Morphological and Histological Investigation of the Effects of Garlic Extract on the Intestine of Goldfish (Carassius auratus auratus)

Abstract

Garlic (Allium sativum) has been widely recognized for its therapeutic properties and potential use as a feed additive in aquaculture. This study aimed to investigate the morphological and histological effects of garlic extract on the intestinal health and growth performance of goldfish (Carassius auratus auratus). Goldfish were fed diets supplemented with 5 and 10 mg/g of garlic extract for three months. Results indicated significant improvements in both weight gain and intestinal villus length, particularly in the 10 mg/g group, compared to the control group. The control group showed a decline of 21% in the overall metric between the two sampling periods, with intermediate percentage increases during the individual sampling intervals. In contrast, the groups receiving garlic supplementation demonstrated significant increases. The 5 mg/g garlic group exhibited a 37% total increase, while the 10 mg/g garlic group showed the highest overall increase of 53%. These findings suggest that garlic extract can enhance digestive efficiency and overall growth in goldfish, presenting a promising natural alternative for growth promoters in ornamental fish species. Further research is recommended to explore its long-term effects and efficacy across different fish species.

Keywords

• Allium sativum
• Garlic Extract
• Intestinal Histology

Project Number

TUBITAK (1919B012210176)

Ethical Statement

The experimental procedures were conducted in accordance with ethical guidelines. Approval was obtained from the Local Ethics Committee (decision number: 2022-17/0).

Sarımsak Ekstraktının Japon Balığının (Carassius auratus auratus) Bağırsağı Üzerindeki Etkilerinin Morfolojik ve Histolojik Olarak Araştırılması

Abstract

Sarımsak (Allium sativum), terapötik özellikleri ve su ürünleri yetiştiriciliğinde yem katkı maddesi olarak potansiyel kullanımıyla geniş çapta tanınmıştır. Bu çalışma, sarımsak ekstraktının Japon balığının (Carassius auratus auratus) bağırsak sağlığı ve büyüme performansı üzerindeki morfolojik ve histolojik etkilerini araştırmayı amaçlamıştır. Japon balıkları, üç ay boyunca 5 ve 10 mg/g sarımsak ekstraktı ile zenginleştirilmiş diyetlerle beslenmiştir. Sonuçlar, özellikle 10 mg/g grubunda, kontrol grubuna kıyasla hem kilo alımında hem de bağırsak villus uzunluğunda anlamlı iyileşmeler olduğunu göstermiştir. Kontrol grubunda, iki örnekleme dönemi arasında toplamda %21’lik bir azalma gözlenirken, bireysel örnekleme aralıklarında orta derecede artışlar kaydedilmiştir. Buna karşılık, sarımsak takviyesi alan gruplar önemli artışlar sergilemiştir. 5 mg/g sarımsak grubunda toplam %37’lik bir artış gözlemlenirken, 10 mg/g sarımsak grubunda %53 ile en yüksek toplam artış tespit edilmiştir. Bu bulgular, sarımsak ekstraktının Japon balıklarında sindirim verimliliğini ve genel büyümeyi artırabileceğini, süs balığı türleri için umut vadeden doğal bir büyüme teşvik edici alternatif sunduğunu göstermektedir. Uzun vadeli etkilerini ve farklı balık türlerindeki etkinliğini araştırmak üzere daha fazla çalışmaya ihtiyaç duyulmaktadır.

Keywords

• Allium sativum
• Bağırsak Histolojisi
• Sarımsak Ekstraktı

Project Number

TUBITAK (1919B012210176)

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