Pages 1-6 Evaluation of Antibacterial Activity of Root Canal Sealers Used in Primary Teeth Against E. faecalis

Abstract

Amaç: Süt dişlerinde kullanılan 7 farklı kök kanal dolgu materyalinin Enterococcus faecalis’e karşı antibakteriyel etkinliklerini in vitro ortamda değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Agar difuzyon yöntemi kullanılarak yaptığımız bu çalışmada Sure-paste, Matepex, Tg Pex, Calcicure, Endoflas FS, Sealapex, %2 lik Klorheksidin Glukonat solüsyonu ile Ca(OH)2 (Kalsiyum hidroksit) tozundan hazırlanan pat ve kontrol grubu materyali (Klorhex Oral Gel) kullanılmıştır. Enjektable formda bulunan ve karıştırılarak hazırlanan patlar Enterococcus feacalis mikroorganizmasının inokule edildiği agar petrilerinde hazırlanan çukurlara yerleştirildi. 24, 48 ve 72. saat inhibisyon süresinden sonra, oluşan inhibisyon zonları gözlendi ve ölçüldü. Her bir materyal için elde edilen 8 ölçümün ortalamaları alındı. Veriler tek yönlü varyans analizi (ANOVA) ile karşılaştırıldı.(p < 0.05) Bulgular: Çalışmada kullanılan materyallerden Sure-paste, Calcicure ve Ca(OH)2 tozu/Klorhex irrigasyon solüsyonu karışımı E.faecalis suşları üzerinde antibakteriyel etkinlik göstermiş, Metapex, Tgpex, Endoflas FS ve Sealapex ise herhangi bir aktivite göstermemiştir. Calcicure ve Ca(OH)2 tozu/Klorhex irrigasyon solüsyonu karışımı Sure-paste’den daha etkili bulunmuştur.(p < 0.05) Fakat, en geniş inhibisyon zonu Klorhex oral gel (kontrol grubu) ile bulundu. Fakat, Klorhex oral gel, Calcicure ve Ca(OH)2 tozu/Klorhex irrigasyon solüsyonu karışımı arasında anlamlı bir fark yoktur. 24-saat süresinden 48 saat süresine kadar ve 24-saat süresinden 72-saat süresine kadar geçen zamanda Sealapex’in antibakteriyel etkinliğinde değişiklik olmuştur. Sonuç: Ca(OH)2 tozu/Klorhex irrigasyon solüsyonu karışımı ve enjektabl Ca(OH)2 patlarının, Ca(OH)2, iyodoform and çinko oksit ojenol karışımlardan ve Ca(OH)2 bazlı bir kök kanal dolgu materyali olan Sealapex’den daha fazla antimikrobiyal etkinlikleri vardır.

Keywords

• Kök kanal dolgu patı, kalsiyum hidroksit, klorheksidin glukonat, süt dişi, enterococcus faecalis

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