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Afyonkarahisar İlinde Satışa Sunulan Tost Peynirlerinin Bazı Fizikokimyasal ve Mikrobiyolojik Kalite Kriterlerinin İncelenmesi

Year 2019, , 235 - 241, 30.09.2019
https://doi.org/10.30607/kvj.557787

Abstract

Bu çalışmada
Afyonkarahisar il merkezinde marketlerde satışa sunulan tost peynirlerinin
fizikokimyasal ve mikrobiyolojik kaliteleri incelemiştir. Örneklerin asitlik
(pH) ve su aktivitesi (aw) değerleri ortalama olarak, sırasıyla 4.89
ve 0.932 olarak belirlenmiştir. Yapılan mikrobiyolojik analizler sonucunda;
toplam aerobik bakteri, toplam aerobik psikrofilik bakteri, Maya Küf, toplam
koliform grubu bakteri, laktik asit bakteri sayıları, Lactococcus/Streptococcus spp., Pseudomonas
spp., Staphylococcus aureus, Bacillus
cereus
ortalama sayıları ise sırasıyla; 5.81, 3.30, 4.14, 2.89, 4.69, 4.93,
4.74, 2.42 ve <2.00 log kob/g olarak tespit edilmiştir. Ayrıca örneklerin
%24’ünde Escherichia coli, %8’inde Clostridium perfringens, %18’inde Salmonella spp. cinsi, %12’sinde Listeria spp.cinsi bakteri varlığı
belirlemesine karşın örneklerin hiçbirisinde Brucella spp. cinsi bakteri gelişimi tespit edilmemiştir.

