The Comparison of Clinical Examination, Endometrial Cytology, Biopsy and Microbiology Examinaiton Results in the Diagnosis of Endometritis in Cows

Abstract

In this research the accuracy of endometrial cytology in the diagnosis of endometritis in cattle was investigated by the comparison of the techniques routinely used. For this purpose, 13 cattle having acute endometritis, 17 having chronic endometritis and 10 healthy holstein cow was used. The cows in this research recieved clinical, micobiological, histopathological and cytological examinations. Eight of 13 cattle in acute endometritis group had no abnormal vaginal discharge but abnormal vaginal discharge was observed on 5 of the acute endometritis cases. No abnormal vaginal discharge was determined in both healthy and chronic endometritis group. There was no difference on the rectal palpation results of all the three groups. A.pyogenes, E. coli, Streptococcus spp., Staphylococcus spp., K. Pneumonia and yeast were isolated in 10 of the acute and 8 of the chronic group cattle. In a total of 12 cases in both acute and chronic groups, there was no bacterial growth. In the acute endometritis group degeneration, desquamation and neutrophyl leucocyte infiltration, edema and haemorrhage was seen in the lamina epithelialis, in the lamina propria and desquamated epithelia of the glandular tissue and neutrophyl leucocyte infiltration in the lumen of the glands and neutrophl leucocyte infiltration in the uterine lumen was observed. In the chronic cases; mononucleer cell infilration, increase of connective tissue was observed. In cytological examinations, neutophyls were predominant cell in the smears. In the chronic endometritis cases the most common cells seen in the smears were lymphocytes. The ratio of epitelial cells/leucocytes was determined as 2.7/1 in acute cases, 2.9/1 in chronic cases and 15/1 in healthy cases. As a result the endometrial cytology was found to a useful technique in the diagnosis of endometritis in cattle. 

Keywords

• Endometritis
• Endometrial sitoloji
• Tan yöntemleri

İneklerde Endometritisin Tanısında Klinik Muayene, Endometriyal Sitoloji, Biyopsi Ve Mikrobiyolojik Muayene Bulgularının Karşılaştırılması

Öz

Yapılan çalı mada ineklerde, endometritis tanısında endometriyal sitolojinin etkinli i, endometritislerin tanısında kullanılan di er bazı yöntemlerle kıyaslanarak de erlendirildi. Bu amaçla 13 akut, 17 kronik endometritisli ve 10 sa lıklı holstein inek kullanıldı. neklerin klinik muayeneleri, uterustan alınan örneklerin mikrobiyolojik ekimleri, uterus yıkantılarının sitolojik muayeneleri ile biyopsi örneklerinin histopatolojik muayeneleri yapıldı. Akut olgularda 13 olgunun 8inde vaginal akıntı görülmezken, 5 olguda vaginal akıntı gözlendi. Kronik endometritisli ve sa lıklı ineklerde herhangi bir vaginal akıntı tespit edilemedi. Akut, kronik ve sa lıklı ineklerdeki rektal palpasyon bulgularının birbirlerine benzerlikler gösterdi i belirlendi. Mikrobiyolojik ekimler sonrasında akut olgularda 10, kronik olgularda da 8 olguda olmak üzere A. pyogenes, E. coli, Streptococcus spp, Staphylococcus spp, K. Pneumonia ve Maya izole edilirken akut ve kronik toplam 12 olguda üreme olmadı. Histopatolojik muayenelerde akut endometritisli ineklerde lamina epitelyaliste dejenerasyon, deskuamasyon, nötrofil lökosit infiltrasyonu, lamina propriada ödem, hiperemi, ve nötrofil lökosit infiltrasyonu, bez lumenlerinde nötrofil lökosit infiltrasyonu, dökülmü bez epitelleri, uterus lumenlerinde ise nötrofil lökosit infiltrasyonu belirlendi. Kronik olgularda ise lamina propriada; mononükleer hücre infiltrasyonu, de i ik derecelerde ba doku artı ı, subepitelyal bölgede lenfoplazmositer hücre infiltrasyonları gözlemlendi. Sitolojik muayenelerde akut endometritislerde nötrofillerin fazla oldukları tespit edildi. Kronik endometritislerde dominant hücrelerin lenfositler oldu u belirlendi. Epitel/lökosit oranı akut endometritislerde ortalama 2.7/1, kronik endometritislerde 2.9/1 bulundu. Bu oran sa lıklı ineklerde 15 / 1 olarak belirlendi. Sonuç olaraki endometriyal sitolojinin ineklerde endometritis te hisinde saha artlarında kullanılabilece i kanısına varıldı

Anahtar Kelimeler

• Endometritis
• Endometrial sitoloji
• Tan yöntemleri

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