Isolation of Some Bacterial Agents from Sheep and Goat Pneumonic Lung Samples and Determination of Antimicrobial Susceptibility

Öz

Pneumonia cases in sheep and goats lead to significant economic losses around the world. The aim of this study is to isolate and identify Pasteurella multocida, Mannheimia haemolytica, Escherichia coli, Klebsiella pneumoniae, Histophilus somni and Trueperella pyogenes as well as to determine their antimicrobial susceptibilities in lung samples of sheep and goats that were delivered to the Siirt Municipal slaughterhouse and were detected to have pneumonic lesions in their lungs after slaughter. In the study, 429 lung samples were collected from 270 goats and 159 sheep slaughtered in the Siirt Municipal Slaughterhouse. P. multocida was identified in 8 (1.86%) out of the lung samples, M. haemolytica in 8 (1.86%), E. coli in 10 (2.33%), T. pyogenes in 2 (0.46%), and Klebsiella spp. in 1 (0.23%), while H. somni was not identified. According to antimicrobial susceptibility test results, it was determined that all P. multocida and M. haemolytica isolates were sensitive to cefpodoxime, ceftiofur, enrofloxacin, florfenicol, and spectinomycin; all T. pyogenes isolates were determined to be susceptible to danofloxacin, amoxicillin, clavulanic acid, oxytetracycline, erythromycin, ceftiofur, florfenicol, sulfamethoxazole/trimethoprim; and all E. coli isolates were susceptible to ertapenem, piperacillin/tazobactam, and cefoxitin. Consequently, it was determined that especially P. multocida, M. haemolytica and E. coli strains may cause pneumonia cases in sheep and goats raised in the Siirt region. Furthermore, the isolated strains were generally susceptible to antibiotics as a result of antimicrobial susceptibility tests.

Anahtar Kelimeler

• Antimicrobial susceptibility
• Goat
• Pneumonia
• Sheep.

Koyun ve Keçi Pnömonik Akciğer Örneklerinden Bazı Bakteriyel Etkenlerin İzolasyonu ve Antimikrobiyal Duyarlılığının Belirlenmesi

Öz

Koyun ve keçilerde meydana gelen pnömoni vakaları dünya çapında önemli ekonomik kayıplara neden olmaktadır. Bu araştırmada amaç; Siirt Belediye mezbahasına getirilen ve kesim sonrası akciğerlerinde pnömonik lezyonlar görülen koyun ve keçilerin akciğer örneklerindeki Pasteurella multocida, Mannheimia haemolytica, Esherichia coli, Klebsiella pneumoniae, Histophilus somni ve Trueperella pyogenes izole ve identifiye etmek ve antimikrobiyal duyarlılıklarını belirlemektir. Çalışma kapsamında Siirt Belediye mezbahasında kesimi yapılan 270 keçi ve 159 koyundan 429 akciğer örneği alındı. Alınan akciğer örneklerinin 8 (%1.86)’inden P. multocida, 8 (%1.86)’inden M. haemolytica, 10 (%2.33)’undan E. coli, 2 (%0.46)’sinden T. pyogenes, 1 (% 0.23)’inde de Klebsiella spp. identifiye edilirken, örneklerde H. somni belirlenmedi. Antimikrobiyal duyarlılık test sonuçlarına göre; P. multocida ve M. haemolytica izolatlarının tamamı sefpodoksim, seftiofur, enrofloksasin, florfenicol ve spektinomisine, T. pyogenes izolatlarının tamamı danofloksasin, amoksisilin klavulanik asit, oksitetrasiklin, eritromisin, seftiofur, florfenikol, sulfametoksazol/trimetoprime; E. coli izolatlarının tamamı ise; ertapenem, piperasilin/tazobaktam ve sefoksitine duyarlı olduğu belirlendi. Sonuç olarak bu çalışmada Siirt bölgesinde yetiştiriciliği yapılan koyun ve keçilerde meydana gelen pnömoni olgularına özellikle P. multocida, M. haemolytica ve E. coli suşlarının neden olabileceği belirlendi. Ayrıca izole edilen suşların yapılan antimikrobiyal duyarlılık testleri sonucunda antibiyotiklere genel olarak duyarlı olduğu sonucuna varıldı.

