Isolation of Some Bacterial Agents from Sheep and Goat Pneumonic Lung Samples and Determination of Antimicrobial Susceptibility

Abstract

Pneumonia cases in sheep and goats lead to significant economic losses around the world. The aim of this study is to isolate and identify Pasteurella multocida, Mannheimia haemolytica, Escherichia coli, Klebsiella pneumoniae, Histophilus somni and Trueperella pyogenes as well as to determine their antimicrobial susceptibilities in lung samples of sheep and goats that were delivered to the Siirt Municipal slaughterhouse and were detected to have pneumonic lesions in their lungs after slaughter. In the study, 429 lung samples were collected from 270 goats and 159 sheep slaughtered in the Siirt Municipal Slaughterhouse. P. multocida was identified in 8 (1.86%) out of the lung samples, M. haemolytica in 8 (1.86%), E. coli in 10 (2.33%), T. pyogenes in 2 (0.46%), and Klebsiella spp. in 1 (0.23%), while H. somni was not identified. According to antimicrobial susceptibility test results, it was determined that all P. multocida and M. haemolytica isolates were sensitive to cefpodoxime, ceftiofur, enrofloxacin, florfenicol, and spectinomycin; all T. pyogenes isolates were determined to be susceptible to danofloxacin, amoxicillin, clavulanic acid, oxytetracycline, erythromycin, ceftiofur, florfenicol, sulfamethoxazole/trimethoprim; and all E. coli isolates were susceptible to ertapenem, piperacillin/tazobactam, and cefoxitin. Consequently, it was determined that especially P. multocida, M. haemolytica and E. coli strains may cause pneumonia cases in sheep and goats raised in the Siirt region. Furthermore, the isolated strains were generally susceptible to antibiotics as a result of antimicrobial susceptibility tests.

Keywords

• Antimicrobial susceptibility
• Goat
• Pneumonia
• Sheep.

Koyun ve Keçi Pnömonik Akciğer Örneklerinden Bazı Bakteriyel Etkenlerin İzolasyonu ve Antimikrobiyal Duyarlılığının Belirlenmesi

Abstract

Koyun ve keçilerde meydana gelen pnömoni vakaları dünya çapında önemli ekonomik kayıplara neden olmaktadır. Bu araştırmada amaç; Siirt Belediye mezbahasına getirilen ve kesim sonrası akciğerlerinde pnömonik lezyonlar görülen koyun ve keçilerin akciğer örneklerindeki Pasteurella multocida, Mannheimia haemolytica, Esherichia coli, Klebsiella pneumoniae, Histophilus somni ve Trueperella pyogenes izole ve identifiye etmek ve antimikrobiyal duyarlılıklarını belirlemektir. Çalışma kapsamında Siirt Belediye mezbahasında kesimi yapılan 270 keçi ve 159 koyundan 429 akciğer örneği alındı. Alınan akciğer örneklerinin 8 (%1.86)’inden P. multocida, 8 (%1.86)’inden M. haemolytica, 10 (%2.33)’undan E. coli, 2 (%0.46)’sinden T. pyogenes, 1 (% 0.23)’inde de Klebsiella spp. identifiye edilirken, örneklerde H. somni belirlenmedi. Antimikrobiyal duyarlılık test sonuçlarına göre; P. multocida ve M. haemolytica izolatlarının tamamı sefpodoksim, seftiofur, enrofloksasin, florfenicol ve spektinomisine, T. pyogenes izolatlarının tamamı danofloksasin, amoksisilin klavulanik asit, oksitetrasiklin, eritromisin, seftiofur, florfenikol, sulfametoksazol/trimetoprime; E. coli izolatlarının tamamı ise; ertapenem, piperasilin/tazobaktam ve sefoksitine duyarlı olduğu belirlendi. Sonuç olarak bu çalışmada Siirt bölgesinde yetiştiriciliği yapılan koyun ve keçilerde meydana gelen pnömoni olgularına özellikle P. multocida, M. haemolytica ve E. coli suşlarının neden olabileceği belirlendi. Ayrıca izole edilen suşların yapılan antimikrobiyal duyarlılık testleri sonucunda antibiyotiklere genel olarak duyarlı olduğu sonucuna varıldı.

Keywords

• Antimikrobiyal duyarlılık
• Keçi
• Koyun.
• Pnömoni

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