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Mavi Calopo (Calopogonium caeruleum) Tohumlarının Çimlenmesinin İyileştirilmesi

Year 2025, Volume: 5 Issue: 2, 66 - 75, 31.12.2025

Abstract

Calopogonium caeruleum, Endonezya’daki plantasyonlarda yaygın olarak yem bitkisi ve toprak örtüsü olarak kullanılan baklagil bir bitkidir. Ayrıca, verimsiz (marjinal) arazilerde toprak örtüsü olarak kullanılabilecek umut verici bir seçenek olarak değerlendirilmektedir. Ancak, bu bitkinin yetiştirilmesi, çimlenmeyi engelleyen sert bir tohum kabuğuna sahip olması nedeniyle bazı zorluklar içermektedir. Bu nedenle, çimlenmeyi artırmak amacıyla çeşitli skarifikasyon (tohum kabuğunu yumuşatma) yöntemleri uygulanmıştır. Uygulanan ön işlemler arasında; (i) %95–98 sülfürik asitle 5, 10, 15 ve 20 dakika süreyle muamele, (ii) ortam sıcaklığında %0.3 KNO₃ çözeltisinde 12, 24, 36 ve 48 saat bekletme, (iii) 70 °C sıcaklıkta gliserin ile 30, 60, 90 ve 120 dakika işlem görme, (iv) zımpara kâğıdı ile 2 ve 4 kez fiziksel aşındırma yer almaktadır. Bu işlemler arasında, 70 °C’de 120 dakika boyunca gliserin ile yapılan uygulama, C. caeruleum tohumlarında en yüksek çimlenme oranı ve tohum canlılığı sonucunu vermiştir.