Anahtar Kelimeler

• Antimikrobiyal duyarlılık
• Keçi
• Koyun.
• Pnömoni

Kaynakça

1. Abera, D., & Mossie, T. (2023). A review on pneumonic pasteurellosis in small ruminants. Journal of Applied Animal Research, 51(1), 1-10. https://doi.org/10.1080/09712119.2022.2146123
2. Adam, M., Akeem, A. O., Barka, S. A., Olu, S. S. V., Abiodun, A. A., & David, O. F. S. (2023). Pathological and molecular investigation of some bacterial in the lungs and livers of red sokoto goats slaughtered at Ilorin, Kwara State, Nigeria. Media Kedokteran Hewan, 34(2), 87-101. doi: 10.20473/mkh.v34i2.2023.87-101
3. Akane, A. E., Alemu, G., Tesfaye, K., Ali, D. A., Abayneh, T., Kenubih, A., Ejo, M., Abat, A. S., Admassu, B., & Ibrahim, S. M. (2022). Isolation and molecular detection of Pasteurellosis from pneumonic sheep in selected areas of Amhara region, Ethiopia: an implication for designing effective ovine Pasteurellosis vaccine. Veterinary Medicine: Research and Reports, 13,75-83.
4. Alali, W. Q., AlFouzan, W., & Dhar, R. (2021). Prevalence of antimicrobial resistance in Gram-negative clinical isolates from a major secondary hospital in Kuwait: a retrospective descriptive study. GERMS, 11(4), 498-511. doi: 10.18683/germs.2021.1285
5. Angen, Ø., Ahrens, P., Kuhnert, P., Christensen, H., & Mutters, R. (2003). Proposal of Histophilus somni gen. nov., sp. nov. for the three species incertae sedis ‘Haemophilus somnus’,‘Haemophilus agni and ‘Histophilus ovis’. International Journal of Systematic and Evolutionary Microbiology, 53(5), 1449-1456. https://doi.org/10.1099/ijs.0.02637-0
6. Babu, R. S. D., Balasubramaniam, G. A., Murthy, T., Sivaseelan, S., & Udhayavel, S. (2019). Isolation and identification of bacterial species from small ruminants affected with pneumonia and their antibiogram pattern. Journal of Entomology and Zoology Studies, 7(1), 621-3.
7. Bakır, G., & Mikail, N. (2019). Siirt ilindeki küçükbaş hayvancılık işletmelerinin yapısal durumu. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 50, 66-74. doi: 10.17097/ataunizfd.411087
8. Baykan, Z. K., Tabak, M. H., Kılıç, A., Gün, H., & Mete, A. (2023). Investigation and epidemiology of agents isolated from the lungs of cattle, sheep, and goats with pneumonia in the Marmara Region using bacteriological methods. Kocatepe Veterinary Journal, 16(2), 209-218. https://doi.org/10.30607/kvj.1208946

