Pages 10-14

Abstract

The present study was performed to evaluate the effects of lamotrigine, which is a widely used antiepileptic drug for epilepsy treatment, on oxidative stress parameters in the liver of neonates. Ten adult Wistar-albino rats and twenty newborn of these rats were used in this study. Adult rats weighing 170–250 g were divided into five groups: the pregnant control group, experimental epilepsy group, lamotigine group, lamotrigine + experimental epilepsy group and lamotrigine + epilepsy + folic acid group. An experimental penicillin acute epilepsy model was created in pregnant rats on day 13 of pregnancy. The lamotrigine group was administered lamotrigine at 25 mg/day by intraperitoneal injection starting from the first day of pregnancy. The lamotrigine + folic acid group was administered 400 microgram/kg folic acid and 25 mg/day lamotrigine by intraperitoneal injection. We then waited for the rats to give birth. Immediately after birth, the glutathione, malondialdehyde, glutathione peroxidase and superoxide dismutase levels in the newborn rat liver were measured by spectrophotometry. The lamotrigine and lamotrigine + epilepsy groups showed significantly elevated liver malondialdehide levels, while glutathione peroxidase enzyme activation was significantly decreased compared to the control group. In the group administered folic acid together with lamotrigine, the liver tissue sample for malondialdehide levels showed a significant decrease, while glutathion and glutathion peroxidase levels increased. This study on pregnant rats demonstrated that lamotrigine use can lead to hepatic oxidative damage in newborn rats, and this oxidative tissue damage was prevented by folic acid use.

Keywords

• Lamotrigine, epilepsy, pregnancy, liver, oxidative stress

Lamotrigin kullanan epileptik ratların yeni doğan yavrularının karaciğerlerinde oksidatif hasarın incelenmesi

Abstract

Bu çalışmada amacımız epilepsi tedavisinde sıkça kullanılan antiepileptik ilaçlardan lamotirijinin yalnız veya folik asit ile birlikte gebelikte kullanımının yeni doğan yavruların karaciğerlerinde oksidatif stres parametreleri üzerine etkilerini araştırmaktır. Çalışmamız 170-250 gr ağırlığında 10 adet erişkin dişi Wistaralbino türü rat ve bunlardan doğan 20 yavru rat ile yapıldı. Ratlar; Gebe ratlar kontrol grubu, deneysel epilepsi grubu, lamotrijin grubu, lamotrijin+deneysel epilepsi grubu ve lamotrijin epilepsi+folik asit olarak beş gruba ayrıldı. Gebeliğin 13. Gününde gebe ratlara deneysel penisilin akut epilepsi modeli oluşturuldu. Lamotrijin grubuna gebeliğin ilk gününden itibaren her gün intraperitoneal enjeksiyon ile 25mg/gün dozunda lamotrijin verildi. Lamotrijin+folik asit grubuna her gün intraperitoneal enjeksiyon ile 25mg/gün dozunda lamotrijin ve 400µg/kg folik asit verildi. Daha sonra gebe ratların doğum yapmaları beklendi. Doğumdan hemen sonra yeni doğan ratların karaciğerlerinde glutatyon, malondialdehit, glutatyon peroksidaz ve superoksid dismutaz seviyeleri spektrofotometrik olarak ölçüldü. Lamotrijin ve lamotrijin+epilepsi gruplarına ait biyokimyasal değerler incelendiğinde, karaciğer malondialdehit anlamlı bir şekilde yükseldiği, glutatyon peroksidaz enzim aktivasyonlarının kontrol grubuna göre istatistiksel olarak anlamlı azalmış olduğu görüldü. Folik asit ile birlikte lamotrijin verilen grupta, karaciğer doku örneklerine ait malondialdehit düzeylerinin anlamlı azaldığı, glutatyon ve glutatyon peroksidaz düzeyinin arttığı tespit edildi. Gebe ratlar üzerinde gerçekleştirmiş olduğumuz bu çalışmada, lamotrijin kullanımının yeni doğan yavruların karaciğerlerinde erken dönemde bir oksidatif hasar meydana getirebileceği ancak meydana gelen oksidatif doku hasarının folik asit kullanıldığında engellendiği biyokimyasal olarak ortaya kondu

Keywords

• Lamotrijin, epilepsi, gebelik, karaciğer, oksidatif stres

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