham, ethinylestradiol, drospirenone, and ethinylestradiol+drospirenone groups. Experimental groups were administered ethinylestradiol, drospirenone, and ethinylestradiol+drospirenone with gavage for 35 days. In liver tissue sections, histopathological changes were detected with hematoxylin&eosin, orcein, Mallory's Azan, and periodic acid-Schiff, and the presence of endoplasmic reticulum stress was detected by Chop and Grp78 immunostaining.

Results: The ethinylestradiol+drospirenone group showed significant histopathological changes compared to the control group. Some degenerative changes were noted such as swelling and size differences in hepatocytes in the ethinylestradiol+drospirenone group. When compared to the control group, an increased collagen and elastic fibers density around the vena centralis was observed in the ethinylestradiol+drospirenone group. The expression level of Grp78 protein in female mice given ethinylestradiol+drospirenone was statistically significantly increased compared to the control group. The expression level of Chop protein was significantly increased in the ethinylestradiol, drospirenone, and ethinylestradiol+drospirenone groups.

Conclusion: We concluded that the use of combined oral contraceptives increases endoplasmic reticulum stress in mouse liver tissue, and as a result, it may cause liver histopathological disorders by promoting cell death.

Keywords

Combined oral contraceptive, drospirenone, ethinylestradiol, liver, Grp78, Chop

Supporting Institution

yok

Project Number

yok

Thanks

Necdet Demir was the thesis advisor of Alexandra Cernomorcenco. And his vast knowledge was consulted when performing the Combined Oral Contraceptives Method on mice. Necdet Demir died on 21.12.2022. We would like to express our gratitude to Necdet Demir for his contributions, guidance, and support in our research life.

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