9. Besser, T. E., Highland, M. A., Baker, K., Cassirer, E. F., Anderson, N. J., Ramsey, J. M., Mansfield, K., Bruning, D. L., Wolff, P., Smith, J. B., & Jenks, J. A. (2012). Causes of pneumonia epizootics among bighorn sheep, western United States, 2008–2010. Emerging Infectious Diseases, 18(3), 406. doi: 10.3201/eid1803.111554
10. Chakraborty, S., Kumar, A., Tiwari, R., Rahal, A., Malik, Y., Dhama, K., Pal, A., Prasad, M. (2014). Advances in diagnosis of respiratory diseases of small ruminants. Veterinary Medicine International, 2014(1), 508304. https://doi.org/10.1155/2014/508304
11. Chong, Y., Ito, Y., & Kamimura, T. (2011). Genetic evolution and clinical impact in extendedspectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae. Infection, Genetics and Evolution, 11, 1499-1504. http://dx.doi.org/10.1016/j.meegid.2011.06.001
12. Clinical and Laboratory Standards Institute (CLSI) (2002). Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Approved Standard, CLSI Document M31-A2, second ed 2002. Wayne, PA.
13. Clinical and Laboratory Standards Institute (CLSI) (2003). performance standards for antimicrobial disk susceptibility tests. Approved Standard CLSI Document M2-A8, 2003. Wayne, PA.
14. Clinical and Laboratory Standards Institute (CLSI) (2018). Performance standards for antimicrobial disk dilution susceptibility tests for bacteria isolated from animals; approved standard. 4th ed. CLSI document VET08 38, 20-126.
15. Clinical and Laboratory Standards Institute (CLSI) (2023). Performance standards for antimicrobial disk dilution susceptibility tests for bacteria isolated from animals; approved standard. 6th ed. CLSI document VET01S 43, 26-146.
16. Dabo, S. M., Taylor, J. D., & Confer, A. W. (2007). Pasteurella multocida and bovine respiratory disease. Animal Health Research Reviews, 8(2), 129-150. doi: https://doi.org/10.1017/S1466252307001399
17. El-Mashad, A. B. I., Moustafa, S. A., Amin, A., & Samy, E. M. (2020). Pathological studies on lung affections in sheep and goat at Kalubia Governorate. Benha Veterinary Medical Journal, 38(1), 17-23. doi: 10.21608/bvmj.2020.25089.1178
18. European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2023). Clinical breakpoint tables v. 13.1, valid from 2023-06-29. 13-86.
19. Ferdausi, T., Haider, M. G., Alam, K. J., Baki, M. A., & Hossain, M. M. (2008). Caprine lung diseases and causal bacteria. Bangladesh Veterinarian, 25(1), 9-16. https://doi.org/10.3329/bvet.v25i1.4613
20. Fernandez, S., Galapero, J., Gomez,L., Perez, C. J., Cid, D., Martín, M. C., Alonso, J.M., & Rey, J. (2016). Mannheimia haemolytica and Bibersteinia trehalosi serotypes ısolated from merino breed lambs in Extremadura (Southwestern Spain). Indian Journal of Microbiology, 56, 513-515.
21. Gulaydın, O., & Gurturk, K. (2018). Identification of Pasteurella multocida strains isolated from respiratory tract of healthy and diseased cattle and determination of capsular types by PCR. Van Veterinary Journal, 29(3), 143-146.
22. Gulaydin, O., Gurturk, K., Ekin, I. H., & Ozturk, C. (2021). Determination of MIC values of various antimicrobial agents and presence of resistance genes in Pasteurella multocida strains isolated from bovine. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(2). doi: 10.9775/kvfd.2020.25093
23. Hanthorn, C. J., Dewell, R. D., Cooper, V. L., Frana, T. S., Plummer, P. J., Wang, C., & Dewell, G. A. (2014). Randomized clinical trial to evaluate the pathogenicity of Bibersteinia trehalosi in respiratory disease among calves. BMC Veterinary Research, 10, 1-8.
24. Hawari, A. D., Hassawi, D. S., & Sweiss, M. (2008). Isolation and identification of Mannheimia haemolytica and Pasteurella multocida in sheep and goats using biochemical tests and random amplified polymorphic DNA (RAPD) analysis. Journal of Biological Sciences, 8(7), 1251-1254.