References

  • Acevedo-Rodríguez P (2005). Vines and climbing plants of Puerto Rico and the Virgin Islands. Contributions from the United States National Herbarium, 51:1-483
  • Ali R M, Elfeky S S, Abbas H (2008). Response of salt stressed Ricinus communis L. to exogenous application of glycerol and/or aspartic acid. Journal of Biological Sciences 8:171-175
  • Asra R (2014). Pengaruh hormon giberelin (GA3) terhadap daya kecambah dan vigoritas Calopogonium caeruleum. Biospecies 7(1): 29-33
  • Atalay E, Yetim E, Soylu S, Sade B, Yorgancılar M. (2011). Farklı priming uygulamalarının ekmeklik buğday çeşitlerinde çimlenmenin başlangıç dönemdeki etkinliği. Türkiye IV. Tohumculuk Kongresi, Samsun, Turkiye, 535-539
  • Bewley J D ve Black M (1994). Seeds: Physiology of Development and Germination. Plenum Press, New York Bewley J D, Bradford K J, Hilhorst H W M, Nonogaki H (2013). Seeds: Physiology of Development, Germination and Dormancy (3rd ed.). Springer, New York
  • Bonner F T (2008). Storage of seeds. The woody plant seed manual, In: F T Bonner, R P Karrfalt & R G Nisley (Eds.), 727, USDA, New York, pp. 86-95.
  • Chai J, Ma M, Li L, Du Y (1998). Optimum moisture contents of seeds stored at ambient temperatures. Journal of Seed Science Research Supplement 1: 23-28
  • Chen C P ve Chee Y K (1992). Calopogonium caeruleum (Benth.) Sauv. Plant Resources of South-East Asia No 4: Forages. In: Mannetje, L.'t and Jones, R.M. (Eds.), PROSEA Foundation, Bogor, Indonesia. Erişim tarihi: Eylül, 3, 2025 https://prosea.prota4u.org/view.aspx?id=1850
  • Cook B G, Pengelly B C, Brown S D, Donnelly J L, Eagles D A, Franco M A, Hanson J, Partridge I J, Peter M, Schultze-Kraft R (2005). Tropical Forages: an interactive selection tool. Brisbane, Australia: CSIRO, DPI&F, CIAT, ILRI. http://www.tropicalforages.info/
  • de Morais L F, Almeida C C, Deminicis B B, Padua F T, Moren M J F, Abreul J B R, Araujo R P, Nepomuceno D D (2014). Methods for breaking dormancy of seeds of tropical forage legumes. American Journal of Plant Sciences 5(13): 1831-1835
  • Duan X H, Luo F C, Zhong S, Li Q X, Xue S M, Yang G R, Huang B Z (2015). A preliminary study on causes of seed dormancy of Brachiaria decumbens. Grassl. Turf. 35: 49-52
  • Elçi G (1988). Ziraatte baklagiller. Ankara Üniversitesi Basımevi, Ankara. (Türkçeye Çeviren)
  • Gashi B, Abdullai K, Mata V, Kongjika E (2012). Effect of gibberellic acid and potassium nitrate on seed germination of the resurrection plants Ramonda serbica and Ramonda nathaliae. African Journal of Biotechnology 11(20): 4537-4542
  • Ghive D V, Barabde N P, Pote S R (2007). Seed viability and factors affecting seed storage. Asian Journal of Bio Science 2(1/2): 205-208
  • Gupta P C (1993). Seed Vigour Testing, Handbook of Seed Testing. In: P K Agrawal (Ed.), Ministry of Agriculture, India, 242-249
  • Hassen A, Pieterse P P, Rethman N F G (2004). Effect of pre-planting seed treatment on dormancy breaking and germination of Indigofera accessions. Tropical Grasslands 38(3):154-157
  • ISTA (1966). International Rules for Seed Testing. Proceedings for International Seed Testing Association 31: 1-152
  • ISTA (2003). International Rules for Seed Testing. International Seed Testing Association, Zurich
  • ISTA (2007). International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland
  • Jarrar H, El‐Keblawy A, Albawab M, Ghenai C ve Sheteiwy M (2024). Seed priming as a promising technique for sustainable restoration of dryland. Restoration Ecology 32(6): e14182.
  • Magar B N, Chapagaee P ve Bohora A (2025). Evaluating the efficacy of organic and inorganic seed priming methods in promoting cucumber germination and growth. Turkish Journal of Agriculture-Food Science and Technology 13(2): 406-412
  • Maguire J D (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science 2: 176-177.
  • Middleton C H and Mellor W (1982). Grazing assessment of the tropical legume Calopogonium caeruleum. Tropical Grasslands 16(4): 213-216
  • Missanjo E, Maya C, Kapira D, Banda H, Thole G K (2013). Effect of seed size and pretreatment method on germination of Albizia lebbeck. International Scholarly Research Notices 2013(1): 969026
  • Olatunji D, Maku O J, Odumefun O P (2013). The effect of pre-treatment on germination and early seedling growth of Acacia auriculiformis Cunn Ex. Benth. African Journal of Plant Science 7(8): 325-330
  • Rao I M and Northup B K (2009). Potential for legume cover crops in tropical agriculture. In M R Jahn and A M Driessen (Eds.), Tropical Agroecosystem, CABI Publishing, pp. 149-168
  • Rusdy M (2015). Enhancing germination in seeds of Centrosema pubescens. International Journal of Scientific and Research Publications 5(10): 1-4
  • Sarihan E O, Ipek A, Khawar K M, Atak M, Grubuz M (2005). Role of GA3 and KNO3 inimproving the frequency of seed germination in Plantago lanceolata L. Pakistan Journal of Botany, 37(4), 883-887
  • USDA-ARS (2014). Germplasm Resources Information Network (GRIN). Erişim tarihi: Eylül 2, 2024 https://npgsweb.ars-grin.gov/gringlobal/taxon/taxonomysearch.aspx
  • Waidyanatha U D S ve Ariyaratne W A (1976). Breaking dormancy in seed of cover legumes. The Rubber Research Institute of Sri Lanka 53: 8-16
  • Wang Y, Zhou E, Yao M, Xue D, Zhao N, Zhou Y, Li B, Wang K, Miao Y, Gu C, Wang X ve Wei L (2023). PEG-6000 priming improves aged soybean seed vigor via carbon metabolism, ros scavenging, hormone signaling, and lignin synthesis regulation. Agronomy 13(12):1-21.
  • Welbaum G E and Bradford K J (1991). Water relation of seed development and germination in muskmelon (Cucumis melo L.). VI. Influence of priming on germination responses to temperature and water potential during seed development. Journal of Experimental Botany 42(3): 393-399
  • Wycherley P R (1960). Seed germination of some tropical legumes. Journal of Rubber Research Institute of Malaya 16(2): 99-117

Optimizing Seed Sprouting of Blue Calopo (Calopogonium caeruleum)

Year 2025, Volume: 5 Issue: 2, 66 - 75, 31.12.2025

Abstract

Calopogonium caeruleum is a leguminous plant commonly used as forage and as ground cover in Indonesian plantations. It is also regarded as a promising option for ground cover on marginal lands. However, its cultivation faces challenges due to the presence of a hard seed coat, which hinders germination. Various scarification methods were treated to seeds to enhance germination. It is used pre-treatment are (i) using 95-98 % of sulfuric acid for 5, 10, 15 and 20 minutes, (ii) using 0.3 % of KNO3 in ambient temperature for 12, 24, 36 and 48 hours, (iii) using glycerine at 70oC for 30, 60, 90 and 120 minutes, (iv) using sandpaper with 2 and 4 times of scratch. The treatment using glycerine at 60°C for 120 minutes showed the best result of germination percentage and seed vigor on C. caeruleum seeds.