References

  • Akin N. Basic Cheese Science-1: Basic Topics. Damla Ofset Publications, Konya, Turkey. 2010. (In Turkish).
  • Anonymous. Turkish Standards Institute. TS 6235 EN ISO 6887-1. Microbiology of Food and Animal Feed General Rules of The Initial Suspension and Decimal Dilutions of Test Samples Preparation. 2001.
  • Anonymous. Turkish Food Codex. Cheese Communiqué, Communiqué No: 2015/6. 2015.
  • Aran N. A microbial study of Kashar cheese. Milchwissenschaft. 1998; 53: 565–567.
  • Aytac SA, Taban BM. Food-Borne Intoxications, In: Food Microbiology, Ed; Erkmen O. 5th Ed., Efil Publishing House, Ankara, Turkey. 2017; pp.155-156. (In Turkish).
  • Cetin B, Karasu S, Atik A, Durak MZ. Investigation of microbiological quality of some dairy products in Kırklareli: Detection of Salmonella spp. and Listeria monoctyogenes by Real Time PCR. Journal of Tekirdag Agricultural Faculty. 2015; 12(1): 74-80.
  • Cetinkaya A. Our Traditional Chesses. Abp Press, İstanbul, Turkey. 2008. (In Turkish).
  • Cetinkaya F, Soyutemiz GE. Microbiological and chemical changes throughout the manufacture and ripening of Kashar: a traditional Turkish cheese. Turk. J. Vet. Anim. Sci. 2006; 30: 397-404.
  • Corroler D, Mangin I, Desmasure N, Gueguen M. An ecological study of lactococci isolated from raw milk in the Camembert cheese registered designation of origin area. Appl. Environ. Microbiol. 1998; 64(12): 4729–4735.
  • Dogan HB, Tukel Cİ. Total (aerobic mesophilic) bacteria. Food Microbiology and applications, 2nd Ed., Sim Printing Co, Ankara, Turkey. 2000; pp. 323-328.(In Turkish).
  • Flowers RS, D’aust JY, Andrews WH, Bailey JS. Salmonella In: Compendium of the Methods for the Microbiological Examinations of Foods. Eds; Vanderzant C, Spilttstoesser DF. American Public Health Association. 1992; pp. 371-422.
  • Greenwood MH, Coetzee EF, Ford BM, Gill P, Hooper WL, Matthews SCV, Patrick S. The microbiology of selected retail food products with an evolation of vialable counting methods. Journal of Hygiene. 1984; 92: 67-77.
  • Guinee TP, Caric M, Kalab M. Pasteurized Processed Cheese and Substitute/Imitation Cheese Products, In: Cheese: Chemistry, Physics and Microbiology, Major Cheese Groups. Fox PF. Elsevier Applied Science, London, U.K. 2004; pp. 349-394.
  • Gulmez M, Oral N, Guven A, Baz E, Sezer C, Duman B. Some microbiological and chemical properties of kashar cheese sold in Kars. Journal of the Faculty of Veterinary Medicine, Kafkas University. 2004; 10 (2): 183-188.
  • Halkman K, Sagdas OE. Food Microbiology Applications. Prosigma Printing and Promotional Services, Ankara, Turkey. 2011.(in Turkish).
  • ISO. International Standard Organization. 4832, General guidance fort the enumeration of coliforms colony count technique. Geneva, Switzerland. 1991.
  • ISO. International Standard Organization. 6888-1, Horizontal method for the enumeration of coagulase- positive staphylococci technique using Baird Parker agar medium. Geneva, Switzerland. 1999.
  • ISO. International Standard Organization. 16649-1:2001, Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli , Part 1: Colony count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide. Geneva, Switzerland. 2001.
  • ISO. International Standard Organization. 7932:2004, Microbiology of food and animal feeding stuffs, Horizontal method for the enumeration of presumptive Bacillus cereus, Colony count technique at 30 degrees C. Geneva, Switzerland. 2004a.
  • ISO. International Standard Organization. 7937:2004, Microbiology of food and animal feeding stuffs, Horizontal method for the enumeration of Clostridium perfringens, Colony count technique. Geneva, Switzerland. 2004b.
  • ISO. International Standard Organization. 21527-1:2008, Microbiology of food and animal feeding stuffs, Horizontal method for the enumeration of yeasts and molds, Part 1: Colony count technique in products with water activity greater than 0,95. Geneva, Switzerland. 2008.
  • ISO. International Standard Organization. 13720:2010, specifies a method for the enumeration of presumptive Pseudomonas spp. present in meat and meat products, including poultry. Geneva, Switzerland. 2010.
  • ISO. International Standard Organization. 4833-1:2013, Microbiology of the food chain, Horizontal method for the enumeration of microorganisms, Part 1: Colony count at 30 degrees C by the pour plate technique. Geneva, Switzerland. 2013a.
