Assessment of DNA Damage and Oxidant/Antioxidant Balance After Acute Exposure to Glyphosate Isopropylamine in Rats

Abstract

In this study, the effects of oral administration of glyphosate isopropylamine (GI) salt at different doses (1/10 and 1/2 of LD50) on DNA damage, oxidant/antioxidant balance and some biochemical parameters were investigated to evaluate the acute toxicity of glyphosate isopropylamine salt. The results showed that 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels were significantly increased, malondialdehyde (MDA) levels were increased in all tissues except blood and heart and glutathione (GSH) levels were decreased in rats in the high dose group. In addition, significant increases in catalase (CAT) and superoxide dismutase (SOD) enzyme activities were detected. Biochemical analyses demonstrated that acute exposure to herbicides led to a significant elevation in serum alkaline phosphatase (ALP), albumin and urea levels, but no significant changes in AST, ALT, GGT, total protein (Tp), creatinine and CK-MB levels. These findings suggest that exposure to high doses of glyphosate may cause toxic effects by triggering oxidative stress and DNA damage.

Keywords

• Acute exposure
• Glyphosate isopropylamine
• Rats

Sıçanlarda Glifosat İzopropilamin Akut Maruziyeti Sonrası DNA hasarı ve Oksidan/Antioksidan Denge Değerlendirmesi

Abstract

Bu çalışmada, glifosat izopropilamin (GI) tuzunun akut toksisitesini değerlendirmek amacıyla sıçanlara farklı dozlarda (LD50’nin 1/10 ve 1/2’si) oral olarak uygulanmasının, DNA hasarı, oksidan/antioksidan denge ve bazı biyokimyasal parametreler üzerindeki etkileri incelenmiştir. Sonuçlar, yüksek doz grubundaki sıçanlarda 8-hidroksi-2’-deoksiguanozin (8-OHdG) seviyelerinin belirgin şekilde arttığını, malondialdehit (MDA) düzeylerinin kan ve kalp dışındaki tüm dokularda yükseldiğini ve glutatyon (GSH) seviyelerinin ise azaldığını göstermiştir. Ayrıca, katalaz (CAT) ve süperoksit dismutaz (SOD) enzim aktivitelerinde anlamlı artışlar tespit edilmiştir. Biyokimyasal analizler, herbisite akut maruziyetin serum alkalen fosfataz (ALP), albümin ve üre seviyelerinde artışa yol açtığını ancak AST, ALT, GGT, total protein (Tp), kreatinin ve CK-MB seviyelerinde belirgin bir değişiklik oluşturmadığını ortaya koymuştur. Bu bulgular, yüksek dozda glifosata maruziyetin oksidatif stres ve DNA hasarını tetikleyerek toksik etkilere neden olabileceğini göstermektedir.

Keywords

• Akut maruziyet
• Glifosat izopropilamin
• Sıçan

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