References

  • Acevedo-Rodríguez P (2005). Vines and climbing plants of Puerto Rico and the Virgin Islands. Contributions from the United States National Herbarium, 51:1-483
  • Ali R M, Elfeky S S, Abbas H (2008). Response of salt stressed Ricinus communis L. to exogenous application of glycerol and/or aspartic acid. Journal of Biological Sciences 8:171-175
  • Asra R (2014). Pengaruh hormon giberelin (GA3) terhadap daya kecambah dan vigoritas Calopogonium caeruleum. Biospecies 7(1): 29-33
  • Atalay E, Yetim E, Soylu S, Sade B, Yorgancılar M. (2011). Farklı priming uygulamalarının ekmeklik buğday çeşitlerinde çimlenmenin başlangıç dönemdeki etkinliği. Türkiye IV. Tohumculuk Kongresi, Samsun, Turkiye, 535-539
  • Bewley J D ve Black M (1994). Seeds: Physiology of Development and Germination. Plenum Press, New York Bewley J D, Bradford K J, Hilhorst H W M, Nonogaki H (2013). Seeds: Physiology of Development, Germination and Dormancy (3rd ed.). Springer, New York
  • Bonner F T (2008). Storage of seeds. The woody plant seed manual, In: F T Bonner, R P Karrfalt & R G Nisley (Eds.), 727, USDA, New York, pp. 86-95.
  • Chai J, Ma M, Li L, Du Y (1998). Optimum moisture contents of seeds stored at ambient temperatures. Journal of Seed Science Research Supplement 1: 23-28
  • Chen C P ve Chee Y K (1992). Calopogonium caeruleum (Benth.) Sauv. Plant Resources of South-East Asia No 4: Forages. In: Mannetje, L.'t and Jones, R.M. (Eds.), PROSEA Foundation, Bogor, Indonesia. Erişim tarihi: Eylül, 3, 2025 https://prosea.prota4u.org/view.aspx?id=1850
  • Cook B G, Pengelly B C, Brown S D, Donnelly J L, Eagles D A, Franco M A, Hanson J, Partridge I J, Peter M, Schultze-Kraft R (2005). Tropical Forages: an interactive selection tool. Brisbane, Australia: CSIRO, DPI&F, CIAT, ILRI. http://www.tropicalforages.info/
  • de Morais L F, Almeida C C, Deminicis B B, Padua F T, Moren M J F, Abreul J B R, Araujo R P, Nepomuceno D D (2014). Methods for breaking dormancy of seeds of tropical forage legumes. American Journal of Plant Sciences 5(13): 1831-1835
  • Duan X H, Luo F C, Zhong S, Li Q X, Xue S M, Yang G R, Huang B Z (2015). A preliminary study on causes of seed dormancy of Brachiaria decumbens. Grassl. Turf. 35: 49-52
  • Elçi G (1988). Ziraatte baklagiller. Ankara Üniversitesi Basımevi, Ankara. (Türkçeye Çeviren)
  • Gashi B, Abdullai K, Mata V, Kongjika E (2012). Effect of gibberellic acid and potassium nitrate on seed germination of the resurrection plants Ramonda serbica and Ramonda nathaliae. African Journal of Biotechnology 11(20): 4537-4542
  • Ghive D V, Barabde N P, Pote S R (2007). Seed viability and factors affecting seed storage. Asian Journal of Bio Science 2(1/2): 205-208
  • Gupta P C (1993). Seed Vigour Testing, Handbook of Seed Testing. In: P K Agrawal (Ed.), Ministry of Agriculture, India, 242-249
  • Hassen A, Pieterse P P, Rethman N F G (2004). Effect of pre-planting seed treatment on dormancy breaking and germination of Indigofera accessions. Tropical Grasslands 38(3):154-157
  • ISTA (1966). International Rules for Seed Testing. Proceedings for International Seed Testing Association 31: 1-152
  • ISTA (2003). International Rules for Seed Testing. International Seed Testing Association, Zurich
  • ISTA (2007). International Rules for Seed Testing. International Seed Testing Association, Bassersdorf, Switzerland
  • Jarrar H, El‐Keblawy A, Albawab M, Ghenai C ve Sheteiwy M (2024). Seed priming as a promising technique for sustainable restoration of dryland. Restoration Ecology 32(6): e14182.
  • Magar B N, Chapagaee P ve Bohora A (2025). Evaluating the efficacy of organic and inorganic seed priming methods in promoting cucumber germination and growth. Turkish Journal of Agriculture-Food Science and Technology 13(2): 406-412
  • Maguire J D (1962). Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science 2: 176-177.
  • Middleton C H and Mellor W (1982). Grazing assessment of the tropical legume Calopogonium caeruleum. Tropical Grasslands 16(4): 213-216
  • Missanjo E, Maya C, Kapira D, Banda H, Thole G K (2013). Effect of seed size and pretreatment method on germination of Albizia lebbeck. International Scholarly Research Notices 2013(1): 969026
  • Olatunji D, Maku O J, Odumefun O P (2013). The effect of pre-treatment on germination and early seedling growth of Acacia auriculiformis Cunn Ex. Benth. African Journal of Plant Science 7(8): 325-330
  • Rao I M and Northup B K (2009). Potential for legume cover crops in tropical agriculture. In M R Jahn and A M Driessen (Eds.), Tropical Agroecosystem, CABI Publishing, pp. 149-168
  • Rusdy M (2015). Enhancing germination in seeds of Centrosema pubescens. International Journal of Scientific and Research Publications 5(10): 1-4
  • Sarihan E O, Ipek A, Khawar K M, Atak M, Grubuz M (2005). Role of GA3 and KNO3 inimproving the frequency of seed germination in Plantago lanceolata L. Pakistan Journal of Botany, 37(4), 883-887
  • USDA-ARS (2014). Germplasm Resources Information Network (GRIN). Erişim tarihi: Eylül 2, 2024 https://npgsweb.ars-grin.gov/gringlobal/taxon/taxonomysearch.aspx
  • Waidyanatha U D S ve Ariyaratne W A (1976). Breaking dormancy in seed of cover legumes. The Rubber Research Institute of Sri Lanka 53: 8-16
  • Wang Y, Zhou E, Yao M, Xue D, Zhao N, Zhou Y, Li B, Wang K, Miao Y, Gu C, Wang X ve Wei L (2023). PEG-6000 priming improves aged soybean seed vigor via carbon metabolism, ros scavenging, hormone signaling, and lignin synthesis regulation. Agronomy 13(12):1-21.
  • Welbaum G E and Bradford K J (1991). Water relation of seed development and germination in muskmelon (Cucumis melo L.). VI. Influence of priming on germination responses to temperature and water potential during seed development. Journal of Experimental Botany 42(3): 393-399
  • Wycherley P R (1960). Seed germination of some tropical legumes. Journal of Rubber Research Institute of Malaya 16(2): 99-117
There are 33 citations in total.

