Research Article
BibTex RIS Cite

Kırıkkale Yöresinde İnsan ve Hayvan Kaynaklarından İzole Edilen E. coli O157:H7’nin Prevalansı

Year 2018, , 256 - 262, 30.12.2018
https://doi.org/10.24938/kutfd.403266

Abstract

Amaç: Escherichia coli O157:H7 suşu
hemorajik kolite neden olmakta ve çok düşük konsantrasyonlarda bile ciddi
salgınlara yol açabilmektedir. Bulaşma kontamine yiyeceklerin fekal-oral yol
ile alınması ile gerçekleşmekte fakat doğrudan insan teması ile de
görülebilmektedir. Ülkemizde E. coli O157:H7 prevalansı genel olarak
bilinmemekle beraber Kırıkkale bölgesinde de bilinmemektedir. E.
coli O157:H7 prevalansını belirlemek amacı ile Kırıkkale bölgesinde insan,
besi hayvanı, yem ve et örnekleri bir yıl süre ile araştırıldı.

Gereç ve Yöntemler: Bütün örnekler mümkün olan
en kısa sürede doğru ve steril şartlarda laboratuvara getirildi. E.
coli O157:H7 izolasyonunda 89 gastroenteritli hastanın dışkı örneği, 108
sığır dışkısı, 69 değişik hayvan yemi ve 84 et (kıyma) örneği klasik kültürü
takiben Dynabead ve ELISA metotları kullanılarak belirlendi. Kıyma örnekleri
çalışma testleri yapılıncaya kadar -70 derecede bekletildi.

Bulgular: E. coli O157:H7 araştırılan
insan ve hayvanlarda, yem veya et (kıyma) örneklerinde tespit edilemedi.







Sonuç: Bulgularımız ahırdan insana uzanan
besin zincirinin E. coli O157:H7 suşu açısından güvenli olduğunu
göstermiştir. Öte yandan diğer besin ve su kaynakları bu mikroorganizma için
potansiyel kaynaklar olabilir.