  • ISO. International Standard Organization. 4833-2:2013, Horizontal method for the enumeration of microorganisms, Part 2: Colony count at 30 degrees C by the surface plating technique. Geneva, Switzerland. 2013b.
  • ISO. International Standard Organization. 16649-3:2015, Microbiology of the food chain, Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli, Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide. Geneva, Switzerland. 2015.
  • ISO. International Standard Organization. 6579-1:2017, Horizontal method for the detection, enumeration and serotyping of Salmonella. Geneva, Switzerland. 2017a.
  • ISO. International Standard Organization. 11290-1:2017, Microbiology of the food chain, Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. Part 1: Detection method. Geneva, Switzerland. 2017b.
  • ISO. International Standard Organization. 11290-2:2017, Microbiology of the food chain, Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. , Part 2: Enumeration method. Geneva, Switzerland. 2017c.
  • Kara R. A traditional cheese: characterization of Afyon Tulum cheese and investigation of reproduction and viability of empirically inoculated Brucella abortus and Brucella melitensis strains. PhD thesis, Afyon Kocatepe University Health Sciences Institute, Afyonkarahisar, 2011.
  • Karagozlu N. Food Borne Toxi infections. In: Food Microbiology, Ed; Erkmen O. 5th Ed., Efil Publishing House, Ankara, Turkey. 2017; pp.155-156. (In Turkish).
  • Kneifel W, Berger E. Microbiological criteria of random samples of spices and herbs retailed on the Austrian market. J Food Protection. 1994; 57(10): 893-901.
  • Kocak P. Investigation of microbiological quality of White, Tulum, Kashar and Lor cheeses produced in dairy factories and sold in Aydin province. Msc. Thesis, Adnan Menderes University, Health Sciences Institute, Aydın. 2014.
  • Larsen HD, Jørgensen K. The occurrence of Bacillus cereus in Danish pasteurized milk. International Journal of Food Microbiology. 1997; 34: 179- 186.
  • Lu Y, Shirashoji N, Lucey JA. Rheological, textural and melting properties of commercial samples of some of the different types of pasteurized processed cheese. International Journal of Dairy Technology, 2007; 60: 74-80.
  • McGuiggan JM, Gilmour A, Lawrence ML. Factors influencing the recovery of psychotropic, mesophilic and thermophilic Bacillus spp. from bulk raw milk. Journal of the Society of Dairy Technology. 1994; 47: 111- 116.
  • Oksuztepe G, Patir B, Dikici A, Ilhak OI. Microbiological and chemical quality of vacuum packaged fresh Kashar cheese in Elazığ. Fırat University Medical Journal of Health Sciences. 2009; 23(2): 89 – 94. Ozdemir C, Demirci M. Selected microbiological properties of Kashar cheese samples preserved with potassium sorbate. International Journal of Food Properties. 2006; 9: 515–521.
  • Piatkiewicz A, Fabijanska E. Microbial contamination of Fromaje Fin type cheese. 3rd world congress. Foodborne infection and intoxications. Vol. 2. 16-19 june, Berlin.1992. pp. 1028- 1030.
  • Ray B, Rhunia A. Basic Food Microbiology. 5th edition CRC. press. Boca Raton, London, New York. 2013.
  • Sekin Y, Karagozlu N. Food Microbiology. Basic Principles and Applications for Food. Industry Literature Publication, İstanbul, Turkey. 2004.
  • Shirashoji N, Abe T, Takahashi K, Iwatsuki K. 2006. Influence of emulsifying salts on functionality of sliced process cheese. Journal of Dairy Science. 2006; 89 (1): 423.
  • Tekinsen OC. Dairy Technology. Selçuk University Veterinary Faculty Publication. Konya, Turkey. 2000. (In Turkish).
  • Topal Ş. Risks of surface molds and mycotoxins in Kashar at the cheese ripening stage. Food. 1987; 12(3): 199-207.
  • Turantas F, Unluturk A, Goktan D. Microbiological and compositional status of Turkish White cheese. International Journal of Food Microbiology. 1989; 8(1): 19-24.Uçuncu M. A to Z Cheese Technology. Volume I. Ege University Meta Printing House, Izmir, Turkey. 2004. (in Turkish).
  • Wijman JGE, de Leeuw PPLA, Moezelaar R, Zwietering MH, Abee T. Air-liquid interface biofilms of Bacillus cereus: formation, sporulation and dispersion. Applied and Environmental Microbiology. 2007; 73: 1481-1488.
  • Yilmaz F, Dagdemir E. The effects of beeswax coating on quality of Kashar cheese during ripening. international Journal of Food Science and Technology. 2012; 47: 2582–2589.

Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province

Year 2019, , 235 - 241, 30.09.2019
https://doi.org/10.30607/kvj.557787

Abstract

In this study, the physicochemical and microbiological
quality of toast cheese offered for sale in markets in the center of
Afyonkarahisar province was examined. The acidity (pH) and water activity (aw)
values of the samples were determined to be 4.89 and 0.932 on average,
respectively. As a result of microbiological analyses, the total aerobic
bacteria, total aerobic psychrophilic bacteria, yeast and mold, total coliform
group bacteria, lactic acid bacteria counts, Lactococcus/Streptococcus spp., Pseudomonas
spp., Staphylococcus aureus, Bacillus
cereus
average counts were determined to be 5.81, 3.30, 4.14, 2.89, 4.69,
4.93, 4.74, 2.42 and <2.00 log CFU/g, respectively. Furthermore, the samples
were contaminated in percentages as follows: Escherichia coli 24%, Clostridium
perfringens
8%, Salmonella spp.
18%, and Listeria spp. 12%, while Brucella spp. growth did not occur in
the samples.

References

  • Akin N. Basic Cheese Science-1: Basic Topics. Damla Ofset Publications, Konya, Turkey. 2010. (In Turkish).
  • Anonymous. Turkish Standards Institute. TS 6235 EN ISO 6887-1. Microbiology of Food and Animal Feed General Rules of The Initial Suspension and Decimal Dilutions of Test Samples Preparation. 2001.
  • Anonymous. Turkish Food Codex. Cheese Communiqué, Communiqué No: 2015/6. 2015.
  • Aran N. A microbial study of Kashar cheese. Milchwissenschaft. 1998; 53: 565–567.
  • Aytac SA, Taban BM. Food-Borne Intoxications, In: Food Microbiology, Ed; Erkmen O. 5th Ed., Efil Publishing House, Ankara, Turkey. 2017; pp.155-156. (In Turkish).
  • Cetin B, Karasu S, Atik A, Durak MZ. Investigation of microbiological quality of some dairy products in Kırklareli: Detection of Salmonella spp. and Listeria monoctyogenes by Real Time PCR. Journal of Tekirdag Agricultural Faculty. 2015; 12(1): 74-80.
  • Cetinkaya A. Our Traditional Chesses. Abp Press, İstanbul, Turkey. 2008. (In Turkish).
  • Cetinkaya F, Soyutemiz GE. Microbiological and chemical changes throughout the manufacture and ripening of Kashar: a traditional Turkish cheese. Turk. J. Vet. Anim. Sci. 2006; 30: 397-404.
  • Corroler D, Mangin I, Desmasure N, Gueguen M. An ecological study of lactococci isolated from raw milk in the Camembert cheese registered designation of origin area. Appl. Environ. Microbiol. 1998; 64(12): 4729–4735.
  • Dogan HB, Tukel Cİ. Total (aerobic mesophilic) bacteria. Food Microbiology and applications, 2nd Ed., Sim Printing Co, Ankara, Turkey. 2000; pp. 323-328.(In Turkish).
  • Flowers RS, D’aust JY, Andrews WH, Bailey JS. Salmonella In: Compendium of the Methods for the Microbiological Examinations of Foods. Eds; Vanderzant C, Spilttstoesser DF. American Public Health Association. 1992; pp. 371-422.
  • Greenwood MH, Coetzee EF, Ford BM, Gill P, Hooper WL, Matthews SCV, Patrick S. The microbiology of selected retail food products with an evolation of vialable counting methods. Journal of Hygiene. 1984; 92: 67-77.
  • Guinee TP, Caric M, Kalab M. Pasteurized Processed Cheese and Substitute/Imitation Cheese Products, In: Cheese: Chemistry, Physics and Microbiology, Major Cheese Groups. Fox PF. Elsevier Applied Science, London, U.K. 2004; pp. 349-394.
  • Gulmez M, Oral N, Guven A, Baz E, Sezer C, Duman B. Some microbiological and chemical properties of kashar cheese sold in Kars. Journal of the Faculty of Veterinary Medicine, Kafkas University. 2004; 10 (2): 183-188.
  • Halkman K, Sagdas OE. Food Microbiology Applications. Prosigma Printing and Promotional Services, Ankara, Turkey. 2011.(in Turkish).
  • ISO. International Standard Organization. 4832, General guidance fort the enumeration of coliforms colony count technique. Geneva, Switzerland. 1991.
  • ISO. International Standard Organization. 6888-1, Horizontal method for the enumeration of coagulase- positive staphylococci technique using Baird Parker agar medium. Geneva, Switzerland. 1999.
  • ISO. International Standard Organization. 16649-1:2001, Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli , Part 1: Colony count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide. Geneva, Switzerland. 2001.
  • ISO. International Standard Organization. 7932:2004, Microbiology of food and animal feeding stuffs, Horizontal method for the enumeration of presumptive Bacillus cereus, Colony count technique at 30 degrees C. Geneva, Switzerland. 2004a.
  • ISO. International Standard Organization. 7937:2004, Microbiology of food and animal feeding stuffs, Horizontal method for the enumeration of Clostridium perfringens, Colony count technique. Geneva, Switzerland. 2004b.
  • ISO. International Standard Organization. 21527-1:2008, Microbiology of food and animal feeding stuffs, Horizontal method for the enumeration of yeasts and molds, Part 1: Colony count technique in products with water activity greater than 0,95. Geneva, Switzerland. 2008.