Details

Primary Language English
Subjects Agronomy
Journal Section Research Article
Authors

Siti Maesaroh 0000-0003-1024-284X

Nurdan Şahin Demirbağ 0000-0003-2173-2393

Çiğdem Alev Özel 0000-0002-5952-1412

Submission Date July 26, 2025
Acceptance Date August 28, 2025
Publication Date December 31, 2025
Published in Issue Year 2025 Volume: 5 Issue: 2

Cite

APA Maesaroh, S., Şahin Demirbağ, N., & Özel, Ç. A. (2025). Optimizing Seed Sprouting of Blue Calopo (Calopogonium caeruleum). Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi, 5(2), 66-75.
AMA Maesaroh S, Şahin Demirbağ N, Özel ÇA. Optimizing Seed Sprouting of Blue Calopo (Calopogonium caeruleum). KUZFAD. December 2025;5(2):66-75.
Chicago Maesaroh, Siti, Nurdan Şahin Demirbağ, and Çiğdem Alev Özel. “Optimizing Seed Sprouting of Blue Calopo (Calopogonium Caeruleum)”. Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi 5, no. 2 (December 2025): 66-75.
EndNote Maesaroh S, Şahin Demirbağ N, Özel ÇA (December 1, 2025) Optimizing Seed Sprouting of Blue Calopo (Calopogonium caeruleum). Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi 5 2 66–75.
IEEE S. Maesaroh, N. Şahin Demirbağ, and Ç. A. Özel, “Optimizing Seed Sprouting of Blue Calopo (Calopogonium caeruleum)”, KUZFAD, vol. 5, no. 2, pp. 66–75, 2025.
ISNAD Maesaroh, Siti et al. “Optimizing Seed Sprouting of Blue Calopo (Calopogonium Caeruleum)”. Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi 5/2 (December2025), 66-75.
JAMA Maesaroh S, Şahin Demirbağ N, Özel ÇA. Optimizing Seed Sprouting of Blue Calopo (Calopogonium caeruleum). KUZFAD. 2025;5:66–75.
MLA Maesaroh, Siti et al. “Optimizing Seed Sprouting of Blue Calopo (Calopogonium Caeruleum)”. Kırşehir Ahi Evran Üniversitesi Ziraat Fakültesi Dergisi, vol. 5, no. 2, 2025, pp. 66-75.
Vancouver Maesaroh S, Şahin Demirbağ N, Özel ÇA. Optimizing Seed Sprouting of Blue Calopo (Calopogonium caeruleum). KUZFAD. 2025;5(2):66-75.