References

  • 1. Chattaway MA, Dallman T, Okeke IN, Wain J. Enteroaggregative E. coli O104 from an outbreak of HUS in Germany 2011, could it happen again? J Infect Dev Ctries. 2011;5(6):425-36.
  • 2. Rubino S, Capuccinelli P, Kelvin DJ. Escherichia coli (STEC) serotype O104 outbreak causing haemolytic syndrome (HUS) in Germany and France. J Infect Dev Ctries. 2011;5(6):437-40.
  • 3. Riley LW, Remis RS, Helgerson SD, McGee HB, Wells JG, Davis BR et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983;308(12):681-5.
  • 4. Riley LW. The epidemiological, clinical and microbiological features of hemorrhagic colitis. Ann Rev Microbiol. 1987;41:383-407.
  • 5. Jackson SA, Patel IR, Barnaba T, LeClerc JE, Cebula TA. Investigating the global genomic diversity of Escherichia coli using a multi-genome DNA microarray platform with novel gene prediction strategy. BMC Genomics. 2011;12:349.
  • 6. Ravva SV, Sarreal CZ, Mandrell RE. Identification of protozoa in dairy lagoon wastewater that consume Escherichia coli O157:H7 preferentially. PLoS One. 2010;5:e15671.
  • 7. Griffin PM, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic Escherichia coli and associated hemolytic uremic syndrome. Am J Epidemiol. 1991;13:60-98.
  • 8. Bopp CA, Brenner FW, Wells JG, Strockbine AA. Escherichia, Shigella and Salmonella. In: Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH, eds. Manual of Clinical Microbiology. 7th ed. Washington D.C. AMS Press, 2003:654-71.
  • 9. Karmali MA, Petric M, Lim C, Flemming PC, Arbus GS, Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin producing Escherichia coli. J Infect Dis. 1985;151(5):775-82.
  • 10. Hascelik G, Akan OA, Diker S, Baykal M. Campylobacter and Enterohemorrhagic Escherichia coli associated gastroenteritis in Turkish children. J Diarrheal Dis Res Dec. 1991;9(4):315-7.
  • 11. Amisano G, Fornasero S, Migliaretti G, Caramello S, Tarasco V, Savino F. Diarrheagenic Escherichia coli in acute gastroenteritis in infants in north-west Italy. New Microbiologica. 2011;34(1):45-51.
  • 12. Käppeli U, Hächler H, Giezendanner N, Beutin L, Stephan R. Human infection with non-O157 shiga toxin-producing Escherichia coli, Switzerland, 2000-2009. Emerg Infect Dis. 2011;17(2):180-5.
  • 13. Chigor VN, Umoh VJ, Smith SI, Igbinosa EO, Okoh AI. Multidrug resistance and plasmid patterns of Escherichia coli O157 and other E. coli isolated from diarrhoeal stools and surface waters from some selected sources in Zaria, Nigeria. Int J Environ Res Public Health. 2010;7(10):3831-41. 14. Inat G, Siriken B. Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immune-magnetic separation technique and stx1and stx2 genes by multiplex PCR in slaughtered cattle in Samsun province, Turkey. J Vet Sci. 2010;11(4):321-6.
  • 15. Ahmed AM, Shimamoto T. Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products. Int J Food Microbiol. 2015;193:68-73.
  • 16. Hessain AM, Al-Arfaj AA, Zakri AM, El-Jakee JK, Al-Zogibi OG, Hemeg HA et al. Molecular characterization of Escherichia coli O157:H7 recovered from meat and meat products relevant to human health in Riyadh, Saudi Arabia. Saudi J Biol Sci. 2015;22(6):725-9.
  • 17. Abdissa R, Haile W, Fite AT, Beyi AF, Agga GE, Edao BM et al. Prevalence of Escherichia coli O157:H7 in beef cattle at slaughter and beef carcasses at retail shops in Ethiopia. BMC Infect Dis. 2017;17(1):277.
  • 18. Chapman PA, Siddons CA, Cerdan Malo AT, Harkin MA. A 1 year study of Escherichia coli O157 in raw beef and lamb products. Epidemiol Infect. 2000;124(2):207-13.
  • 19. Yin X, Zhu J, Feng Y, Chambers JR, Gong J, Gyles CL. Differential gene expression and adherence of Escherichia coli O157:H7 in vitro and in ligated pig intestines. PLoS One. 2011;6(2):e17424.
  • 20. Easton DM, Totsika M, Allsopp LP, Phan MD, Idris A, Wurpel DJ et al. Characterization of EHaJ, a new autotransporter protein from enterohaemorrhagic and enteropathogenic Escherichia coli. Front Microbiol. 2011;2:e120.
  • 21. Sperandio V. Sdi A sensing of acyl-homoserine lactones by enterohemorrhagic E. coli (EHEC) serotype O157:H7 in the bovine rumen. Gut microbes. 2010;1(6):432-5.

PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE

Year 2018, , 256 - 262, 30.12.2018
https://doi.org/10.24938/kutfd.403266

Abstract

Objective: Escherichia
coli O157:H7 strain is a cause of hemorrhagic colitis and may give rise to
severe outbreaks even at a low concentration. Transmission may occur through
fecal-oral route with contaminated food but direct transmission from personal
contact is also possible. Presence of E.
coli O157:H7 was investigated in humans, cattle, animal feed, and ground
beef over a one-year year period in order to determine the prevalence in the
Kırıkkale region.

Material and Methods: All samples were transferred to the
microbiology laboratory as rapidly as possible under appropriate and sterile
conditions. The isolation of E. coli O157:H7
was performed by serotyping with Dynabeads and ELISA methods in stool specimens
in 89 patients with gastroenteritis, 108 cattle, 69 different animal broth
samples, and 84 samples from ground beef after culture using classical methods.
Minced meat
samples were kept at -70 degrees until working tests.

Results: E. coli O157:H7
was not detected in humans or animals or sources including animal feed and beef
carcasses.







