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Investigation of The Repeatability Performance of Erythrocyte Sedimentation Rate Test

Year 2020, , 153 - 158, 31.08.2020
https://doi.org/10.24938/kutfd.630573

Abstract

Objective: In laboratories, the erythrocyte sedimentation rate (ESR) test may need to be repeated for various reasons. The aim of this study was to evaluate the repeatability performance of the ESR test by comparing the ESR values measured after different mixing procedures following the first measurement in sedimentation tubes.
Material and Methods: The sedimentation tubes of 37 samples, which were brought to our clinical biochemistry laboratory for analysis on the same day, were first inverted very lightly and only once after the first measurement, and the second reading was performed. Then, the third reading was performed by inverting the tubes thoroughly and repeatedly.
Results: While the ICC (intraclass correlation coefficient) value between the first and second measurements was 0.868, the ICC value between the first and third measurements was 0.927. In the third measurement, there was a better agreement with the first measurement compared to the second measurement.
Conclusion: In cases where the ESR assay needs to be repeated, sedimentation tubes should be inverted thoroughly and repeatedly. However, despite this application, the compatibility between measurements may not be at the desired level.

References

  • 1. Markanday A. Acute phase reactants in infections: evidence-based review and a guide for clinicians. Open Forum Infect Dis. 2015;2(3):ofv098. Doi:10.1093/ofid/ofv098.
  • 2. Bray C, Bell LN, Liang H, Haykal R, Kaiksow F, Mazza JJ et al. Erythrocyte sedimentation rate and C-reactive protein measurements and their relevance in clinical medicine. WMJ. 2016;115(6):317-21.
  • 3. Erdoğdu Hİ, Atalay E. Relationship between high erythrocyte sedimentation rate and 25-hydroxy vitamin D and C-reactive protein levels. Sakarya Med J. 2018;8(4):798-805.
  • 4. Tunç S, Atagün MI, Neşelioğlu S, Bilgin YY, Başbuğ HS, Erel Ö. Ischemia-modified albumin: a unique marker of global metabolic risk in schizophrenia and mood disorders. Psychiatry and clinical psychopharmacology. 2019;29(2):123-9. Doi:10.1080/24750573.2018.1517466.
  • 5. Fabry TL. Mechanism of erythrocyte aggregation and sedimentation. Blood. 1987;70(5):1572-6.
  • 6. Reinhart WH. Erythrocyte sedimentation rate-more than an old fashion? Ther Umsch. 2006;63(1):108-12. Doi:10.1024/0040-5930.63.1.108.
  • 7. Lapic I, Piva E, Spolaore F, Tosato F, Pelloso M, Plebani M. Automated measurement of the erythrocyte sedimentation rate: method validation and comparison. Clin Chem Lab Med. 2019;57(9):1364-73. Doi:10.1515/cclm-2019-0204.
  • 8. Landaas S, Juell A. The quality of laboratory analyses in the primary health care. A proficiency survey on hemoglobin, glucose, leucocyte count and sedimentation rate (ESR) measurements. Scand J Prim Health Care. 1986;4(3):169-73. Doi:10.3109/02813438609014825
  • 9. Dinant GJ, Knottnerus JA, van Wersch JW, Dolhain JP. Erythrocyte sedimentation; a study of the reproducibility of this test in family practice. Ned Tijdschr Geneeskd. 1988;132(15):680-4.
  • 10. Siersema PD, van Eijk HG. The erythrocyte sedimentation rate; a study of the reproducibility of this determination in family practice. Ned Tijdschr Geneeskd. 1988;132(24):1126-7.
  • 11. Dinant GJ, Knottnerus JA, van Aubel PG, van Wersch JW. Reliability of the erythrocyte sedimentation rate in general practice. Scand J Prim Health Care. 1989;7(4):231-5.
  • 12. Dinant GJ, Knottnerus JA, Van Wersch JW. Blood sample transportation and the erythrocyte sedimentation rate. J R Coll Gen Pract. 1989;39(324):302.
  • 13. Lapic I, Piva E, Spolaore F, Musso G, Tosato F, Pelloso M et al. Ves-Matic CUBE 200: is modified Westergren method for erythrocyte sedimentation rate a valid alternative to the gold standard? J Clin Pathol. 2019;72(10):716-9. Doi:10.1136/jclinpath-2019-205873.

ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI

Year 2020, , 153 - 158, 31.08.2020
https://doi.org/10.24938/kutfd.630573

Abstract

Amaç: Laboratuvarlarda, eritrosit sedimantasyon hızı (ESR) testinin çeşitli sebeplerle tekrarlanması gerekebilmektedir. Bu çalışmanın amacı, sedimantasyon tüplerinde ilk ölçümü takiben yapılan farklı altüst etme işlemlerinden sonra ölçülen ESR değerlerini karşılaştırarak, ESR testinin tekrarlanabilirlik performansını değerlendirmektir.
Gereç ve Yöntemler: Klinik biyokimya laboratuvarımıza aynı gün analiz için gelen 37 numuneye ait sedimantasyon tüpleri, ilk ölçümü takiben, önce çok hafifçe ve bir defaya mahsus olmak üzere altüst edildi ve ikinci okuma yapıldı. Ardından, tüpler iyice ve defalarca altüst edilerek üçüncü okuma gerçekleştirildi.
Bulgular: İlk ve ikinci ölçüm arasındaki ICC (sınıf içi korelasyon katsayısı) değeri 0.868; ilk ve üçüncü ölçüm arasındaki ICC değeri ise 0.927 idi. Üçüncü ölçümlerde, ikinci ölçümlerdekine göre, ilk ölçümle daha iyi bir uyum vardı.
Sonuç: ESR tahlilinin tekrar çalışılması gereken durumlarda, sedimantasyon tüpleri iyice ve birkaç defa altüst edilmelidir. Bununla birlikte, söz konusu uygulamaya rağmen, ölçümler arasındaki uyum istenen seviyede olmayabilir.

References

  • 1. Markanday A. Acute phase reactants in infections: evidence-based review and a guide for clinicians. Open Forum Infect Dis. 2015;2(3):ofv098. Doi:10.1093/ofid/ofv098.
  • 2. Bray C, Bell LN, Liang H, Haykal R, Kaiksow F, Mazza JJ et al. Erythrocyte sedimentation rate and C-reactive protein measurements and their relevance in clinical medicine. WMJ. 2016;115(6):317-21.
  • 3. Erdoğdu Hİ, Atalay E. Relationship between high erythrocyte sedimentation rate and 25-hydroxy vitamin D and C-reactive protein levels. Sakarya Med J. 2018;8(4):798-805.
  • 4. Tunç S, Atagün MI, Neşelioğlu S, Bilgin YY, Başbuğ HS, Erel Ö. Ischemia-modified albumin: a unique marker of global metabolic risk in schizophrenia and mood disorders. Psychiatry and clinical psychopharmacology. 2019;29(2):123-9. Doi:10.1080/24750573.2018.1517466.
  • 5. Fabry TL. Mechanism of erythrocyte aggregation and sedimentation. Blood. 1987;70(5):1572-6.
  • 6. Reinhart WH. Erythrocyte sedimentation rate-more than an old fashion? Ther Umsch. 2006;63(1):108-12. Doi:10.1024/0040-5930.63.1.108.
  • 7. Lapic I, Piva E, Spolaore F, Tosato F, Pelloso M, Plebani M. Automated measurement of the erythrocyte sedimentation rate: method validation and comparison. Clin Chem Lab Med. 2019;57(9):1364-73. Doi:10.1515/cclm-2019-0204.
  • 8. Landaas S, Juell A. The quality of laboratory analyses in the primary health care. A proficiency survey on hemoglobin, glucose, leucocyte count and sedimentation rate (ESR) measurements. Scand J Prim Health Care. 1986;4(3):169-73. Doi:10.3109/02813438609014825
  • 9. Dinant GJ, Knottnerus JA, van Wersch JW, Dolhain JP. Erythrocyte sedimentation; a study of the reproducibility of this test in family practice. Ned Tijdschr Geneeskd. 1988;132(15):680-4.
  • 10. Siersema PD, van Eijk HG. The erythrocyte sedimentation rate; a study of the reproducibility of this determination in family practice. Ned Tijdschr Geneeskd. 1988;132(24):1126-7.
  • 11. Dinant GJ, Knottnerus JA, van Aubel PG, van Wersch JW. Reliability of the erythrocyte sedimentation rate in general practice. Scand J Prim Health Care. 1989;7(4):231-5.
  • 12. Dinant GJ, Knottnerus JA, Van Wersch JW. Blood sample transportation and the erythrocyte sedimentation rate. J R Coll Gen Pract. 1989;39(324):302.
  • 13. Lapic I, Piva E, Spolaore F, Musso G, Tosato F, Pelloso M et al. Ves-Matic CUBE 200: is modified Westergren method for erythrocyte sedimentation rate a valid alternative to the gold standard? J Clin Pathol. 2019;72(10):716-9. Doi:10.1136/jclinpath-2019-205873.
There are 13 citations in total.

