Sıçanlarda Bekletilmiş, Distile ve Deiyonize Kaplıca Suyu Tüketilmesinin Oksidan Antioksidan Denge ve Bazı Biyokimyasal Parametreler Üzerine Etkisinin Belirlenmesi

Abstract

Bu çalışmada, Afyonkarahisar ilinin Gazlıgöl bölgesinden alınan kaplıca suyu ve bu suyun bekletilerek, distile ve deiyonize edilerek tüketilmesinin oksidan-antioksidan denge ve bazı biyokimyasal parametreler ile karaciğer ve böbrek dokusuna etkilerinin belirlenmesi amaçlanmıştır. Çalışmada her grupta 6 sıçan yer almıştır. 30 gün boyunca, deney grubundaki sıçanlara taze, bekletilmiş, deiyonize ve distile edilmiş kaplıca suyu, kontrol grubundakilere ise normal su verilmiştir. Deneme sonunda sıçanlardan kan numuneleri ile karaciğer, böbrek ve kalp doku örnekleri alınmıştır.Hemoglobin değeri bekletilmiş kaplıca suyu tüketen sıçanlarda taze ve deiyonize tüketen sıçanlara göre önemli oranda düşük olduğu gözlenmiştir(p<0,05). Distile edilmiş kaplıca suyu tüketen sıçanlarda Çinko değerinde diğer gruplara göre anlamlı (p<0,05) bir düşüş oluşmuştur. Karaciğer ve böbrek dokusu MDA, GSH ve SOD düzeyleri bakımından gruplar arasında anlamlı bir değişim gözlenmemiştir. Buna karşın, kalp dokusunda MDA düzeylerinin bekletilmiş ve deiyonize kaplıca suyu tüketen sıçanlarda diğer gruptakilere göre daha yüksek olduğu (p<0,01), GSH ile SOD düzeylerinin ise değişmediği saptanmıştır. Karaciğer ve böbrek dokusu hispatolojik incelemesinde birtakım patolojik değişiklikler tespit edilmiştir. Sonuç olarak çalışmada kullanılan kaplıca suyunun organizmada olumlu bir etki yaratmadığı kanısına varılmıştır.

Keywords

• Biyokimyasal Parametre,Oksidan Antioksidan Denge,Kaplıca Suyu

Determination of Effects on Oxidant Antioxidant Balance and Some Biochemical Parameters Consumption of Bottled, Distilled and Deionized Thermal Water in Rats

Abstract

The objective of this research is to determine the effects of the consumption of the bottled, distilled and deionized versions of thermal water taken from the Gazlıgöl district in the city of Afyonkarahisar on the oxidant – antioxidant balance and on certain biochemical parameters, along with possible effects on liver and kidney tissue. The research involved 6 rats in each group. The experiment group was given fresh, bottled, deionized and distilled thermal water for 30 days whereas the control group was given normal water. At the end of the experiment, rats’ blood samples as well as liver, kidney and heart tissue samples were taken. The research determined that the hemoglobin level in rats that consumed bottled thermal water is considerably lower than those that consumed fresh and deionized water (p<0,05). The zinc level was meaningfully lower in rats that consumed distilled water than those in the other groups (p<0,05). There was no meaningful difference among the groups in terms of the liver and kidney tissues’ MDA, GSH and SOD levels. Nevertheless, it was observed that the heart tissues’ MDA level was higher in rats that consumed bottled and deionized water than those in other groups (p<0,01) whereas the GSH and SOD levels did not change. The histopathological examination of liver and kidney tissue showed certain pathological changes.The conclusion of the research is that thermal water does not have a positive effect on the organism.

Keywords

• Biochemical Parameters,Oxidant Antioxidant Balance,Thermal Water

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