Deneysel Sıçan Sepsis Modelinde Bromelainin Oksidatif Stres ve Akciğer Doku Histopatolojisi Üzerine Etkileri

Abstract

Sepsis, inflamatuvar bir sendrom olup başta akciğeri hedef almaktadır. Bromelainin (BRO) anti-inflamatuvar özelliği ile bilinen enzim kompleksidir. Bu çalışmada Sprague dawley sıçanlarında çekal ligasyon ve perforasyon (CLP) ile indüklenen sepsise bağlı akciğer hasarında BRO koruyucu etkisi biyokimyasal ve histopatolojik yöntemlerle araştırıldı. Kırk adet Sprague dawley sıçan, kontrol, CLP, BRO, CLP+BRO 50 ve CLP+BRO 100 olmak üzere rastgele beş gruba ayrıldı. Çalışmada sıçanlara 50 ve 100 mg/kg BRO oral olarak (7 gün boyunca toplam 7 uygulama) verildikten sonra laparotomi ve CLP uygulanmıştır. Veriler, BRO'nun antioksidan özelliklerinden dolayı CLP ile indüklenen miyeloperoksidaz (MPO) ve lipid peroksidasyonu (MDA) azalttığını (p <0,001), glutatyon (GSH) seviyelerini, süperoksit dismutaz (SOD) ve glutatyon peroksidaz (GPx) aktivitelerini arttırdığını göstermiştir (p <0,001). Ayrıca CLP kaynaklı akciğer histolojik değişiklikler BRO takviyesi ile azalmıştır. Sonuçlar BRO tedavisin CLP kaynaklı akciğer toksisitesi üzerinde antioksidan, anti-inflamatuvar etkiye sahip olduğunu ve düzenleyici etkisi sayesinde akciğer doku mimarisinin korunmasına yardımcı olduğunu göstermiştir.

Keywords

• Akciğer
• Bromelain
• Sepsis
• İnflamasyon
• Oksidatif Stres

Effects of Bromelain on Oxidative Stress and Lung Tissue Histopathology in an Experimental Rat Sepsis Model

Abstract

Sepsis is an inflammatory syndrome that targets the lung tissue. Bromelain (BRO) is an enzyme complex known for its anti-inflammatory properties. In this study, the protective effect of BRO on lung injury due to sepsis induced by cecal ligation and puncture (CLP) in Sprague-Dawley rats was investigated by biochemical and histopathological methods. Forty Sprague dawley rats were randomly divided into five groups as control, CLP, BRO, CLP+BRO 50 and CLP+BRO 100. In the study, after oral administration of 50 and 100 mg/kg BRO (total of 7 applications for 7 days) to rats, laparotomy and CLP were performed. The data showed that due to its antioxidant properties, BRO decreased CLP-induced myeloperoxidase (MPO) and lipid peroxidation (MDA) (p <0,001), increased glutathione (GSH) levels, superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities (p <0,001). Additionally, CLP-induced lung histological changes were reduced with BRO supplementation. The results showed that BRO treatment has an antioxidant, anti-inflammatory effect on CLP-induced lung toxicity and helps preserve lung tissue architecture owing to its regulatory effect.

Keywords

• Lung
• Bromelain
• Sepsis
• Inflammation
• Oxidative Stress

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