Conclusion: Our results indicate that the meat chain from
cattle to humans is safe with respect to the
E. coli O157:H7 strain. On the other hand, other food or water
sources may be potential sources for this microorganism.

References

  • 1. Chattaway MA, Dallman T, Okeke IN, Wain J. Enteroaggregative E. coli O104 from an outbreak of HUS in Germany 2011, could it happen again? J Infect Dev Ctries. 2011;5(6):425-36.
  • 2. Rubino S, Capuccinelli P, Kelvin DJ. Escherichia coli (STEC) serotype O104 outbreak causing haemolytic syndrome (HUS) in Germany and France. J Infect Dev Ctries. 2011;5(6):437-40.
  • 3. Riley LW, Remis RS, Helgerson SD, McGee HB, Wells JG, Davis BR et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983;308(12):681-5.
  • 4. Riley LW. The epidemiological, clinical and microbiological features of hemorrhagic colitis. Ann Rev Microbiol. 1987;41:383-407.
  • 5. Jackson SA, Patel IR, Barnaba T, LeClerc JE, Cebula TA. Investigating the global genomic diversity of Escherichia coli using a multi-genome DNA microarray platform with novel gene prediction strategy. BMC Genomics. 2011;12:349.
  • 6. Ravva SV, Sarreal CZ, Mandrell RE. Identification of protozoa in dairy lagoon wastewater that consume Escherichia coli O157:H7 preferentially. PLoS One. 2010;5:e15671.
  • 7. Griffin PM, Tauxe RV. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic Escherichia coli and associated hemolytic uremic syndrome. Am J Epidemiol. 1991;13:60-98.
  • 8. Bopp CA, Brenner FW, Wells JG, Strockbine AA. Escherichia, Shigella and Salmonella. In: Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH, eds. Manual of Clinical Microbiology. 7th ed. Washington D.C. AMS Press, 2003:654-71.
  • 9. Karmali MA, Petric M, Lim C, Flemming PC, Arbus GS, Lior H. The association between idiopathic hemolytic uremic syndrome and infection by verotoxin producing Escherichia coli. J Infect Dis. 1985;151(5):775-82.
  • 10. Hascelik G, Akan OA, Diker S, Baykal M. Campylobacter and Enterohemorrhagic Escherichia coli associated gastroenteritis in Turkish children. J Diarrheal Dis Res Dec. 1991;9(4):315-7.
  • 11. Amisano G, Fornasero S, Migliaretti G, Caramello S, Tarasco V, Savino F. Diarrheagenic Escherichia coli in acute gastroenteritis in infants in north-west Italy. New Microbiologica. 2011;34(1):45-51.
  • 12. Käppeli U, Hächler H, Giezendanner N, Beutin L, Stephan R. Human infection with non-O157 shiga toxin-producing Escherichia coli, Switzerland, 2000-2009. Emerg Infect Dis. 2011;17(2):180-5.
  • 13. Chigor VN, Umoh VJ, Smith SI, Igbinosa EO, Okoh AI. Multidrug resistance and plasmid patterns of Escherichia coli O157 and other E. coli isolated from diarrhoeal stools and surface waters from some selected sources in Zaria, Nigeria. Int J Environ Res Public Health. 2010;7(10):3831-41. 14. Inat G, Siriken B. Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immune-magnetic separation technique and stx1and stx2 genes by multiplex PCR in slaughtered cattle in Samsun province, Turkey. J Vet Sci. 2010;11(4):321-6.
  • 15. Ahmed AM, Shimamoto T. Molecular analysis of multidrug resistance in Shiga toxin-producing Escherichia coli O157:H7 isolated from meat and dairy products. Int J Food Microbiol. 2015;193:68-73.
  • 16. Hessain AM, Al-Arfaj AA, Zakri AM, El-Jakee JK, Al-Zogibi OG, Hemeg HA et al. Molecular characterization of Escherichia coli O157:H7 recovered from meat and meat products relevant to human health in Riyadh, Saudi Arabia. Saudi J Biol Sci. 2015;22(6):725-9.
  • 17. Abdissa R, Haile W, Fite AT, Beyi AF, Agga GE, Edao BM et al. Prevalence of Escherichia coli O157:H7 in beef cattle at slaughter and beef carcasses at retail shops in Ethiopia. BMC Infect Dis. 2017;17(1):277.
  • 18. Chapman PA, Siddons CA, Cerdan Malo AT, Harkin MA. A 1 year study of Escherichia coli O157 in raw beef and lamb products. Epidemiol Infect. 2000;124(2):207-13.
  • 19. Yin X, Zhu J, Feng Y, Chambers JR, Gong J, Gyles CL. Differential gene expression and adherence of Escherichia coli O157:H7 in vitro and in ligated pig intestines. PLoS One. 2011;6(2):e17424.
  • 20. Easton DM, Totsika M, Allsopp LP, Phan MD, Idris A, Wurpel DJ et al. Characterization of EHaJ, a new autotransporter protein from enterohaemorrhagic and enteropathogenic Escherichia coli. Front Microbiol. 2011;2:e120.
  • 21. Sperandio V. Sdi A sensing of acyl-homoserine lactones by enterohemorrhagic E. coli (EHEC) serotype O157:H7 in the bovine rumen. Gut microbes. 2010;1(6):432-5.
There are 20 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Teoman Zafer Apan 0000-0002-3243-0916

