Hipotiroidili Sıçanlarda Koenzim Q10 Uygulamasının Gdnf, Plzf, Sox3, Thy1 Genlerinin İfadesi Üzerine Etkisi

Abstract

Amaç: Hipotiroidizm, tiroid hormonlarının eksik üretilmesi sorunudur. Bu hormonlar metabolizmayı düzenler. Dolayısıyla tiroid hormon eksikliğine bağlı olarak kişinin mevcut sağlık durumu olumsuz etkilenir. Koenzim Q10 (CoQ10) güçlü antioksidan özelliklere sahip vitamin benzeri bir maddedir. Planlamış olduğumuz bu bilimsel çalışma, CoQ10'un hipotiroidizm üzerine etkilerini, önemli biyobelirteçler olan glial hücre kaynaklı nörotrofik faktör (GDNF), promyelositik lösemi çinko parmağı (PLZF), high mobility group box transcription factor 3 (SOX3) ve timosit farklılaşma antijeni 1 (THY1) ile serum düzeyinde araştırmaktır. Gereç ve Yöntemler: Dört deney hayvanı grubu oluşturuldu: Kontrol grubu (n:7); hipotiroidi grubu (n:7); CoQ10 grubu (n:7); hipotiroidi + CoQ10 grubu (n:7). Otuz birinci günde hayvanların serumları alındı ve kanda Gdnf, Plzf, Sox3, Thy1 ekspresyon düzeyleri analiz edildi. Bulgular: Dört biyobelirteçte de anlamlı sonuçlar elde edildi. Nihai sonuç olarak, hem hipotiroid patolojisi tüm bu biyobelirteçlerle ilişkilendirildi hem de CoQ10’un hipotiroidizmi olumlu yönde etkilemiştir. Sonuç: Deneysel hipotiroidizmli sıçanlarda koenzim Q10'un serum düzeyinde Plzf, Gdnf, Thy1, Sox3'ün gen ifade düzeyinde etkisi moleküler analizlerle gösterilmiştir. Koenzim Q10, tedavi sırasında serum gen ifade seviyelerini düzenlemektedir.

Keywords

• Gen ekspresyonu
• hipotiroidizm
• koenzimler
• metabolizma

THE EFFECT OF COENZYME Q10 ADMINISTRATION ON THE EXPRESSION OF Gdnf, Plzf, Sox3, Thy1 GENES IN RATS WITH HYPOTHYROIDISM

Abstract

Objective: Hypothyroidism is a problem of deficient production of thyroid hormones. These hormones regulate metabolism. Therefore, the current health status of the person is adversely affected due to thyroid hormone deficiency. Coenzyme Q10 (CoQ10) is a vitamin-like substance with strong antioxidant properties. The aim of this scientific study is to investigate the effects of CoQ10 on hypothyroidism at sera level with important biomarkers glial cell line-derived neurotrophic factor (GDNF), promyelocytic leukaemia zinc finger protein (PLZF), high mobility group box transcription factor 3 (SOX3) and thymocyte differentiation antigen 1 (THY1). Material and Methods: Four experimental animal groups were formed: Control group (n:7); hypothyroidism group (n:7); CoQ10 group (n:7); hypothyroidism + CoQ10 group (n:7). On the thirty-first day, sera of the animals were collected and Gdnf, Plzf, Sox3, Thy1 expression levels were analyzed in the blood. Results: Significant results occurred in all four biomarkers. As a final result, both hypothyroid pathology was associated with all biomarkers, and CoQ10 positively affected hypothyroidism. Conclusion: The effect of coenzyme Q10 on gene expression levels of Plzf, Gdnf, Thy1, Sox3 at sera level in rats with experimental hypothyroidism was shown by molecular analyses. Coenzyme Q10 regulates sera gene expression levels during treatment.

Keywords

• Gene expression
• hypothyroidism
• coenzymes
• metabolism

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