  • ISO. International Standard Organization. 13720:2010, specifies a method for the enumeration of presumptive Pseudomonas spp. present in meat and meat products, including poultry. Geneva, Switzerland. 2010.
  • ISO. International Standard Organization. 4833-1:2013, Microbiology of the food chain, Horizontal method for the enumeration of microorganisms, Part 1: Colony count at 30 degrees C by the pour plate technique. Geneva, Switzerland. 2013a.
  • ISO. International Standard Organization. 4833-2:2013, Horizontal method for the enumeration of microorganisms, Part 2: Colony count at 30 degrees C by the surface plating technique. Geneva, Switzerland. 2013b.
  • ISO. International Standard Organization. 16649-3:2015, Microbiology of the food chain, Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli, Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronide. Geneva, Switzerland. 2015.
  • ISO. International Standard Organization. 6579-1:2017, Horizontal method for the detection, enumeration and serotyping of Salmonella. Geneva, Switzerland. 2017a.
  • ISO. International Standard Organization. 11290-1:2017, Microbiology of the food chain, Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. Part 1: Detection method. Geneva, Switzerland. 2017b.
  • ISO. International Standard Organization. 11290-2:2017, Microbiology of the food chain, Horizontal method for the detection and enumeration of Listeria monocytogenes and of Listeria spp. , Part 2: Enumeration method. Geneva, Switzerland. 2017c.
  • Kara R. A traditional cheese: characterization of Afyon Tulum cheese and investigation of reproduction and viability of empirically inoculated Brucella abortus and Brucella melitensis strains. PhD thesis, Afyon Kocatepe University Health Sciences Institute, Afyonkarahisar, 2011.
  • Karagozlu N. Food Borne Toxi infections. In: Food Microbiology, Ed; Erkmen O. 5th Ed., Efil Publishing House, Ankara, Turkey. 2017; pp.155-156. (In Turkish).
  • Kneifel W, Berger E. Microbiological criteria of random samples of spices and herbs retailed on the Austrian market. J Food Protection. 1994; 57(10): 893-901.
  • Kocak P. Investigation of microbiological quality of White, Tulum, Kashar and Lor cheeses produced in dairy factories and sold in Aydin province. Msc. Thesis, Adnan Menderes University, Health Sciences Institute, Aydın. 2014.
  • Larsen HD, Jørgensen K. The occurrence of Bacillus cereus in Danish pasteurized milk. International Journal of Food Microbiology. 1997; 34: 179- 186.
  • Lu Y, Shirashoji N, Lucey JA. Rheological, textural and melting properties of commercial samples of some of the different types of pasteurized processed cheese. International Journal of Dairy Technology, 2007; 60: 74-80.
  • McGuiggan JM, Gilmour A, Lawrence ML. Factors influencing the recovery of psychotropic, mesophilic and thermophilic Bacillus spp. from bulk raw milk. Journal of the Society of Dairy Technology. 1994; 47: 111- 116.
  • Oksuztepe G, Patir B, Dikici A, Ilhak OI. Microbiological and chemical quality of vacuum packaged fresh Kashar cheese in Elazığ. Fırat University Medical Journal of Health Sciences. 2009; 23(2): 89 – 94. Ozdemir C, Demirci M. Selected microbiological properties of Kashar cheese samples preserved with potassium sorbate. International Journal of Food Properties. 2006; 9: 515–521.
  • Piatkiewicz A, Fabijanska E. Microbial contamination of Fromaje Fin type cheese. 3rd world congress. Foodborne infection and intoxications. Vol. 2. 16-19 june, Berlin.1992. pp. 1028- 1030.
  • Ray B, Rhunia A. Basic Food Microbiology. 5th edition CRC. press. Boca Raton, London, New York. 2013.
  • Sekin Y, Karagozlu N. Food Microbiology. Basic Principles and Applications for Food. Industry Literature Publication, İstanbul, Turkey. 2004.
  • Shirashoji N, Abe T, Takahashi K, Iwatsuki K. 2006. Influence of emulsifying salts on functionality of sliced process cheese. Journal of Dairy Science. 2006; 89 (1): 423.
  • Tekinsen OC. Dairy Technology. Selçuk University Veterinary Faculty Publication. Konya, Turkey. 2000. (In Turkish).
  • Topal Ş. Risks of surface molds and mycotoxins in Kashar at the cheese ripening stage. Food. 1987; 12(3): 199-207.
  • Turantas F, Unluturk A, Goktan D. Microbiological and compositional status of Turkish White cheese. International Journal of Food Microbiology. 1989; 8(1): 19-24.Uçuncu M. A to Z Cheese Technology. Volume I. Ege University Meta Printing House, Izmir, Turkey. 2004. (in Turkish).
  • Wijman JGE, de Leeuw PPLA, Moezelaar R, Zwietering MH, Abee T. Air-liquid interface biofilms of Bacillus cereus: formation, sporulation and dispersion. Applied and Environmental Microbiology. 2007; 73: 1481-1488.
  • Yilmaz F, Dagdemir E. The effects of beeswax coating on quality of Kashar cheese during ripening. international Journal of Food Science and Technology. 2012; 47: 2582–2589.
There are 45 citations in total.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section RESEARCH ARTICLE
Authors

