Afyonkarahisar’da Satışa Sunulan Tavuk İç Organlarından Salmonella spp. ve Listeria monocytogenes’in İmmonomagnetik Seperasyon Yöntemi ile İzolasyonu ve İzolatların Antibiyotik Dirençliliğinin Belirlenmesi

Year 2021, , 201 - 209, 30.06.2021

Özgür Sepin Şebnem Pamuk

https://doi.org/10.30607/kvj.839629

Abstract

Bu çalışmada, Ağustos 2013-Şubat 2015 tarihleri arasında Afyonkarahisar’da tüketime sunulan 100’er adet yürek, taşlık ve ciğerden oluşan toplam 300 tavuk iç organ örneğinde, L. monocytogenes ve Salmonella türlerinin immunomagnetik seperasyon tekniği ile saptanması, izolatların antibiyotik duyarlılık profillerinin belirlenmesi amaçlanmıştır. İç organ örneklerinden %13 Salmonella spp. ve %2,6 L. monocytogenes saptandı. Elde edilen 39 Salmonella spp. izolatının 9’u yürekten (%23,2), 1’i taşlıktan (%2,5), 29’u (%74,3) karaciğerden izole edildi. L. monocytogenes’in iç organ çeşidine göre izolat sayıları sırasıyla; yürekten 2 (%25), taşıktan 5 (%62,5) ve karaciğerden 1 (%12,5) adet olarak belirlendi. Salmonella spp. izolatlarının %100’ünün tetrasiklin, klortetrasiklin ve oksitetrasiklin’e, %64,1’inin penisilin’e, %48,7’si ampisilin’e, %46,1’inin florfenikol’e, %43,5’inin amoksisilin’e, %26’sının streptomisin’e, %15,3’ünün enrofloksasin’e, %7,6’sının trimetoprim/sülfadiazin’e dirençli olduğu saptanırken, gentamisin dirençliliği gözlendi. Elde edilen L. monocytogenes izolatlarının %62,5’inin tetrasiklin, klortetrasiklin, oksitetrasiklin ve trimetoprim/sülfadiazin’e, %50’sinin amoksisilin’e, florfenikol’e, %37,5’inin enrofloksasin’e, %25’inin ampisilin ve penisilin’e, %12,5’inin streptomisin’e dirençli olduğu belirlendi. Salmonella spp. ve L. monocytogenes izolatlarının hiçbirinde gentamisin direnci saptanmadı. Salmonella spp. ve L. monocytogenes izolatlarının en az bir ve daha fazla antibiyotiğe çoklu dirençli oldukları belirlendi.

Keywords

İç organ, Antibiyotik direnci, IMS, Salmonella spp., L.monocytogenes

Supporting Institution

Afyon Kocatepe Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

13 SAĞBİL 06

Isolation of Salmonella spp. and Listeria monocytogenes from Chicken Internal Organs Retailed in Afyonkarahisar by Immonomagnetic Seperation Method and Determination of Antibiotic Resistance of Isolates

Abstract

This study consists of 100 hearts and gizzards offered for retailed in Afyonkarahisar between August 2013 and February 2015. It was aimed to determine L. monocytogenes and Salmonella species by immunomagnetic separation technique in a total of 300 chicken internal organs and to determine the antibiotic susceptibility profiles of the isolates. 13% (39/300) Salmonella spp. 2.6% L. monocytogenes were detected in internal organ samples. The 39 Salmonella spp. 9 of the isolates were isolated from the heart (23.2%), 1 from the gizzard (2.5%), 29 (74.3%) from the liver. According to the internal organ type of L. monocytogenes isolate numbers are; 2 (25%) from the heart, 5 (62.5%) from the stone, and 1 (12.5%) from the liver. Salmonella spp. 100% of its isolates are tetracycline, chlortetracycline, and oxytetracycline, 64.1% to penicillin, 48.7% to ampicillin, 46.1% to florphenicol, 43.5% to amoxicillin resistance was found to be 26% to streptomycin, 15.3% to enrofloxacin and 7.6% to trimethoprim/sulfadiazine, whereas resistance to gentamicin was observed. 62.5% of isolates to tetracycline, chlortetracycline, oxytetracycline and trimethoprim / sulfadiazine, 50% to amoxicillin, florphenicol, 37.5% to enrofloxacin, 25% to ampicillin and penicillin 12.5% were found to be resistant to streptomycin. Gentamicin resistance was not detected in any of the Salmonella spp. and L. monocytogenes isolates.

Keywords

Antibiotic resistance, Internal organ, Salmonella spp., L. monocytogenes

Project Number

13 SAĞBİL 06

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