THE PROTECTIVE ROLE OF CARNOSINE IN LUNG INJURY FOLLOWING SINGLE LUNG VENTILATION

An Erratum to this article was published on April 27, 2026. https://dergipark.org.tr/en/pub/kutfd/article/1938076

Abstract

Objective: The aim of this study is to investigate the protective effect of carnosine on lung injury after single lung ventilation in a rat model. Material and Methods: Twenty Sprague-Dawley rats were divided into two groups for the study (n=10). We used single lung ventilation (SLV) for 60 minutes, following a 30-minute dual lung ventilation (DLV) model on all animals. In the carnosine group, animals received 250 mg/kg carnosine intraperitoneally 10 minutes before the start of the experiment. Lung samples from the control and carnosine groups were obtained for biochemical analysis and histopathological evaluation at the end of SLV and DLV. Tissue levels of superoxide dismutase (SOD), malondialdehyde (MDA), and tumor necrosis factor-alpha (TNF-α) were measured. Histopathologically, hematoxylin-eosin-stained tissues were scored for lung injury parameters such as alveolar congestion, intra-alveolar hemorrhage, presence and amount of leukocyte and lymphocyte infiltration. Results: In the carnosine group, TNF-α levels were significantly reduced compared to the control group at the end of SLV and DLV (p<0.05). However, there was no significant difference in MDA and SOD levels between the two groups after SLV and DLV (p>0.05). Histopathological evaluation showed a statistically significant reduction in polymorphonuclear leukocyte (PMNL) and lymphocyte infiltration in the carnosinegroup (p<0.05). Conclusion: We believe that carnosine has the potential for preventing injury caused by SLV in thoracic surgery procedures.

Keywords

• Single lung ventilation
• carnosine
• MDA
• SOD
• TNF-α
• alveolar congestion
• lymphocyte infiltration

Karnozinin Tek Akciğer Ventilasyonu Sonrası Oluşan Akciğer Hasarı Üzerine Koruyucu Rolü

An Erratum to this article was published on April 27, 2026. https://dergipark.org.tr/en/pub/kutfd/article/1938076

Abstract

Amaç: Bu çalışmanın amacı, sıçanlarda tek akciğer ventilasyonu sonrasında oluşan akciğer hasarı üzerine karnozininin koruyucu etkisini araştırmaktır. Gereç ve Yöntemler: Çalışma için 20 adet Sprague Dawley cinsi sıçan, eşit sayıda (n=10) iki gruba ayrılarak 60 dakika boyunca tek akciğer ventilasyonu (TAV) sonrasında 30 dakika süreyle çift akciğer ventilasyonu (ÇAV) uygulandı. Karnozin grubundaki sıçanlara deneye başlamadan 10 dakika önce 250mg/kg dozunda intraperitoneal karnozin enjeksiyonu, kontrol grubuna ise aynı miktarda Serum Fizyolojik enjeksiyonu yapıldı. Her iki gruptanda TAV ve ÇAV sonunda biyokimyasal analiz ve histopatolojik inceleme için akciğer doku örnekleri alındı. Biyokimyasal analizde doku superoksid dismutaz (SOD), malondialdehit (MDA) ve tümör nekroz faktör alfa (TNF-α) düzeyleri ölçüldü. Histopatolojik incelemede dokular hemotoksilen eosin ile boyandı ve akciğer hasar parametreleri, alveolar konjesyon, intraalveoler kanama, lökosit ve lenfosit infiltrasyonu varlığı ve miktarı ile skorlandı. Bulgular: Karnozin uygulanan grupta, TAV ve ÇAV sonunda TNF-α düzeylerinde kontrol grubuna kıyasla istatistiksel olarak anlamlı bir azalma saptandı (p<0,05). Ancak her iki grupta da TAV ve ÇAV sonrası MDA ve SOD değerlerinde anlamlı bir farklılık görülmedi (p>0,05). Histopatolojik değerlendirmede, karnozin uygulanan grupta polimorfonükleer lökosit (PMNL) ve lenfosit infiltrasyonunun istatiksel olarak anlamlı düzeyde azaldığı görüldü (p<0,05). Sonuç: Göğüs cerrahisi uygulamalarında, TAV’a bağlı akciğer hasarının önlenmesinde karnozin kullanımının potansiyel bir terapötik ajan olabileceğini düşünüyoruz.

Keywords

• Tek akciğer ventilasyonu
• karnozin
• MDA
• SOD
• TNF-α
• alveoler konjesyon
• lenfosit infiltrasyonu

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