COEXISTENCE OF CD44 AND KI-67 AS THE PROGNOSTIC MARKERS IN RENAL CELL CARCINOMA

Abstract

Objective: An important feature of renal cell carcinoma (RCC) is its changeable prognosis. In recent years, several potential biomarkers have been investigated and confirmatory studies of these promising biomarkers are necessary to improve the clinical practice. In the present study, it was aimed to investigate the prognostic significance of CD44 expression by comparing it with Ki-67 and p53 in clear cell RCC.

Material and Methods: Formalin- fixed, paraffin-embedded tissue sections of clear cell RCC from 34 patients, who had undergone radical or partial nephrectomy, were included in this study. Correlations between CD44, Ki-67 and p53 immunohistochemical expressions and clinicopathological parameters were determined.

Results: CD44-high expression group (HEG) was significantly associated (p=0.035) and correlated (p=0.016, r=0.41) with a high nuclear grade. Similarly, the Ki-67-HEG was significantly associated (p=0.01) and correlated (p=0.01, r=0.436) with a high nuclear grade. We also found significant association (p<0.01) and correlation (p<0.01, r=0.621) between expressions of these markers. However, no antigen-antibody interaction was detected by p53 staining in clear cell RCC tissue sections.

Conclusion: We concluded that increased expressions of CD44 and Ki-67 in tumour tissues predict an aggressive course of RCC patients.

Keywords

• Renal cell carcinoma,clear cell,CD44,Ki-67,p53

Renal Hücreli Karsinomda Prognostik Belirteçler Olarak CD44 ve Ki-67 Birlikteliği

Abstract

Amaç: Renal hücreli karsinomun (RHK) önemli bir özelliği değişen prognozudur. Son yıllarda, birkaç potansiyel biyobelirteç araştırılmış olup ve bu umut verici biyobelirteçlere ait doğrulayıcı çalışmalar klinik uygulamayı geliştirmek için gereklidir. Bu çalışmada, berrak hücreli RHK'de CD44 ekspresyonunun prognostik öneminin, Ki-67 ve p53 ile karşılaştırarak araştırılması amaçlandı.

Gereç ve Yöntemler: Radikal veya parsiyel nefrektomi yapılan 34 hastanın berrak hücreli RHK'nin formalinle fikse edilmiş, parafine gömülü doku kesitleri çalışmaya dahil edildi. CD44, Ki-67 ve p53 immünohistokimyasal ekspresyonları ve klinikopatolojik parametreler arasındaki korelasyonlar belirlendi.

Bulgular: CD44-yüksek ekspresyon grubu (HEG) ile yüksek nükleer derece arasında anlamlı bir ilişki (p=0.035) ve korelasyon saptandı (p=0.016, r = 0.41). Benzer şekilde, Ki-67-HEG ile yüksek nükleer derece arasında önemli bir ilişki (p=0.01) ve korelasyon saptandı (p=0.01, r=0.436). Ayrıca bu iki belirteç arasında anlamlı ilişki (p<0.01) ve korelasyon (p<0.01, r=0.621) bulundu. Bununla birlikte, berrak hücreli RHK doku kesitlerinde p53 immünhistokimyasal boyanması saptanmadı.

Sonuç: Tümör dokularında artmış CD44 ve Ki-67 ekspresyonlarının, RHK hastalarında agresif bir seyiri öngördüğü sonucuna vardık.

Keywords

• Renal hücreli karsinom,berrak hücreli,CD44,Ki-67,p53,p53

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