Investigation of Cytotoxic Effects of Curcuma Longa, Zingiberaceae and Dianthus Caryophllus, Which are Commonly Used as Food Supplements in Daily Life

Abstract

The aim of this study is to investigate the cytotoxic effects, which have an important place ,in terms of public health as using herbal food supplement, of ginger, turmeric and clove seeds over healthy cells. In this study, designed as an in vitro cell culture study, fibroblast cells were used to investigate the effect of plant extracts on healthy cells. MTT cell viability test was used to determine cytotoxicity. The MTT test was performed according to ISO 10993-5 standards and when the cell viability was calculated to be below 70% in the MTT method, the sample was considered potential cytotoxic. When the viability values calculated according to the absorbance values obtained from MTT test are examined; It was calculated that ginger and turmeric extracts were not cytotoxic at any concentration, including the highest concentrations. On the other hand, it was calculated that 1: 2 concentration of clove extract was cytotoxic and the viability was 26.7%. As a result of this study, it has been found that clove use is remarkable in terms of plant nutritional supplements with its current use in terms of cytotoxic effect compared to turmeric and ginger. 

Keywords

• Curcuma Longa
• Zingiberaceae
• Dianthus Caryophllus
• Cytoxicity

Günlük Yaşamda Gıda Takviyesi Olarak Kullanılan Zencefil, Zerdeçal ve Karanfilin Sitotoksik Etkilerinin Araştırılması

Abstract

Bu çalışmanın amacı, günlük yaşamda gıda takviyesi olarak kullanılan ve halk sağlığı açısından önemli bir yeri olan zencefil, zerdeçal ve karanfil tohumlarının sağlıklı hücreler üzerinde sitotoksik etkilerini araştırmaktır. İn vitro hücre kültürü çalışması olarak tasarlanan bu çalışmada, bitki özlerinin sağlıklı hücreler üzerindeki etkisini araştırmak için fibroblast hücreleri kullanılmıştır. Sitotoksisiteyi belirlemek için MTT hücre canlılığı testi kullanılmıştır. MTT testi ISO 10993-5 standartlarına göre yapıldı ve MTT yönteminde hücre canlılığı % 70'in altında olduğu hesaplandığında, numunenin sitotoksik potansiyelde olduğu kabul edilmiştir. MTT testinden elde edilen absorbans değerlerine göre hesaplanan canlılık değerleri incelendiğinde; Zencefil ve zerdeçal ekstrelerinin, en yüksek konsantrasyonlar dahil olmak üzere herhangi bir konsantrasyonda sitotoksik olmadığı hesaplanmıştır. Öte yandan, 1:2 oranında karanfil ekstresi konsantrasyonunun sitotoksik ve canlılığın % 26.7 olduğu hesaplanmıştır. Bu çalışma sonucunda, özellikle karanfil kullanımının zerdeçal ve zencefil ile karşılaştırıldığında sitotoksik etkiye sahip olması bakımından mevcut kullanımıyla, bitkisel gıda takviyeleri açısından dikkate değer olduğu tespit edilmiştir.

Keywords

• Zerdeçal
• zencefil
• karanfil
• sitotoksitite

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