Murat Yıldırım This is me

Mehmet Başalan

Aylin Kasımoğlu

Publication Date December 30, 2018
Submission Date March 8, 2018
Published in Issue Year 2018

Cite

APA Apan, T. Z., Yıldırım, M., Başalan, M., Kasımoğlu, A. (2018). PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE. The Journal of Kırıkkale University Faculty of Medicine, 20(3), 256-262. https://doi.org/10.24938/kutfd.403266
AMA Apan TZ, Yıldırım M, Başalan M, Kasımoğlu A. PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE. Kırıkkale Üni Tıp Derg. December 2018;20(3):256-262. doi:10.24938/kutfd.403266
Chicago Apan, Teoman Zafer, Murat Yıldırım, Mehmet Başalan, and Aylin Kasımoğlu. “PREVALENCE OF E. Coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE”. The Journal of Kırıkkale University Faculty of Medicine 20, no. 3 (December 2018): 256-62. https://doi.org/10.24938/kutfd.403266.
EndNote Apan TZ, Yıldırım M, Başalan M, Kasımoğlu A (December 1, 2018) PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE. The Journal of Kırıkkale University Faculty of Medicine 20 3 256–262.
IEEE T. Z. Apan, M. Yıldırım, M. Başalan, and A. Kasımoğlu, “PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE”, Kırıkkale Üni Tıp Derg, vol. 20, no. 3, pp. 256–262, 2018, doi: 10.24938/kutfd.403266.
ISNAD Apan, Teoman Zafer et al. “PREVALENCE OF E. Coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE”. The Journal of Kırıkkale University Faculty of Medicine 20/3 (December 2018), 256-262. https://doi.org/10.24938/kutfd.403266.
JAMA Apan TZ, Yıldırım M, Başalan M, Kasımoğlu A. PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE. Kırıkkale Üni Tıp Derg. 2018;20:256–262.
MLA Apan, Teoman Zafer et al. “PREVALENCE OF E. Coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE”. The Journal of Kırıkkale University Faculty of Medicine, vol. 20, no. 3, 2018, pp. 256-62, doi:10.24938/kutfd.403266.
Vancouver Apan TZ, Yıldırım M, Başalan M, Kasımoğlu A. PREVALENCE OF E. coli O157:H7 ISOLATED FROM HUMAN AND ANIMAL SOURCE IN KIRIKKALE PROVINCE. Kırıkkale Üni Tıp Derg. 2018;20(3):256-62.

Bu Dergi, Kırıkkale Üniversitesi Tıp Fakültesi Yayınıdır.