25. Headley, S. A., Okano, W., Balbo, L. C., Marcasso, R. A., Oliveira, T. E., Alfieri, A. F., Filho, L. C. N., Michelazzo, M. Z., Rodrigues, S. C., Baptista, A. L., Saut, J. P. E., & Alfieri, A. A. (2018). Molecular survey of infectious agents associated with bovine respiratory disease in a beef cattle feedlot in southern Brazil. Journal of Veterinary Diagnostic Investigation, 30(2), 249-251. https://doi.org/10.1177/1040638717739
26. Humphrey, J. D., & Stephens, L. R. (1983). Haemophilus somnus: a review. Veterinary Bulletin, 53, 987-1004. Jarikre, T. A., Alao, O. S., & Emikpe, B. O. (2018). Cultural and immunohistochemical evaluation of bacterial agents in caprine pneumonia in Nigeria. Comparative Clinical Pathology, 27, 1051-1055.
27. Kılıç, D. (2004). Hayvan beslenmesinde antibiyotik kullanımı ve direnç. Flora, 9(1), 29-36.
28. Krishnamurthy, V., Vijaykumar, G. S., Sudeepa Kumar, M., Prashanth, H. V., Prakash, R., & Nagaraj, E. R. (2013). Phenotypic and genotypic methods for detection of extended spectrum β lactamase producing Escherichia coli and Klebsiella pneumoniae isolated from ventilator associated pneumonia. Journal of Clinical and Diagnostic Research, 7(9), 1975-1978. doi: 10.7860/JCDR/2013/6544.3376
29. Mahmoud, M. A., Osman, W. A., Goda, A. S., & El Naggar, A. L. (2005). Prevalence of some respiratory diseases among sheep and goats in Shalateen, Halaieb and Abu-Ramad Areas. Journal of Veterinary Medical Research, 15(2), 196-202. doi: 10.21608/jvmr.2005.77976
30. Mahrous, S. H., El-Balkemy, F. A., Abo-Zeid, N. Z., El-Mekkawy, M. F., El Damaty, H. M., & Elsohaby, I. (2023). Antibacterial and anti-biofilm activities of cinnamon oil against multidrug-resistant Klebsiella pneumoniae isolated from pneumonic sheep and goats. Pathogens, 12(9), 1138. https://doi.org/10.3390/pathogens12091138
31. Marru, H. D., Anijajo, T. T., & Hassen, A. A. (2013). A study on Ovine pneumonic pasteurellosis: Isolation and identification of Pasteurellae and their antibiogram susceptibility pattern in Haramaya District, Eastern Hararghe, Ethiopia. BMC Veterinary Research, 9, 1-8.
32. Nejiban, Z. S., & Al-Amery, M. A. (2018). Clinical and diagnostic study of sheep Pneumonic pasteurellosis in Basrah, Iraq. Al-Qadisiyah Journal of Veterinary Medicine Sciences, 17(1), 1-5.
33. Oruc, E. (2006). The pathologic and bacteriologic comparison of pneumonia in lambs. Turkish Journal of Veterinary & Animal Sciences, 30(6), 593-599.
34. Özavcı, V., Nuhay, Ç., & Özden, M. (2022). Ege bölgesinde koyun ve kuzu pnömonisi olgularında Pasteurella Multocida ve Mannheimia Haemolytica izolasyonu ve antibiyotik dirençlerinin tespiti. Etlik Veteriner Mikrobiyoloji Dergisi,, 33(2), 59-63. doi: https://doi.org/10.35864/evmd.1133898 33, 59-63
35. Özbey, G., & Muz, A. (2004). Isolation of aerobic bacteria from the lungs of sheep and goats with pneumonia and detection of isolated Pasteurella multocida and Mannheimia haemolytica by polymerase chain reaction. Turkish Journal of Veterinary & Animal Sciences, 28, 209-216.
36. Pérez-Romero, N., Aguilar-Romero, F., Arellano-Reynoso, B., Díaz-Aparicio, E., & Hernández-Castro, R. (2011). Isolation of Histophilus somni from the nasal exudates of a clinically healthy adult goat. Tropical animal health and production, 43, 901-903.
37. Quinn, P. J., Markey, B. K., Leonard, F. C., Hartigan, P., Fanning, S., & Fitzpatrick, E. (2011). Veterinary microbiology and microbial disease. John Wiley & Sons. 263-314.
38. Rahman, M. H., Ahmed, E., Haque, M. N., Hassan, M. Z., & Ali, M. Z. (2022). Major respiratory diseases of goat and their epidemiology, prevention and control. Bangladesh Journal of Livestock Research, 1-20. https://doi.org/10.3329/bjlr.v29i1.72031