Details

Primary Language Turkish
Subjects Health Care Administration
Journal Section Articles
Authors

Fatih Kara 0000-0002-9729-5380

Ergin Taşkın 0000-0002-1883-6055

Seda Çelik 0000-0003-2055-3537

Duygu Mine Yavuz This is me 0000-0002-0425-0766

Veysel Tahiroğlu 0000-0003-3516-5561

Publication Date August 31, 2020
Submission Date October 7, 2019
Published in Issue Year 2020

Cite

APA Kara, F., Taşkın, E., Çelik, S., Yavuz, D. M., et al. (2020). ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI. The Journal of Kırıkkale University Faculty of Medicine, 22(2), 153-158. https://doi.org/10.24938/kutfd.630573
AMA Kara F, Taşkın E, Çelik S, Yavuz DM, Tahiroğlu V. ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI. Kırıkkale Üni Tıp Derg. August 2020;22(2):153-158. doi:10.24938/kutfd.630573
Chicago Kara, Fatih, Ergin Taşkın, Seda Çelik, Duygu Mine Yavuz, and Veysel Tahiroğlu. “ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI”. The Journal of Kırıkkale University Faculty of Medicine 22, no. 2 (August 2020): 153-58. https://doi.org/10.24938/kutfd.630573.
EndNote Kara F, Taşkın E, Çelik S, Yavuz DM, Tahiroğlu V (August 1, 2020) ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI. The Journal of Kırıkkale University Faculty of Medicine 22 2 153–158.
IEEE F. Kara, E. Taşkın, S. Çelik, D. M. Yavuz, and V. Tahiroğlu, “ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI”, Kırıkkale Üni Tıp Derg, vol. 22, no. 2, pp. 153–158, 2020, doi: 10.24938/kutfd.630573.
ISNAD Kara, Fatih et al. “ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI”. The Journal of Kırıkkale University Faculty of Medicine 22/2 (August 2020), 153-158. https://doi.org/10.24938/kutfd.630573.
JAMA Kara F, Taşkın E, Çelik S, Yavuz DM, Tahiroğlu V. ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI. Kırıkkale Üni Tıp Derg. 2020;22:153–158.
MLA Kara, Fatih et al. “ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI”. The Journal of Kırıkkale University Faculty of Medicine, vol. 22, no. 2, 2020, pp. 153-8, doi:10.24938/kutfd.630573.
Vancouver Kara F, Taşkın E, Çelik S, Yavuz DM, Tahiroğlu V. ERİTROSİT SEDİMANTASYON HIZI TESTİNİN TEKRARLANABİLİRLİK PERFORMANSININ ARAŞTIRILMASI. Kırıkkale Üni Tıp Derg. 2020;22(2):153-8.

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