Gökhan Akarca 0000-0002-5055-2722

Oktay Tomar This is me 0000-0001-5761-7157

Publication Date September 30, 2019
Acceptance Date June 9, 2019
Published in Issue Year 2019

Cite

APA Akarca, G., & Tomar, O. (2019). Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province. Kocatepe Veterinary Journal, 12(3), 235-241. https://doi.org/10.30607/kvj.557787
AMA Akarca G, Tomar O. Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province. kvj. September 2019;12(3):235-241. doi:10.30607/kvj.557787
Chicago Akarca, Gökhan, and Oktay Tomar. “Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province”. Kocatepe Veterinary Journal 12, no. 3 (September 2019): 235-41. https://doi.org/10.30607/kvj.557787.
EndNote Akarca G, Tomar O (September 1, 2019) Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province. Kocatepe Veterinary Journal 12 3 235–241.
IEEE G. Akarca and O. Tomar, “Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province”, kvj, vol. 12, no. 3, pp. 235–241, 2019, doi: 10.30607/kvj.557787.
ISNAD Akarca, Gökhan - Tomar, Oktay. “Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province”. Kocatepe Veterinary Journal 12/3 (September 2019), 235-241. https://doi.org/10.30607/kvj.557787.
JAMA Akarca G, Tomar O. Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province. kvj. 2019;12:235–241.
MLA Akarca, Gökhan and Oktay Tomar. “Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province”. Kocatepe Veterinary Journal, vol. 12, no. 3, 2019, pp. 235-41, doi:10.30607/kvj.557787.
Vancouver Akarca G, Tomar O. Investigation of Some Physicochemical and Microbiological Quality Parameters of Toast Cheese Sold Retail in Afyonkarahisar Province. kvj. 2019;12(3):235-41.

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