39. Rashid, M. M., Ferdoush, M. J., Dipti, M., Roy, P., Rahman, M. M., Hossain, M. I., & Hossain, M. M. (2013). Bacteriological and pathological investigation of goat lungs in Mymensingh and determination of antibiotic sensitivity. Bangladesh Journal of Veterinary Medicine, 11(2).
40. Semerci, A., & Çelik, A. D. (2016). General overview of ovine breeding in Turkey. Journal of Agricultural Faculty of Mustafa Kemal University, 21(2), 182-196.
41. Sikrodia, R., Chhabra, D., Shukla, S., Mehta, H., Audarya, S. D., Gangil, R., Jogi, J., & Sharda, R. (2022). Isolation, identification and antimicrobial resistance in Klebsiella spp. isolated from diarrheic cattle calves in Mhow. The Pharma Innovation Journal, 11(9), 1391-1395.
42. Singh, F., Sonawane, G. G., Kumar, J., Dixit, S. K., Meena, R. K., & Tripathi, B. N. (2019). Antimicrobial resistance and phenotypic and molecular detection of extended-spectrum ß-lactamases among extraintestinal Escherichia coli isolated from pneumonic and septicemic sheep and goats in Rajasthan, India. Turkish Journal of Veterinary & Animal Sciences, 43(6), 754-760. doi: 10.3906/vet-1905-1
43. Tel, O. Y., & Keskin, O. (2010). Isolation and antibiotic susceptibility of Pasteurella multocida and Mannheimia haemolytica from sheep lungs. YYU Veterinary Faculty Journal, 21, 31-34.
44. Tesfaye, B., Sisay Tessema, T., & Tefera, G. (2013). Diversity of bacterial species in the nasal cavity of sheep in the highlands of Ethiopia and first report of Histophilus somni in the country. Tropical Animal Health and Production, 45, 1243-1249.
45. Thakur, S., Verma, S., Dhar, P., & Sharma, M. (2019). Molecular detection of bacterial pathogens directly from the nasal swabs and lung tissue of sheep and goats. Indian Journal of Veterinary Sciences and Biotechnology, 15(2), 22-25.
46. Townsend, K. M., Frost, A. J., Lee, C. W., Papadimitriou, J. M., & Dawkins, H. J. (1998). Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates. Journal of cCinical Microbiology, 36(4), 1096-1100. https://doi.org/10.1128/jcm.36.4.1096-1100.1998
47. Türkiye İstatistik Kurumu. (TÜİK) (2023). Hayvansal Üretim Istatistikleri, Aralık.
48. Turton, J. F., Perry, C., Elgohari, S., & Hampton, C. V. (2010). PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets. Journal of Medical Microbiology, 59(5), 541-547. https://doi.org/10.1099/jmm.0.015198-0
49. Ülbegi-Mohyla, H., Hijazin, M., Alber, J., Lämmler, C., Hassan, A. A., Abdulmawjood, A., Prenger-Berninghoff, E., Weiss, R., & Zschöck, M. (2010). Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties. Journal of Veterinary Science, 11(3), 265-267. doi: https://doi.org/10.4142/jvs.2010.11.3.265
50. Ülgen, M., Sönmez, G., & Aydın, F. (1997). Microbiological and histopathological investigations on lamb pneumonia. Etlik Veteriner Mikrobiyoloji Dergisi, 9(2), 55-70.
51. Valadan, M., Jabbari, A. R., Niroumand, M., Tahamtan, Y., & Bani Hashemi, S. R. (2014). Isolation and identification of Pasteurella multocida from sheep & goat in Iran. Archives of Razi Institute, 69(1), 47-55. doi: 10.7508/ari.2014.01.007
52. Wang, X-R., Chen, C-J., Yu, K., Ning, J., Shu-Chang, A., & Zhan-Cheng, G. (2012). Prevalence and characterization of plasmid-mediated bla ESBL with their genetic environment in Escherichia coli and Klebsiella pneumoniae in patients with pneumonia. Chinese Medical Journal, 125, 894-900. doi: 10.3760/cma.j.issn.0366-6999.2012.05.029
53. Ward, A. C., Weiser, G. C., Anderson, B. C., Cummings, P. J., Arnold, K. F., & Corbeil, L. B. (2006). Haemophilus somnus (Histophilus somni) in bighorn sheep. Canadian Journal of Veterinary Research, 70(1), 34.